FastQCFastQC Report
Wed 25 May 2016
SRR1294850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307498
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62120.18781568424228828No Hit
GGTATCAACGCAGAGTACTTTTTTT37290.11274383234698858No Hit
TATCAACGCAGAGTACTTTTTTTTT35190.10639462215850169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA402.698506E-416.6759972
GGTATCA19650.011.8841851
ACCGGAC1053.3487067E-610.889293
TCGGCAC700.001455816410.889293
TTAGTAC1951.8189894E-1210.7496833
ACACCGT1158.919287E-710.7303696
TCGTCTA1201.5475234E-610.28000514
TCGTTCA1856.184564E-1110.25863413
CGAATGT750.002673948610.12185215
CTACGCT856.4202165E-410.0886074
GTATCAA39500.09.8211691
AAGACCG1002.6762794E-49.5281295
AATCCGA800.00456016569.48923611
CGGGACT2355.456968E-129.3254034
CTGCTCG3700.09.2323519
CTAGACA3200.09.2303744
CCTACAC4250.09.1918413
TTATACT3450.09.1138624
GTCTAAG2201.5825208E-109.0983471
TAGCCCG1054.363948E-49.0744095
Produced by FastQC (version 0.11.5)