FastQCFastQC Report
Wed 25 May 2016
SRR1294847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707338
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC449296.3518431075384045No Hit
CCCATGTACTCTGCGTTGATACCAC412195.827341384175599No Hit
GAGTACATGGGAAGCAGTGGTATCA222393.1440414624974196No Hit
CATGTACTCTGCGTTGATACCACTG178712.526514905179702No Hit
GCGTTGATACCACTGCTTCCCATGT146612.072700745612423No Hit
ACGCAGAGTACATGGGAAGCAGTGG139421.9710520288744562No Hit
TATCAACGCAGAGTACATGGGAAGC122621.7335418145214876No Hit
GTATCAACGCAGAGTACATGGGAAG121261.7143147971691044No Hit
GGTATCAACGCAGAGTACATGGGAA120621.705266789003277No Hit
ACTCTGCGTTGATACCACTGCTTCC117561.6620059999604149No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA104661.479632085367957No Hit
GTGGTATCAACGCAGAGTACATGGG84621.1963163296754875No Hit
GTACTCTGCGTTGATACCACTGCTT77701.0984847413824792No Hit
GCTTCCCATGTACTCTGCGTTGATA75241.06370645999508No Hit
ATACCACTGCTTCCCATGTACTCTG65460.9254415852110306No Hit
GATACCACTGCTTCCCATGTACTCT62790.8876944261442196No Hit
GTATCAACGCAGAGTACTTTTTTTT61480.8691742844297917No Hit
CAGTGGTATCAACGCAGAGTACATG60670.8577228990949164No Hit
GGGAAGCAGTGGTATCAACGCAGAG58250.8235101182178817No Hit
CATGGGAAGCAGTGGTATCAACGCA55200.7803907043026107No Hit
CTGCTTCCCATGTACTCTGCGTTGA54530.77091857075401No Hit
GCAGAGTACATGGGAAGCAGTGGTA53780.7603154361846811No Hit
GCAGTGGTATCAACGCAGAGTACAT51170.7234165278834164No Hit
GTTGATACCACTGCTTCCCATGTAC50900.7195993994384581No Hit
TACCACTGCTTCCCATGTACTCTGC48310.6829832413923754No Hit
ACATGGGAAGCAGTGGTATCAACGC46820.6619183473813085No Hit
GGTATCAACGCAGAGTACTTTTTTT45510.6433982056668807No Hit
CCACTGCTTCCCATGTACTCTGCGT44110.6236056878041333No Hit
CTTCCCATGTACTCTGCGTTGATAC42110.5953306622859227No Hit
AAGCAGTGGTATCAACGCAGAGTAC40510.5727106418713543No Hit
ATGGGAAGCAGTGGTATCAACGCAG39160.5536249996465622No Hit
TATCAACGCAGAGTACTTTTTTTTT37770.5339738569114059No Hit
CCATGTACTCTGCGTTGATACCACT36620.5177157172384348No Hit
TGATACCACTGCTTCCCATGTACTC33050.467244796688429No Hit
TTCCCATGTACTCTGCGTTGATACC31930.4514107823982311No Hit
ATCAACGCAGAGTACATGGGAAGCA27010.3818542196234332No Hit
GTACATGGTAAGCAGTGGTATCAAC23570.33322117573211113No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA21090.29816014408953No Hit
GGAAGCAGTGGTATCAACGCAGAGT19100.2700264936989106No Hit
ACCATGTACTCTGCGTTGATACCAC18160.2567372317053516No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17590.24867884943266164No Hit
CTGCGTTGATACCACTGCTTCCCAT16130.2280380808043679No Hit
AAACAAAAAAAAAAAAAAAAAAAAA15920.22506920312495585No Hit
AAAAAGTACTCTGCGTTGATACCAC15350.21701082085226583No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15250.21559706957635527No Hit
CTCTGCGTTGATACCACTGCTTCCC15220.21517294419358216No Hit
TCAACGCAGAGTACATGGGAAGCAG13920.19679417760674528No Hit
TGGGAAGCAGTGGTATCAACGCAGA13220.1868979186753716No Hit
TCCATGTACTCTGCGTTGATACCAC12980.18350491561318633No Hit
AAAGTACTCTGCGTTGATACCACTG12450.17601203385086056No Hit
CTGTACTTGTTCGCTATCGGTCTCT11900.16823640183335264No Hit
AGTGGTATCAACGCAGAGTACATGG11510.1627227718573016No Hit
GGTAAGCAGTGGTATCAACGCAGAG11150.1576332672640237No Hit
TCCCATGTACTCTGCGTTGATACCA10950.15480576471220267No Hit
TCACTGTACTTGTTCGCTATCGGTC10740.15183688703279055No Hit
CCCCATGTACTCTGCGTTGATACCA10690.15113001139483528No Hit
AACGCAGAGTACATGGGAAGCAGTG10650.15056451088447106No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10530.14886800935337846No Hit
GAACAAAAAAAAAAAAAAAAAAAAA9810.13868900016682265No Hit
GTACTAGGGGTTGCCCCATTCGGAT9520.13458912146668214No Hit
ACTGCTTCCCATGTACTCTGCGTTG9360.13232711942522526No Hit
GTACATGGGTAAGCAGTGGTATCAA9210.1302064925113595No Hit
GTACTTGTTCGCTATCGGTCTCTCG9200.13006511738376844No Hit
GAGTACATGGTAAGCAGTGGTATCA9200.13006511738376844No Hit
GTACATGGAAAGCAGTGGTATCAAC8990.12709623970435635No Hit
ATGTACTCTGCGTTGATACCACTGC8980.12695486457676528No Hit
TTGATACCACTGCTTCCCATGTACT8940.12638936406640106No Hit
CAACGCAGAGTACATGGGAAGCAGT8210.11606897975225422No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8070.11408972796597949No Hit
GTGGTATCAACGCAGAGTACTTTTT7970.11267597669006896No Hit
AGCTAATACGGCTGATGGTGGATAC7880.11140360054174948No Hit
GCTTACCATGTACTCTGCGTTGATA7870.11126222541415842No Hit
GAGTACATGGAAAGCAGTGGTATCA7620.10772784722438211No Hit
TTCCATCACTGTACTTGTTCGCTAT7260.10263834263110423No Hit
GTACTGGTTCACTATCGGTCACACA7200.1017900918655579No Hit
CGCAGAGTACATGGGAAGCAGTGGT7160.1012245913551937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA1200.018.2080631
GTCCCGC402.7662178E-416.61886412
GCACAAA350.002168837216.2854711
TAACAAA601.4642701E-615.8330971
GACCAAA551.1267266E-515.5452221
GAACAAA7150.014.8808971
CCGCCCT456.7747984E-414.77232315
TGTCCCG456.7747984E-414.77232311
GCTAAAA653.3663455E-614.6151671
CGCAAAA851.711669E-814.5291952
CTAGTGC400.00513604514.30757219
GTAAAAA602.5647032E-514.2497881
GTCCCTG400.005288262414.244740511
TCCTGAA707.2502316E-613.5712262
GGAAACA852.69245E-713.4115651
CCCTACT500.001501504413.29603318
TCCCGCC500.001502306213.29509313
CCCGCCC500.001502306213.29509314
TACAAAA2500.012.9198072
ATCCTGA853.935358E-612.2939351
Produced by FastQC (version 0.11.5)