FastQCFastQC Report
Wed 25 May 2016
SRR1294848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263994
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1920188.481382901191434No Hit
CCCATGTACTCTGCGTTGATACCAC1784527.882176366191783No Hit
GAGTACATGGGAAGCAGTGGTATCA873073.856326474363448No Hit
CATGTACTCTGCGTTGATACCACTG637792.817101105391622No Hit
GCGTTGATACCACTGCTTCCCATGT622322.74877053561096No Hit
GTATCAACGCAGAGTACATGGGAAG549302.426243179089697No Hit
ACGCAGAGTACATGGGAAGCAGTGG509472.2503151510118844No Hit
TATCAACGCAGAGTACATGGGAAGC491732.1719580528923665No Hit
GTATCAACGCAGAGTACTTTTTTTT459552.0298198670137815No Hit
ACTCTGCGTTGATACCACTGCTTCC421081.8598989219936097No Hit
GGTATCAACGCAGAGTACATGGGAA382951.6914797477378472No Hit
TATCAACGCAGAGTACTTTTTTTTT291731.2885634855922763No Hit
GGTATCAACGCAGAGTACTTTTTTT290061.2811871409553206No Hit
GCTTCCCATGTACTCTGCGTTGATA283131.2505775191983723No Hit
GTGGTATCAACGCAGAGTACATGGG242571.0714251009499143No Hit
CAGTGGTATCAACGCAGAGTACATG240031.060205989945203No Hit
GTACTCTGCGTTGATACCACTGCTT238661.0541547371591975No Hit
ATACCACTGCTTCCCATGTACTCTG237281.048059314644827No Hit
GGGAAGCAGTGGTATCAACGCAGAG213890.9447463199990813No Hit
CATGGGAAGCAGTGGTATCAACGCA203810.9002232338071567No Hit
GCAGTGGTATCAACGCAGAGTACAT198970.8788450852784945No Hit
TACCACTGCTTCCCATGTACTCTGC185760.8204968741083236No Hit
CTGCTTCCCATGTACTCTGCGTTGA184760.8160799012718231No Hit
GCAGAGTACATGGGAAGCAGTGGTA184510.814975658062698No Hit
GATACCACTGCTTCCCATGTACTCT176630.7801699121110746No Hit
ACATGGGAAGCAGTGGTATCAACGC169840.7501786665512364No Hit
GTACTTTTTTTTTTTTTTTTTTTTT159060.7025636993737616No Hit
GTTGATACCACTGCTTCCCATGTAC154370.6818480967705745No Hit
ACGCAGAGTACTTTTTTTTTTTTTT152030.6715123803331635No Hit
CCATGTACTCTGCGTTGATACCACT151450.6689505360879932No Hit
CCACTGCTTCCCATGTACTCTGCGT148820.657333897527997No Hit
AAGCAGTGGTATCAACGCAGAGTAC146270.6460706167949208No Hit
CTTCCCATGTACTCTGCGTTGATAC142560.6296836475715041No Hit
ATGGGAAGCAGTGGTATCAACGCAG133570.5899750617713652No Hit
ATCAACGCAGAGTACATGGGAAGCA110060.4861320303852395No Hit
TGATACCACTGCTTCCCATGTACTC107260.47376450644303825No Hit
GAGTACTTTTTTTTTTTTTTTTTTT105440.46572561588060746No Hit
TTCCCATGTACTCTGCGTTGATACC105220.4647538818565774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA98560.43533684276548434No Hit
GTACATGGTAAGCAGTGGTATCAAC81150.3584373456820115No Hit
GGAAGCAGTGGTATCAACGCAGAGT67840.29964743722819054No Hit
ACCATGTACTCTGCGTTGATACCAC64270.2838788442018839No Hit
GCAGAGTACTTTTTTTTTTTTTTTT61730.2726597331971728No Hit
AAAAAGTACTCTGCGTTGATACCAC60320.2664318014977071No Hit
CTGTACTTGTTCGCTATCGGTCTCT57220.2527391857045558No Hit
TCACTGTACTTGTTCGCTATCGGTC51800.22879919293072332No Hit
CTGCGTTGATACCACTGCTTCCCAT51420.22712074325285317No Hit
TCCATGTACTCTGCGTTGATACCAC50810.2244263898225879No Hit
CTCTGCGTTGATACCACTGCTTCCC50120.22137867856540255No Hit
TCCCATGTACTCTGCGTTGATACCA47530.20993871891886637No Hit
GTGGTATCAACGCAGAGTACTTTTT47510.2098503794621364No Hit
GTACTAGGGGTTGCCCCATTCGGAT45750.20207650726989562No Hit
ATCAACGCAGAGTACTTTTTTTTTT45250.19986802085164537No Hit
TCAACGCAGAGTACATGGGAAGCAG45090.1991613051978053No Hit
TGGGAAGCAGTGGTATCAACGCAGA44780.19779204361849018No Hit
AAAGTACTCTGCGTTGATACCACTG42370.18714713908252406No Hit
AGCTAATACGGCTGATGGTGGATAC40510.17893156960663323No Hit
CCCCATGTACTCTGCGTTGATACCA39780.1757071794359879No Hit
GTACATGGAAAGCAGTGGTATCAAC38860.1716435644264075No Hit
GTACTTGTTCGCTATCGGTCTCTCG38770.17124603687112247No Hit
AGTGGTATCAACGCAGAGTACATGG37240.16448806843127675No Hit
GGTAAGCAGTGGTATCAACGCAGAG37240.16448806843127675No Hit
TTCCATCACTGTACTTGTTCGCTAT34280.15141382883523544No Hit
ACTGCTTCCCATGTACTCTGCGTTG32270.14253571343386953No Hit
GAGTACATGGTAAGCAGTGGTATCA31530.1392671535348592No Hit
AACGCAGAGTACATGGGAAGCAGTG31280.13816291032573408No Hit
TTGATACCACTGCTTCCCATGTACT30860.1363077817344039No Hit
GTACTGGTTCACTATCGGTCACACA29440.13003568030657325No Hit
GTACATGGGTAAGCAGTGGTATCAA28880.127562175518133No Hit
GAGTACATGGAAAGCAGTGGTATCA28710.12681129013592793No Hit
GTAGTATTTAGCCTTACGAGATGGT27890.12318937240999754No Hit
CAACGCAGAGTACATGGGAAGCAGT27320.12067169789319229No Hit
GTACATGGGTCAAGCTAATACGGCT25760.11378122026825159No Hit
CGCAGAGTACATGGGAAGCAGTGGT25630.11320701379950654No Hit
GCTTACCATGTACTCTGCGTTGATA24780.10945258688848115No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA24090.10640487563129584No Hit
CGTTGATACCACTGCTTCCCATGTA23540.10397554057122059No Hit
GCGTTGATACCACTGCTTACCATGT23360.10318048546065052No Hit
GCTTTCCATGTACTCTGCGTTGATA23170.10234126062171542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGG250.0059198719.0623193
TATACAG350.002123643416.339135
GATAGTG3150.016.27270319
AAGTGTC350.00218803416.2662337
AGTGTCC350.002189002516.2651568
CGATAGT3300.016.1008618
TGAAGTG551.0942669E-515.5964425
GTACAAA755.5944838E-815.255271
GAACAAA7200.015.0963621
AAAGGTG456.829102E-414.75912318
CCGATAG3950.014.65236617
CGCAAAA1152.3646862E-1114.0901652
AACCGAT2050.013.4220615
TCCTCGT801.9283234E-613.1053445
ACTAGTC1550.012.9131843
ACGGCTG6800.012.8367168
CGGCTGA6800.012.8367169
TACTAGT1800.012.7087762
CTAGTCC1650.012.7082144
CAACAGA604.122534E-412.6559876
Produced by FastQC (version 0.11.5)