FastQCFastQC Report
Wed 25 May 2016
SRR1294851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181673
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC142260.340198767335466No Hit
CCCATGTACTCTGCGTTGATACCAC130920.31308043455334744No Hit
GAGTACATGGGAAGCAGTGGTATCA62700.14993998813393586No Hit
CATGTACTCTGCGTTGATACCACTG47970.11471485216562845No Hit
GCGTTGATACCACTGCTTCCCATGT43290.10352315927142079No Hit
ATTCCATTCCATTCCATTCCATTCC42840.10244703495467006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG852.7109672E-713.4083999
TAGCCCG1251.8300852E-811.4020575
TGGACCG1451.4419129E-810.484655
GCGCGTC1002.4076928E-510.4473769
CGGACTG1106.038941E-610.3655065
CCGTACA750.00264678810.1344332
CGAGCGC750.002654956710.13066910
CGCGTCC1356.474238E-79.84926110
TGGACGG1856.475602E-109.7585175
CTAGACT4100.09.7336964
GTATAGA5500.09.6740081
CTACACT5600.09.5019424
CGTACAC1106.834895E-59.50091653
CGTCTCG900.00111690289.4976168
CGCGCGT1002.7642972E-49.4976158
TCGTCGA800.00453153539.49659213
CGTCGAG1002.7732903E-49.49454414
TAGACAG4850.09.4037595
TAGACTG5400.09.3257565
GCGGGAC1951.6825652E-99.2573033
Produced by FastQC (version 0.11.5)