FastQCFastQC Report
Wed 25 May 2016
SRR1294851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181673
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC140820.3367551695218636No Hit
CCCATGTACTCTGCGTTGATACCAC135630.3243438690686718No Hit
GTATCAACGCAGAGTACTTTTTTTT79490.1900913820855911No Hit
GAGTACATGGGAAGCAGTGGTATCA63580.1520444090200262No Hit
GGTATCAACGCAGAGTACTTTTTTT50440.12062157897090471No Hit
CATGTACTCTGCGTTGATACCACTG49810.11911500492745367No Hit
TATCAACGCAGAGTACTTTTTTTTT47230.11294522551141613No Hit
GCGTTGATACCACTGCTTCCCATGT43120.10311662341842608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC802.9053535E-511.86042710
GCGCGCC1052.767647E-711.74747111
CGCGCGC855.3946642E-511.16275510
TCGTCCG855.3946642E-511.1627559
CTAGGAC4600.010.9804053
GACTGCG1502.4940164E-810.1213837
GTATAGG5550.09.6190231
AGACCGT1752.7092028E-89.2211066
GGCGTCG1451.7369202E-69.16115813
TTGCGTG1451.7369202E-69.16115816
GCTCGTT2600.09.123404511
TGGACCG1151.0737576E-49.1158085
TACACGG1054.3552616E-49.0763455
GACGGAT950.00184449838.9893867
TAGACAG5650.08.9397985
GTCTTAG3850.08.9141041
TAGACTC2701.8189894E-128.82422355
GTTCTAG4550.08.7998211
TACACTG10700.08.728565
CGGGACT1752.5043119E-78.7132914
Produced by FastQC (version 0.11.5)