FastQCFastQC Report
Wed 25 May 2016
SRR1294849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971502
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC186120.4686388172535227No Hit
GTACATGGGAAGCAGTGGTATCAAC185680.46753092406852625No Hit
GTATCAACGCAGAGTACTTTTTTTT115800.29157734277862635No Hit
GAGTACATGGGAAGCAGTGGTATCA83340.20984504099456577No Hit
GGTATCAACGCAGAGTACTTTTTTT69250.17436727968410942No Hit
TATCAACGCAGAGTACTTTTTTTTT67500.1699608863346915No Hit
CATGTACTCTGCGTTGATACCACTG65650.16530269907959255No Hit
GCGTTGATACCACTGCTTCCCATGT62790.15810139337711526No Hit
GTATCAACGCAGAGTACATGGGAAG54880.13818449543774622No Hit
ACGCAGAGTACATGGGAAGCAGTGG45550.11469212403770664No Hit
GGTATCAACGCAGAGTACATGGGGG44030.11086485667135507No Hit
TATCAACGCAGAGTACATGGGAAGC43850.11041162764112923No Hit
ACTCTGCGTTGATACCACTGCTTCC40000.10071756227240979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG855.1437753E-511.2150335
GTATCGT951.3734516E-510.9913726
GATACCG700.001450013810.8946035
GTCGTTA750.002679542210.11939412
ACGTCGT951.5890764E-410.0345035
CCTATAC3050.010.0016033
GTTGCGT1151.0443713E-59.89940713
TCCTATA1752.4265319E-99.8058872
TTAGGCT2159.094947E-129.7544714
ATAAGAC2106.184564E-119.5327793
TTAGACT1606.3007974E-89.5327784
AAAACCG1401.0111726E-69.5327785
CGGACTG1304.0608284E-69.5327785
CCTCGCG1502.7041642E-79.4871719
TTCGCGG1106.9456844E-59.48693214
TATCGTC800.00456964889.48693213
GTGTTAC1951.5734258E-99.2916391
ATTAGAC1451.6300437E-69.20406153
GTACTAA1803.972127E-89.006371
TCTATAC3600.09.003183
Produced by FastQC (version 0.11.5)