##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294852_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104191
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.26734554807997	33.0	30.0	34.0	15.0	34.0
2	29.595924791968596	33.0	30.0	34.0	16.0	34.0
3	29.977051760708697	33.0	30.0	34.0	18.0	34.0
4	32.50800932902074	37.0	35.0	37.0	17.0	37.0
5	33.60292155752416	37.0	35.0	37.0	27.0	37.0
6	33.217840312503	37.0	35.0	37.0	20.0	37.0
7	33.55810962559146	37.0	35.0	37.0	24.0	37.0
8	33.38224990642186	37.0	35.0	37.0	22.0	37.0
9	34.467314835254484	39.0	35.0	39.0	17.0	39.0
10	34.849535948402455	39.0	35.0	39.0	21.0	39.0
11	34.73534182414988	39.0	35.0	39.0	19.0	39.0
12	35.0405505273968	39.0	35.0	39.0	24.0	39.0
13	34.60525381270935	39.0	35.0	39.0	18.0	39.0
14	35.907333646860096	41.0	37.0	41.0	19.0	41.0
15	35.919254062251056	41.0	37.0	41.0	19.0	41.0
16	35.903868856235185	40.0	36.0	41.0	20.0	41.0
17	35.942960524421494	40.0	36.0	41.0	21.0	41.0
18	35.87854997072684	40.0	36.0	41.0	22.0	41.0
19	35.84840341296273	40.0	35.0	41.0	22.0	41.0
20	35.7961628163661	40.0	35.0	41.0	22.0	41.0
21	35.76808937432216	40.0	35.0	41.0	22.0	41.0
22	35.854210056530796	40.0	35.0	41.0	23.0	41.0
23	35.858557840888366	40.0	35.0	41.0	24.0	41.0
24	36.0486510351182	40.0	35.0	41.0	25.0	41.0
25	35.34993425535795	40.0	33.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.26619356722183696
1101	2	0.09994063341822823
1101	3	-0.023759157141910237
1101	4	-0.30818516863840273
1101	5	-0.04238718247795248
1101	6	-0.48994857549159576
1101	7	0.30990561414066065
1101	8	0.14901872012219286
1101	9	0.42486176765267913
1101	10	0.8892153277303265
1101	11	0.3490054860977665
1101	12	-0.12683426307493306
1101	13	0.6347377814881128
1101	14	0.45528825301528997
1101	15	0.40966502582126907
1101	16	0.13280290989193588
1101	17	0.0647719377742817
1101	18	0.18530072683634558
1101	19	0.5992697974298764
1101	20	0.27881814544512906
1101	21	-0.16915090496679142
1101	22	-0.18443319357236732
1101	23	-0.3188805054099646
1101	24	0.01374891542805301
1101	25	-0.4443800132329514
1102	1	-1.2101846291085572
1102	2	-0.806209177132633
1102	3	-0.3990141848306301
1102	4	-0.7684980026032306
1102	5	-0.4798689662144682
1102	6	-0.3853143580622742
1102	7	-0.19354333174489113
1102	8	-0.19626448205404046
1102	9	-0.3652868770573221
1102	10	-0.26618154393002413
1102	11	-1.022800595705398
1102	12	-1.382250565416335
1102	13	-1.0660297304131845
1102	14	-1.247179443477478
1102	15	-0.7245524034996293
1102	16	-1.2094199600196518
1102	17	-1.3931606377624917
1102	18	-1.24863495760939
1102	19	-0.8915977824788044
1102	20	-0.9474521353282128
1102	21	-1.4955863465290165
1102	22	-1.5191752512814887
1102	23	-1.6213676325837696
1102	24	-1.4649744888272664
1102	25	-1.4725545007487426
1103	1	-1.2761871197685828
1103	2	-0.9297637047967484
1103	3	-1.072115056561639
1103	4	-0.977891348982876
1103	5	-0.7337383844674505
1103	6	-1.195668387594381
1103	7	-0.8830113979252872
1103	8	-1.8787053288784534
1103	9	-1.3241020345026158
1103	10	-1.3483733247519396
1103	11	-0.8440957389183481
1103	12	-0.5499253563787931
1103	13	-0.49130992966598797
1103	14	-1.1684567962616796
1103	15	-2.071821572686609
1103	16	-1.3081568395784515
1103	17	-2.3029631640033728
1103	18	-2.0999069732649716
1103	19	-2.441855743695669
1103	20	-2.0464558713182512
1103	21	-1.8374294349474454
1103	22	-1.5965636163410863
1103	23	-1.7450111123916372
1103	24	-1.2441952680480455
1103	25	-0.9943477759666735
1104	1	-0.7341808501761058
1104	2	-0.82170280018412
1104	3	-0.8156887242732402
1104	4	-0.8044923342045536
1104	5	-1.016855267584333
1104	6	-1.3242845989692142
1104	7	-1.8666656746830128
1104	8	-1.4918714730790796
1104	9	-1.323340726844755
1104	10	-1.2828560833726286
1104	11	-1.1973872749685057
1104	12	-1.2125764983402831
1104	13	-0.8345701177537563
1104	14	-1.0912512431940513
1104	15	-1.716380462073083
1104	16	-0.8945876501471943
1104	17	-1.3068592678994762
1104	18	-1.6511385003585701
1104	19	-1.3271669841793212
1104	20	-1.2868947427916027
1104	21	-0.8458933847907062
1104	22	-1.4830392097132332
1104	23	-1.5997804809541094
1104	24	-1.65462966124106
1104	25	-2.0877647038663625
1105	1	0.39840649853364596
1105	2	0.30129331765100176
1105	3	0.07839668335407168
1105	4	0.18577941733689585
1105	5	0.038773871488515965
1105	6	0.32166196417305315
1105	7	0.051580500242771166
1105	8	-0.26040310130470345
1105	9	-0.4739687241044024
1105	10	0.052288926903692357
1105	11	-0.11832812904478374
1105	12	0.22030772180130498
1105	13	0.17365093728163572
1105	14	-0.4263049084144228
1105	15	-0.12802695670656306
1105	16	-0.3369260835794847
1105	17	-0.277720195051792
1105	18	0.04815357545783172
1105	19	-0.3102224763008081
1105	20	-0.7017839869112663
1105	21	0.19297393651552852
1105	22	-0.34168273563238927
1105	23	-0.2945509765010357
1105	24	-0.24312018419150405
1105	25	-0.6246909427336789
1106	1	0.08336998978852961
1106	2	-0.45706973210277724
1106	3	-0.5837700682166513
1106	4	-0.24803786912939785
1106	5	-0.174547575276641
1106	6	-0.7282191701451666
1106	7	-0.3151625800764677
1106	8	-0.2947892449623666
1106	9	-0.5372222476228288
1106	10	-0.1752964016750198
1106	11	-0.46414235896497047
1106	12	-0.1131954196488607
1106	13	-0.7622889506450079
1106	14	-0.5490761768810586
1106	15	-0.35179160265663967
1106	16	-0.8536946351162484
1106	17	-0.41967978071998857
1106	18	-0.507416130774125
1106	19	-0.5353455871855104
1106	20	-0.06160738646976682
1106	21	-0.18824528576330124
1106	22	-0.36787563765310693
1106	23	-0.25150461888514286
1106	24	-0.6829232067759818
1106	25	0.10471982310092187
1107	1	-0.3294167848403511
1107	2	-0.23726541361424935
1107	3	0.30168877118955706
1107	4	0.27304850064118114
1107	5	0.1397236797840833
1107	6	0.08116004712879032
1107	7	0.06709113112460585
1107	8	0.09438079552346323
1107	9	0.28278450001550226
1107	10	0.4476793068270055
1107	11	0.542267897445285
1107	12	0.30448339276679803
1107	13	0.15522117081248155
1107	14	0.30770519963716225
1107	15	-0.18579969982263123
1107	16	0.041210897947181024
1107	17	0.08656178473210474
1107	18	0.09464189891548358
1107	19	0.48962067462285574
1107	20	0.22567331528462375
1107	21	0.19367104352144793
1107	22	0.05030924763703837
1107	23	0.3340216969043297
1107	24	0.16002551117273356
1107	25	0.4105565302534231
1108	1	-0.37270788295601065
1108	2	-0.20778198281502824
1108	3	-0.08614423725748566
1108	4	-0.3888260932509553
1108	5	0.40166325093418465
1108	6	-0.5309257228517197
1108	7	-0.6278406427545349
1108	8	0.11736650052671393
1108	9	-0.22525643565701614
1108	10	0.47878716907521834
1108	11	-0.23242346057940466
1108	12	0.09525662213651742
1108	13	-0.3313772697333235
1108	14	-0.7950400895116374
1108	15	-0.6563974905958574
1108	16	-0.8045493359709468
1108	17	-0.30192099629454106
1108	18	-0.45969533305333243
1108	19	-0.7093721778787696
1108	20	-1.4134592383216145
1108	21	-0.5652633294480083
1108	22	-0.3576667107775151
1108	23	-0.6774305448110667
1108	24	-0.7736164641359053
1108	25	-0.6312725795581429
1109	1	1.0163262492153748
1109	2	0.77566028298018
1109	3	0.6776175637592168
1109	4	0.9153859619414888
1109	5	0.9007917912391932
1109	6	0.8594882205034011
1109	7	0.9091963942825032
1109	8	0.6726315695172715
1109	9	0.8819331068266436
1109	10	0.728097263483356
1109	11	0.4334443680335198
1109	12	0.8326567673798024
1109	13	0.8135029045586109
1109	14	1.0549962213089898
1109	15	0.7846337367098783
1109	16	0.6398951084734961
1109	17	0.6519642615122905
1109	18	0.767319917491335
1109	19	0.8306423464494799
1109	20	0.9364318292165095
1109	21	0.9989342014161906
1109	22	0.9352163683800754
1109	23	0.8837894987619137
1109	24	1.1703196288197901
1109	25	0.7682190689531154
1110	1	0.01150345053068591
1110	2	0.005583556637468234
1110	3	-0.07437366751590346
1110	4	-0.7356553241381576
1110	5	-0.20691737938557964
1110	6	-0.07934992344983272
1110	7	0.4650970154005165
1110	8	-0.10964829895185346
1110	9	-0.23008735353140963
1110	10	0.2075272963660737
1110	11	-0.4095954565919797
1110	12	-0.3266051982760274
1110	13	0.2605251917343523
1110	14	0.21155449679309157
1110	15	0.4197086362349012
1110	16	0.05293858673436347
1110	17	-0.21741827411065628
1110	18	0.19512408263565106
1110	19	0.07960106036983206
1110	20	-0.4239262270494777
1110	21	-0.3159726308819515
1110	22	0.15414219958211817
1110	23	0.09321587800926778
1110	24	-0.1450987124297498
1110	25	0.5465895475793445
1111	1	0.9889206048767143
1111	2	0.6137765699573983
1111	3	0.4407593522665252
1111	4	0.8032812356579129
1111	5	0.4178268133405183
1111	6	-0.055438675110337954
1111	7	0.21266460237498563
1111	8	0.43553534374372305
1111	9	-0.013952646318543316
1111	10	0.4233029758260969
1111	11	0.33851067201175766
1111	12	0.26934496867188074
1111	13	0.23937845709176742
1111	14	0.5651968333457091
1111	15	0.183001638783999
1111	16	0.0474095593405508
1111	17	-0.11495175344860797
1111	18	0.39183538841958665
1111	19	0.9480173889520316
1111	20	1.0000496462855324
1111	21	0.6919977852312158
1111	22	1.0734686635177795
1111	23	0.8207497163749693
1111	24	1.0176844707103072
1111	25	1.1317688819418947
1112	1	-0.4115235347660118
1112	2	-0.7460745126329797
1112	3	0.0018448818050771365
1112	4	-0.9222012763436531
1112	5	-0.662053007697331
1112	6	-0.37022264689440476
1112	7	-0.5512555831186248
1112	8	-0.5083920263617614
1112	9	-1.2497385448848846
1112	10	-1.4133450761643687
1112	11	-0.865924192950196
1112	12	-1.2731258846640685
1112	13	-1.7971650914533512
1112	14	-1.3934916484151714
1112	15	-0.9603313679760674
1112	16	-1.2329862474587046
1112	17	-1.2345428837186887
1112	18	-1.1172596770583496
1112	19	-1.10694489183566
1112	20	-0.9203644486166596
1112	21	-1.2208398098832447
1112	22	-1.182630092238071
1112	23	-0.7402051838568937
1112	24	-0.4677993475843252
1112	25	-1.5100129018012893
1113	1	-0.11051399507906368
1113	2	-0.013185747235183953
1113	3	-0.003063889149817811
1113	4	0.22117188286723888
1113	5	0.5962594865140289
1113	6	0.060452540657692566
1113	7	0.08679202269780717
1113	8	-0.2731414938064205
1113	9	0.5743792656180275
1113	10	0.5177468938272938
1113	11	0.02450833460375179
1113	12	0.1242993260426104
1113	13	0.0936379093802131
1113	14	0.6404992749894731
1113	15	-0.18270795406479579
1113	16	0.20546887896529853
1113	17	0.06035384685461764
1113	18	0.33295797599342336
1113	19	0.6265922018014578
1113	20	0.47937622008761593
1113	21	0.08035496644350815
1113	22	0.2301697595726182
1113	23	0.35778499860119695
1113	24	0.16617305215633849
1113	25	-0.03756685197263465
1114	1	0.3098079662265043
1114	2	1.1052310301979844
1114	3	0.8079832235366418
1114	4	0.7912206201290886
1114	5	0.610171759442693
1114	6	0.7707759788499828
1114	7	0.5281153132014254
1114	8	0.623505973332847
1114	9	1.147115936715359
1114	10	0.6489239725453544
1114	11	1.1301057352831236
1114	12	0.31230700585356885
1114	13	0.8588129204568631
1114	14	0.8675558397509562
1114	15	0.8797052788402411
1114	16	0.9074626760410638
1114	17	0.8390866780464705
1114	18	0.28735171399371495
1114	19	0.8172878154478909
1114	20	1.1149691901068124
1114	21	0.37460987709186355
1114	22	0.9492068294293574
1114	23	1.2863332189526915
1114	24	0.5755660517132739
1114	25	0.9437357621168658
1115	1	0.21491959959900697
1115	2	0.5273226870722496
1115	3	0.3557505560865799
1115	4	-0.4297547030201443
1115	5	-0.08968135454085768
1115	6	-0.06071638307716398
1115	7	-0.2049340405001061
1115	8	-0.25172904429346943
1115	9	-0.5996525709227143
1115	10	-0.4625037595345489
1115	11	-0.055558189511238254
1115	12	-0.1589033349219875
1115	13	-0.5733257399426677
1115	14	-0.35133370196466984
1115	15	0.1992117418249677
1115	16	-0.10467010891780859
1115	17	0.3431407321005224
1115	18	-0.07040386767490503
1115	19	0.28224081192262673
1115	20	-0.10327405313643112
1115	21	0.7663255057640157
1115	22	0.25196828653864145
1115	23	0.9169736419844199
1115	24	0.8828515981292568
1115	25	0.8959684295669206
1116	1	-0.5397213383589801
1116	2	-0.2955888908466555
1116	3	-0.0036778757649074123
1116	4	-0.17291542602942656
1116	5	0.3494368672471282
1116	6	-0.38069684228575795
1116	7	-0.4503541675152505
1116	8	-0.2208912328621082
1116	9	-0.43624270282305844
1116	10	-0.42702501014520067
1116	11	-1.0054849115434763
1116	12	-0.5524060217808113
1116	13	-0.5939657008610837
1116	14	0.272444998572702
1116	15	-0.5106075058545656
1116	16	-0.062352082537742604
1116	17	-0.2607918521691417
1116	18	-0.1271942926871219
1116	19	0.0524533180291229
1116	20	-0.3911954014510357
1116	21	-0.2797174839770733
1116	22	-0.4667664472180775
1116	23	-0.47060574248065024
1116	24	-0.4144126910744603
1116	25	-0.3625806666714766
1201	1	-0.14229720016437497
1201	2	0.3927471177140731
1201	3	-0.18528759830561015
1201	4	-0.18877570272437083
1201	5	0.10423316778981473
1201	6	0.4524454033765508
1201	7	-0.026177755377361223
1201	8	0.006885270997862847
1201	9	-0.11414532454590187
1201	10	0.1516266752480604
1201	11	-0.12950081003770464
1201	12	0.1578668514134165
1201	13	0.11382303260983662
1201	14	3.743725694889122E-4
1201	15	0.2537813957735011
1201	16	0.27594953024220104
1201	17	-0.0089000842260063
1201	18	-0.24616547666633437
1201	19	-0.22385945427080856
1201	20	-0.5922196313677262
1201	21	-0.29970525436612405
1201	22	-0.21468359160392225
1201	23	-0.3501440746674547
1201	24	-0.4275595228408733
1201	25	-0.9609525997143535
1202	1	0.7084566148751534
1202	2	0.7714472464142013
1202	3	0.43771295326637016
1202	4	-0.06959019068172267
1202	5	0.5723339216048515
1202	6	0.19780300587701305
1202	7	0.44973693482304355
1202	8	1.1532357030625775
1202	9	0.6944132340126572
1202	10	0.6651401887615762
1202	11	0.46118681636420433
1202	12	0.3731178166643758
1202	13	0.5209750826190245
1202	14	0.7107990829941997
1202	15	0.5634890626240292
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2210	12	0.11197940552596464
2210	13	-0.003431141787196168
2210	14	1.0523224245175413
2210	15	0.7935463926813568
2210	16	0.8671678357462227
2210	17	0.9234437624035507
2210	18	0.8052878319298316
2210	19	1.3689667671268424
2210	20	1.186877462015083
2210	21	1.0262069286889144
2210	22	0.8774623576848839
2210	23	0.9636468962663756
2210	24	0.8532073293545537
2210	25	0.7632279816090843
2211	1	0.33474361616215376
2211	2	0.3570328319997884
2211	3	-0.02882501327159659
2211	4	0.545918174826646
2211	5	0.6510018752728115
2211	6	0.9320372401112422
2211	7	0.4330059180920287
2211	8	0.6965111213379984
2211	9	0.7191880087874267
2211	10	0.4849600085813961
2211	11	0.542267897445285
2211	12	0.4495335180800808
2211	13	0.5449454815894299
2211	14	0.2444219916171093
2211	15	0.7869446610796231
2211	16	0.15179987037825526
2211	17	0.43242644638623773
2211	18	0.45366445530645905
2211	19	0.1171269402870152
2211	20	-0.13898833885071582
2211	21	0.40012467760666226
2211	22	0.6160987213212508
2211	23	0.08778109540057244
2211	24	0.10645408260130296
2211	25	-0.07064647726537743
2212	1	0.32099156361699954
2212	2	0.36565352165495923
2212	3	0.32913886193365727
2212	4	-0.0435727939746684
2212	5	0.003834387588078414
2212	6	0.4550257458583715
2212	7	-0.03907946778646476
2212	8	-0.05164158802161012
2212	9	-0.18311084178728265
2212	10	-0.566764129349643
2212	11	-0.34566313703747653
2212	12	-0.502231670754071
2212	13	-0.07020230166180852
2212	14	-0.3567684845733652
2212	15	0.24568850344415694
2212	16	-1.9290704087678847
2212	17	-0.8896178885891359
2212	18	-0.16723045438155282
2212	19	0.20846519972872812
2212	20	0.26597882042678833
2212	21	0.20123305199089714
2212	22	0.3790297558040123
2212	23	0.5415988293907219
2212	24	0.6154852812876754
2212	25	0.4110858708462857
2213	1	-1.2135897171711783
2213	2	-1.0739195489525954
2213	3	-1.0669202513668345
2213	4	-1.1778913489828788
2213	5	-0.8511409818700457
2213	6	-1.1203437122697082
2213	7	-0.8593750342889237
2213	8	-1.0989650691381954
2213	9	-1.0989072293078124
2213	10	-1.1626590390376563
2213	11	-0.9534463882690005
2213	12	-1.259276005729447
2213	13	-1.1991021374581905
2213	14	-1.0110541988590782
2213	15	-1.7094839103489434
2213	16	-0.8429620343836461
2213	17	-1.3297164107566175
2213	18	-0.4970498304078248
2213	19	-0.7854921073320327
2213	20	-0.842559767422145
2213	21	-0.7867800842981012
2213	22	-0.9417584215358943
2213	23	-0.7572189045994264
2213	24	-0.6849744888272653
2213	25	-1.1070750486939502
2214	1	0.2378388542573937
2214	2	0.194651879618835
2214	3	0.5159368914903091
2214	4	-0.30931992041144696
2214	5	0.8060018752728055
2214	6	0.6396562877302898
2214	7	0.02633925142536242
2214	8	0.24389207371895338
2214	9	0.712521342120759
2214	10	-0.019801896180510425
2214	11	0.19512504030242894
2214	12	0.5235811371276995
2214	13	0.1837550053989503
2214	14	-0.14248277028764988
2214	15	0.35908751822248064
2214	16	0.24846653704492638
2214	17	0.521712160671953
2214	18	0.8172358838778848
2214	19	0.08879360695368632
2214	20	0.32315451829214226
2214	21	0.464648487130475
2214	22	0.2868130070355335
2214	23	0.4256382382577115
2214	24	0.4121683683155908
2214	25	1.0414963798774792
2215	1	1.1955832151596475
2215	2	1.082471428491015
2215	3	1.3503729817158714
2215	4	1.666469553272762
2215	5	1.4778815745209286
2215	6	0.9390547839708958
2215	7	1.2776174469140855
2215	8	1.540433427102407
2215	9	1.599889763173394
2215	10	1.9147845699849029
2215	11	1.8080573711294932
2215	12	1.493957076977324
2215	13	1.4986422234440653
2215	14	2.05375783121611
2215	15	1.7799897738615797
2215	16	1.6714740558419194
2215	17	2.087877574205784
2215	18	1.7354313725996917
2215	19	1.6869890956754858
2215	20	1.761199631074092
2215	21	1.7252499908898713
2215	22	1.9621513529002002
2215	23	1.732705907430649
2215	24	1.677130774330628
2215	25	1.4487144249902641
2216	1	0.5083978604685733
2216	2	0.21754007837659728
2216	3	-0.17685813956559215
2216	4	-0.43518948562883963
2216	5	0.23097081937218888
2216	6	0.05341405170544533
2216	7	-0.23210795354358282
2216	8	0.28087965135870263
2216	9	0.34056482038162983
2216	10	0.44510493611762314
2216	11	0.22161572353223846
2216	12	-0.3328163784002527
2216	13	-0.3015866095078792
2216	14	-0.3948119628342326
2216	15	-0.3660056494793835
2216	16	0.13989510847349607
2216	17	-0.0481636157255636
2216	18	0.09807439319465772
2216	19	-0.22862875329476395
2216	20	0.33150855555921765
2216	21	0.5815428970683598
2216	22	0.5128378517560321
2216	23	0.6506692941583339
2216	24	0.34480811986838233
2216	25	0.5970553860886625
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96.0
3	13.0
4	8.0
5	16.0
6	9.0
7	30.0
8	16.0
9	27.0
10	67.0
11	150.0
12	236.0
13	390.0
14	769.0
15	1205.0
16	1684.0
17	1885.0
18	1817.0
19	1807.0
20	1611.0
21	1530.0
22	1369.0
23	1242.0
24	1023.0
25	1023.0
26	926.0
27	855.0
28	754.0
29	726.0
30	746.0
31	899.0
32	1041.0
33	1304.0
34	1681.0
35	2388.0
36	4429.0
37	12124.0
38	58295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.77174559904782	29.81609107139429	11.043174444721737	19.368988884836153
2	12.835037170252258	34.59285831065291	29.54097383175773	23.03113068733711
3	18.112917187650286	40.69731653361547	23.61258055208233	17.57718572665192
4	11.730425249552825	31.995653261015907	25.61979497240013	30.654126517031138
5	10.023565623046217	49.10883470398692	27.25147886307892	13.616120809887944
6	24.3272087455652	44.39412731835357	14.364417779572527	16.914246156508696
7	17.96486639808467	47.6342028595329	17.472572906550774	16.928357835831658
8	21.411956939638603	44.57420222991158	17.345251826220682	16.668589004229144
9	21.800665346230026	31.4818375862931	16.572120839182354	30.145376228294523
10	15.771202169501958	42.74572783136353	23.363497360246953	18.119572638887554
11	22.331907531119334	39.72317008698995	15.317921853222474	22.627000528668237
12	19.285748618120643	43.98269646719539	22.13121845710166	14.60033645758231
13	24.25414045819147	35.996499124781195	19.29905553311405	20.450304883913287
14	18.796211720590357	40.129801451853275	20.963415220422096	20.110571607134272
15	26.862543384834296	36.100027881666364	19.100864331657228	17.936564401842112
16	16.00169207991232	40.29457573019017	25.454265771915317	18.249466417982195
17	19.982886096395504	40.40611870126624	21.892336240133066	17.718658962205193
18	19.171642006287676	35.61093324872853	25.93521963600704	19.282205108976765
19	19.354280606311093	40.02921981180134	26.152692740222417	14.463806841665145
20	21.12722098300131	35.029036227982466	24.734635797246366	19.109106991769863
21	19.427697810597973	37.39771732964106	25.86032826607437	17.3142565936866
22	20.98828323993887	36.57282365266871	21.46983342784917	20.969059679543246
23	18.18968418016632	36.39475075710234	25.260779695236263	20.154785367495073
24	20.088264138607386	39.371767013441534	22.66119262350249	17.87877622444859
25	19.3679554169041	35.29849232641471	22.344669055506728	22.98888320117446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7854.0
1	7854.0
2	5332.5
3	2811.0
4	2811.0
5	2811.0
6	2460.0
7	2109.0
8	2109.0
9	2109.0
10	1999.0
11	1889.0
12	1889.0
13	1889.0
14	1769.0
15	1649.0
16	1649.0
17	1649.0
18	1967.5
19	2286.0
20	2286.0
21	2286.0
22	2734.5
23	3183.0
24	3183.0
25	3183.0
26	3928.0
27	4673.0
28	4673.0
29	4673.0
30	5249.0
31	5825.0
32	5825.0
33	5825.0
34	5996.0
35	6167.0
36	6167.0
37	6167.0
38	5837.5
39	5508.0
40	5508.0
41	5508.0
42	6185.5
43	6863.0
44	6863.0
45	6863.0
46	13803.5
47	20744.0
48	20744.0
49	20744.0
50	18122.5
51	15501.0
52	15501.0
53	15501.0
54	11605.5
55	7710.0
56	7710.0
57	7710.0
58	5766.0
59	3822.0
60	3822.0
61	3822.0
62	3348.0
63	2874.0
64	2874.0
65	2874.0
66	2166.5
67	1459.0
68	1459.0
69	1459.0
70	1083.5
71	708.0
72	708.0
73	708.0
74	539.5
75	371.0
76	371.0
77	371.0
78	255.5
79	140.0
80	140.0
81	140.0
82	85.5
83	31.0
84	31.0
85	31.0
86	20.5
87	10.0
88	10.0
89	10.0
90	6.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008637982167365704
2	0.20155291723853308
3	0.2121104509986467
4	0.19675403825666324
5	0.21690932998051654
6	0.17659874653280994
7	0.18043784971830581
8	0.14492614525246902
9	0.1775585223291839
10	0.19579426246028928
11	0.14972502423433884
12	0.15932278219807852
13	0.20827134781315082
14	0.17851829812555786
15	0.17275964334731408
16	0.16988031595819217
17	0.174679194940062
18	0.17084009175456613
19	0.145885921048843
20	0.17563897073643597
21	0.18235740131105374
22	0.145885921048843
23	0.16892054016181818
24	0.1775585223291839
25	0.6276933708285745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	104191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.3480147037652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35012726277981	32.01620101544279
2	11.829491662503866	9.545930070735476
3	3.9201693665405934	4.7451315372728935
4	1.6389543043364496	2.645142094806653
5	0.8087728062037631	1.6316188538357441
6	0.523323580484788	1.2669040512136365
7	0.2735555079806846	0.7726195160810434
8	0.22598063702752208	0.729429605244215
9	0.17126953543138515	0.6219347160503306
>10	1.0038297771117297	8.263669606779857
>50	0.11180094673993196	3.27091591404248
>100	0.09990722900164134	9.683178009616954
>500	0.028544922571897526	8.936472440038006
>1k	0.011893717738290635	8.60343023869624
>5k	0.0023787435476581268	7.267422330143678
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	7572	7.267422330143678	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2735	2.6249868030828	No Hit
CCCATGTACTCTGCGTTGATACCAC	2534	2.4320718680116324	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1509	1.4483016767283161	No Hit
CATGTACTCTGCGTTGATACCACTG	1118	1.0730293403460953	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1068	1.0250405505273967	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	973	0.93386184987187	No Hit
GCGTTGATACCACTGCTTCCCATGT	936	0.8983501454060331	No Hit
GGTATCAACGCAGAGTACATGGGAA	924	0.8868328358495455	No Hit
GGTATCAACGCAGAGTACTTTTTTT	863	0.8282865122707336	No Hit
ACTCTGCGTTGATACCACTGCTTCC	835	0.8014127899722625	No Hit
TATCAACGCAGAGTACATGGGAAGC	818	0.785096601433905	No Hit
GTATCAACGCAGAGTACATGGGAAG	767	0.7361480358188327	No Hit
GTGGTATCAACGCAGAGTACATGGG	758	0.727510053651467	No Hit
TATCAACGCAGAGTACTTTTTTTTT	678	0.6507279899415497	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	658	0.6315324740140703	No Hit
GTACTCTGCGTTGATACCACTGCTT	562	0.5393939975621695	No Hit
GCTTCCCATGTACTCTGCGTTGATA	539	0.5173191542455683	No Hit
GATACCACTGCTTCCCATGTACTCT	491	0.47124991601961785	No Hit
ATACCACTGCTTCCCATGTACTCTG	451	0.4328588841646591	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	416	0.3992667312915703	No Hit
CAGTGGTATCAACGCAGAGTACATG	405	0.38870919753145666	No Hit
CATGGGAAGCAGTGGTATCAACGCA	402	0.3858298701423347	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	389	0.3733527847894732	No Hit
ACATGGGAAGCAGTGGTATCAACGC	373	0.3579963720474897	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	367	0.3522377172692459	No Hit
GTTGATACCACTGCTTCCCATGTAC	366	0.35127794147287195	No Hit
TACCACTGCTTCCCATGTACTCTGC	355	0.3407204077127583	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	352	0.33784108032363636	No Hit
GCAGTGGTATCAACGCAGAGTACAT	350	0.3359215287308885	No Hit
CCACTGCTTCCCATGTACTCTGCGT	332	0.31864556439615704	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	324	0.3109673580251653	No Hit
CTTCCCATGTACTCTGCGTTGATAC	305	0.29273161789405994	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	266	0.2553003618354752	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	266	0.2553003618354752	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	261	0.2505014828536054	No Hit
CCATGTACTCTGCGTTGATACCACT	243	0.233225518518874	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	239	0.2293864153333781	No Hit
TTCCCATGTACTCTGCGTTGATACC	237	0.2274668637406302	No Hit
GGTATCAACGCAGAGTACATGGGGG	208	0.19963336564578515	No Hit
ATCAACGCAGAGTACATGGGAAGCA	185	0.1775585223291839	No Hit
TGATACCACTGCTTCCCATGTACTC	183	0.17563897073643597	No Hit
GTGGTATCAACGCAGAGTACTTTTT	174	0.16700098856907028	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	168	0.16124233379082648	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	156	0.14972502423433884	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	154	0.14780547264159094	No Hit
GTACATGGTAAGCAGTGGTATCAAC	145	0.13916749047422522	No Hit
CTCTGCGTTGATACCACTGCTTCCC	136	0.1305295083068595	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	135	0.12956973251048556	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	130	0.12477085352861571	No Hit
AAAAAAAACAAAAAAAAAAAAAAAA	125	0.11997197454674588	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	123	0.11805242295399795	No Hit
ACCATGTACTCTGCGTTGATACCAC	122	0.11709264715762398	No Hit
GTACTGGTTCACTATCGGTCAGTCA	114	0.10941444078663223	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	114	0.10941444078663223	No Hit
AAAAAGTACTCTGCGTTGATACCAC	109	0.10461556180476242	No Hit
TCAACGCAGAGTACATGGGAAGCAG	107	0.10269601021201448	No Hit
GGTATCAACGCAGAGTACATGGGCA	105	0.10077645861926654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	120	3.6379788E-12	14.250723	1
CACAAAA	260	0.0	9.865885	2
ACACAAA	240	1.8189894E-12	9.500482	1
CCATGTA	685	0.0	7.3507376	2
GAAAAAA	365	3.6379788E-12	7.2880406	1
TGATACC	745	0.0	6.8829412	17
TGCGTTG	760	0.0	6.8720393	12
TTGATAC	765	0.0	6.827125	16
TACTCTG	775	0.0	6.7390327	7
AGTGGTA	865	0.0	6.6965375	14
CTGCGTT	775	0.0	6.616505	11
GAAGCAG	835	0.0	6.5959606	9
CCCATGT	725	0.0	6.5520563	1
CAGTGGT	885	0.0	6.545203	13
CTCTGCG	785	0.0	6.5322175	9
ACTCTGC	800	0.0	6.528438	8
GGAAGCA	845	0.0	6.5179024	8
ATGTACT	775	0.0	6.5002327	4
TCTGCGT	790	0.0	6.4908743	10
TGTACTC	785	0.0	6.417427	5
>>END_MODULE