FastQCFastQC Report
Wed 25 May 2016
SRR1294849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971502
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC190240.47901272616758095No Hit
CCCATGTACTCTGCGTTGATACCAC171720.43238049483545515No Hit
GAGTACATGGGAAGCAGTGGTATCA82720.20828391877934344No Hit
CATGTACTCTGCGTTGATACCACTG61710.1553820191957602No Hit
GCGTTGATACCACTGCTTCCCATGT61240.15419858783905938No Hit
GTATCAACGCAGAGTACATGGGAAG53190.1339291784317369No Hit
GGTATCAACGCAGAGTACATGGGGG45980.11577483783213505No Hit
ACGCAGAGTACATGGGAAGCAGTGG43300.10902676115988358No Hit
TATCAACGCAGAGTACATGGGAAGC41660.1048973411067148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT752.0738765E-411.4008781
AGCGTTA1751.8189894E-1211.39785710
TCGACTG700.001491013210.85825355
GTCTTAG1951.6370905E-109.7443411
CGTAGCA1701.6652848E-89.5007312
TATACCG1252.753056E-59.1209335
GTCCTAT2201.6734703E-109.068881
TTAGGCA3250.09.0626954
CGATAAC1054.5186075E-49.04295115
TAGCGAG950.00182809028.9981959
GTGTTAG2750.08.9825111
GTATTAG5200.08.9526131
CGAAACT1502.7238166E-68.861979516
ACGTCGT1401.0846714E-58.822335
ACCGTTC1401.0888316E-58.8196588
GTATAAA5750.08.7571961
TAATCGG1201.7751547E-48.7037316
GTACCGT1107.1789743E-48.6326696
ACGAAAC1554.240439E-68.57621815
GTGTAAA4000.08.5506591
Produced by FastQC (version 0.11.5)