FastQCFastQC Report
Wed 25 May 2016
SRR1294850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307498
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC802.0051284E-613.0603038
TCGTCCG957.4127456E-812.9978149
GGTATCA16950.011.7145551
GCATCGT1604.5474735E-1111.2771326
CTAGTAC1701.0913936E-1111.1762843
AACAGCG1451.2241799E-911.1357217
CGTCCGC1451.4491889E-810.48099510
CGGGACT1752.3646862E-1010.3155194
CCCGCAT1201.5255046E-610.2914953
ATTATAC2650.010.0375683
TAGACGT951.6431097E-410.0011974
TCGTCTA951.6531849E-49.99513814
GTCGTAC1002.7553109E-49.5005621
TCGGACG1002.7616054E-49.4984039
GTCCGCA1606.670962E-89.49739511
GTATCAA33200.09.3002481
CCTATAC4000.09.2623453
ATCGTCC1651.0695294E-79.2105728
GTCTAGA2900.09.17295551
TCTAGAC2800.09.1605613
Produced by FastQC (version 0.11.5)