FastQCFastQC Report
Wed 25 May 2016
SRR1294852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104191
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46014.415928439116622No Hit
GGTATCAACGCAGAGTACTTTTTTT39563.7968730504554133No Hit
TATCAACGCAGAGTACTTTTTTTTT32433.112552907640775No Hit
GTACATGGGAAGCAGTGGTATCAAC26962.5875555470242153No Hit
CCCATGTACTCTGCGTTGATACCAC26462.5395667572055167No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17251.6556132487450932No Hit
GAGTACATGGGAAGCAGTGGTATCA15311.4694167442485435No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14511.3926346805386263No Hit
CATGTACTCTGCGTTGATACCACTG12241.1747655747617356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA10861.0423165148621283No Hit
GCGTTGATACCACTGCTTCCCATGT10150.9741724333195766No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10100.9693735543377068No Hit
ACGCAGAGTACATGGGAAGCAGTGG9960.9559366931884712No Hit
GGTATCAACGCAGAGTACATGGGAA9280.8906719390350414No Hit
TATCAACGCAGAGTACATGGGAAGC8770.8417233734199691No Hit
ACTCTGCGTTGATACCACTGCTTCC8460.8119703237323762No Hit
GTATCAACGCAGAGTACATGGGAAG8140.7812574982484092No Hit
GTGGTATCAACGCAGAGTACATGGG7810.7495848969680683No Hit
GTGGTATCAACGCAGAGTACTTTTT6820.6545670931270455No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6780.6507279899415497No Hit
GCTTCCCATGTACTCTGCGTTGATA5450.523077809023812No Hit
ATCAACGCAGAGTACTTTTTTTTTT5440.522118033227438No Hit
GTACTCTGCGTTGATACCACTGCTT5050.4846867771688534No Hit
GATACCACTGCTTCCCATGTACTCT4990.47892812239060956No Hit
CAGTGGTATCAACGCAGAGTACATG4770.45781305487038226No Hit
CATGGGAAGCAGTGGTATCAACGCA4630.44437619372114673No Hit
ATACCACTGCTTCCCATGTACTCTG4610.4424566421283988No Hit
GGGAAGCAGTGGTATCAACGCAGAG4400.4223013504045455No Hit
ACATGGGAAGCAGTGGTATCAACGC4260.40886448925531No Hit
CTGCTTCCCATGTACTCTGCGTTGA4080.39158852492057855No Hit
GCAGTGGTATCAACGCAGAGTACAT4040.3877494217350827No Hit
GCAGAGTACATGGGAAGCAGTGGTA3810.36567457841848144No Hit
GTTGATACCACTGCTTCCCATGTAC3630.34839861408375No Hit
TACCACTGCTTCCCATGTACTCTGC3590.3445595108982542No Hit
CTTCCCATGTACTCTGCGTTGATAC3480.33400197713814056No Hit
CCACTGCTTCCCATGTACTCTGCGT3360.32248466758165295No Hit
AAGCAGTGGTATCAACGCAGAGTAC2790.2677774471883368No Hit
AACGCAGAGTACTTTTTTTTTTTTT2790.2677774471883368No Hit
ATGGGAAGCAGTGGTATCAACGCAG2760.2648981197992149No Hit
CCATGTACTCTGCGTTGATACCACT2610.2505014828536054No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2550.24474282807536157No Hit
CAACGCAGAGTACTTTTTTTTTTTT2540.24378305227898764No Hit
TGATACCACTGCTTCCCATGTACTC2310.22170820896238638No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2060.19771381405303723No Hit
GGTATCAACGCAGAGTACATGGGGG2040.19579426246028928No Hit
ATCAACGCAGAGTACATGGGAAGCA2040.19579426246028928No Hit
TTCCCATGTACTCTGCGTTGATACC2040.19579426246028928No Hit
TCAACGCAGAGTACTTTTTTTTTTT2000.1919551592747934No Hit
GTACATGGTAAGCAGTGGTATCAAC1450.13916749047422522No Hit
GGAAGCAGTGGTATCAACGCAGAGT1380.13244905989960745No Hit
TGGGAAGCAGTGGTATCAACGCAGA1340.1286099567141116No Hit
GTACTGGTTCACTATCGGTCAGTCA1330.1276501809177376No Hit
AAAAAGTACTCTGCGTTGATACCAC1300.12477085352861571No Hit
CTGCGTTGATACCACTGCTTCCCAT1290.12381107773224176No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1280.12285130193586777No Hit
CTCTGCGTTGATACCACTGCTTCCC1200.11517309556487605No Hit
ACCATGTACTCTGCGTTGATACCAC1170.11229376817575414No Hit
GGTATCAACGCAGAGTACATGGGGA1150.11037421658300621No Hit
TCAACGCAGAGTACATGGGAAGCAG1100.10557533760113637No Hit
AACGCAGAGTACATGGGAAGCAGTG1060.10173623441564052No Hit
AAAGTACTCTGCGTTGATACCACTG1050.10077645861926654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCCG250.00606035318.96447418
GAACAAA402.6401045E-416.6981281
AACAAAA1054.233524E-49.0830282
GGTATCA15050.07.5446691
CCATGTA8350.07.19571732
TACTCTG9150.06.73925077
CCCATGT8500.06.73537971
TGCGTTG9200.06.702624312
CTGCGTT9250.06.66639411
TGTACTC9050.06.63914165
ATGTACT9200.06.5308954
GTACTCT9350.06.49987656
CTCTGCG9600.06.42026479
TCTGCGT9600.06.420264710
GAAGCAG7250.06.40868439
CGTTGAT9700.06.35712814
ACTCTGC9700.06.35407648
TTGATAC9900.06.324527716
TGATACC9800.06.292259717
CATGTAC9700.06.1942513
Produced by FastQC (version 0.11.5)