FastQCFastQC Report
Wed 25 May 2016
SRR1294848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263994
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1931208.53005794184967No Hit
CCCATGTACTCTGCGTTGATACCAC1695707.489860838853814No Hit
GAGTACATGGGAAGCAGTGGTATCA849603.752660121890782No Hit
CATGTACTCTGCGTTGATACCACTG611882.7026573391978954No Hit
GCGTTGATACCACTGCTTCCCATGT600342.65168547266468No Hit
GTATCAACGCAGAGTACATGGGAAG545062.407515214262935No Hit
ACGCAGAGTACATGGGAAGCAGTGG469142.072178636515821No Hit
TATCAACGCAGAGTACATGGGAAGC463762.048415322655449No Hit
ACTCTGCGTTGATACCACTGCTTCC405241.7899340722634425No Hit
GGTATCAACGCAGAGTACATGGGAA378051.6698365808389952No Hit
GCTTCCCATGTACTCTGCGTTGATA267581.1818935915907904No Hit
GTGGTATCAACGCAGAGTACATGGG240891.0640045865845933No Hit
GTACTCTGCGTTGATACCACTGCTT236411.0442165482770713No Hit
ATACCACTGCTTCCCATGTACTCTG229441.0134302476066632No Hit
CAGTGGTATCAACGCAGAGTACATG223820.9886068602655307No Hit
GGGAAGCAGTGGTATCAACGCAGAG202610.8949228664033563No Hit
CATGGGAAGCAGTGGTATCAACGCA185130.8177141812213283No Hit
GCAGTGGTATCAACGCAGAGTACAT184690.8157707131732681No Hit
CTGCTTCCCATGTACTCTGCGTTGA181130.8000462898753267No Hit
TACCACTGCTTCCCATGTACTCTGC178180.7870162200076503No Hit
GCAGAGTACATGGGAAGCAGTGGTA170910.7549048274862918No Hit
GATACCACTGCTTCCCATGTACTCT168770.7454525056161809No Hit
ACATGGGAAGCAGTGGTATCAACGC154160.6809205324749094No Hit
GTTGATACCACTGCTTCCCATGTAC153190.6766360688235039No Hit
GTATCAACGCAGAGTACTTTTTTTT146910.6488974794102811No Hit
CCATGTACTCTGCGTTGATACCACT143400.6333939047541646No Hit
CTTCCCATGTACTCTGCGTTGATAC140510.6206288532566783No Hit
CCACTGCTTCCCATGTACTCTGCGT139590.6165652382470979No Hit
AAGCAGTGGTATCAACGCAGAGTAC135700.599383213913111No Hit
ATGGGAAGCAGTGGTATCAACGCAG125940.5562735590288667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA108060.47729808471223867No Hit
TGATACCACTGCTTCCCATGTACTC106870.47204188703680305No Hit
TTCCCATGTACTCTGCGTTGATACC102950.45472735351772137No Hit
ATCAACGCAGAGTACATGGGAAGCA101470.4481902337197007No Hit
GGTATCAACGCAGAGTACTTTTTTT93390.41250109320077705No Hit
TATCAACGCAGAGTACTTTTTTTTT88080.3890469674389596No Hit
GTACATGGTAAGCAGTGGTATCAAC80820.3569797446459664No Hit
ACCATGTACTCTGCGTTGATACCAC63300.27959438055047847No Hit
GGAAGCAGTGGTATCAACGCAGAGT62540.2762374811947381No Hit
AAAAAGTACTCTGCGTTGATACCAC58570.25870209903383135No Hit
CTGTACTTGTTCGCTATCGGTCTCT54890.2424476389955097No Hit
CTGCGTTGATACCACTGCTTCCCAT50630.2236313347120178No Hit
TCCATGTACTCTGCGTTGATACCAC49400.21819845812312225No Hit
TCACTGTACTTGTTCGCTATCGGTC48890.215945801976507No Hit
CTCTGCGTTGATACCACTGCTTCCC46560.20565425526746095No Hit
GTACTAGGGGTTGCCCCATTCGGAT46010.2032249202073857No Hit
TCCCATGTACTCTGCGTTGATACCA44280.19558355720023993No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA43210.19085739626518444No Hit
TCAACGCAGAGTACATGGGAAGCAG41830.18476197375081382No Hit
AGCTAATACGGCTGATGGTGGATAC41120.1816259230368985No Hit
AAAGTACTCTGCGTTGATACCACTG40300.17800400531096813No Hit
GTACATGGAAAGCAGTGGTATCAAC40120.17720895020039806No Hit
TGGGAAGCAGTGGTATCAACGCAGA39870.17610470699127295No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38830.17151105524131247No Hit
GTACTTGTTCGCTATCGGTCTCTCG38150.16850751371249217No Hit
GGTAAGCAGTGGTATCAACGCAGAG36280.16024777450823632No Hit
CCCCATGTACTCTGCGTTGATACCA35950.15879017347219118No Hit
AGTGGTATCAACGCAGAGTACATGG35560.157067554065956No Hit
TTCCATCACTGTACTTGTTCGCTAT34820.15379899416694567No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34220.15114881046504539No Hit
TTGATACCACTGCTTCCCATGTACT31810.14050390592907933No Hit
GAGTACATGGTAAGCAGTGGTATCA31730.14015054810215927No Hit
GAGTACATGGAAAGCAGTGGTATCA29080.1284455700854331No Hit
ACTGCTTCCCATGTACTCTGCGTTG28970.12795970307341803No Hit
GTACTGGTTCACTATCGGTCACACA28820.12729715714794296No Hit
AACGCAGAGTACATGGGAAGCAGTG28630.12645793230900787No Hit
GTACATGGGTCAAGCTAATACGGCT27680.12226180811433246No Hit
GTACATGGGTAAGCAGTGGTATCAA27260.12040667952300227No Hit
GTAGTATTTAGCCTTACGAGATGGT26480.11696144071053191No Hit
CAACGCAGAGTACATGGGAAGCAGT25170.11117520629471632No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24340.10750911884042096No Hit
GCTTACCATGTACTCTGCGTTGATA23280.10282712763373048No Hit
CGCAGAGTACATGGGAAGCAGTGGT23150.10225292116498542No Hit
CGTTGATACCACTGCTTCCCATGTA23040.10176705415297037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGC250.00604070718.995757
GTACAAA4800.018.6065961
GGACACA402.759064E-416.627171
TTATATT350.002166794616.2900034
GTAAAAA3850.016.0410561
TACAAGA901.3460522E-1015.8354012
TCCGTGC551.13472015E-515.536818514
TACAAAA7450.015.4315432
TGCTTGG508.758141E-515.19155614
CCCCTAT456.757317E-414.7797071
ACGCAAA3200.014.5487741
GAACAAA21100.014.4544931
TTCAGTG1250.014.43357618
TCTTAGT400.005136802314.30920819
TTACTCG400.005270865714.2537544
GACCAAA1400.014.251861
TTCGTGT400.00528947914.2461828
AACTCAA400.005291032614.24555113
GAAGTAT400.00529958414.24208415
TCAGTGA1550.014.15534719
Produced by FastQC (version 0.11.5)