##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294847_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	707338
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.913291806745857	33.0	31.0	34.0	16.0	34.0
2	30.0592333509581	33.0	31.0	34.0	16.0	34.0
3	30.429992450568186	34.0	31.0	34.0	23.0	34.0
4	33.10485934588556	37.0	35.0	37.0	17.0	37.0
5	34.07365644147494	37.0	35.0	37.0	28.0	37.0
6	33.795727078143685	37.0	35.0	37.0	25.0	37.0
7	33.99513104060576	37.0	35.0	37.0	27.0	37.0
8	33.87983396905015	37.0	35.0	37.0	25.0	37.0
9	35.11919337007202	39.0	37.0	39.0	21.0	39.0
10	35.45153236500796	39.0	37.0	39.0	27.0	39.0
11	35.32290927392562	39.0	37.0	39.0	25.0	39.0
12	35.50809513980586	39.0	37.0	39.0	27.0	39.0
13	35.03023165728407	39.0	37.0	39.0	21.0	39.0
14	36.48435825588333	41.0	38.0	41.0	24.0	41.0
15	36.3304869807645	41.0	38.0	41.0	22.0	41.0
16	36.370079085246374	40.0	38.0	41.0	25.0	41.0
17	36.48613392748587	41.0	38.0	41.0	25.0	41.0
18	36.506975448795345	41.0	38.0	41.0	25.0	41.0
19	36.44786509419825	40.0	37.0	41.0	25.0	41.0
20	36.61108692025594	41.0	38.0	41.0	25.0	41.0
21	36.57292977331912	41.0	37.0	41.0	25.0	41.0
22	36.702898755616125	41.0	38.0	41.0	26.0	41.0
23	36.787709694658	41.0	38.0	41.0	26.0	41.0
24	36.96711049031722	41.0	38.0	41.0	27.0	41.0
25	36.12891008259135	40.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1849381420326317
1101	2	-0.022064406754203958
1101	3	-0.05799289213563341
1101	4	-0.2074558132262041
1101	5	-0.15917531242509142
1101	6	-0.08552490773482191
1101	7	-0.1412097271499988
1101	8	-0.11598079154810392
1101	9	-0.10635566444747724
1101	10	-0.15985153535058316
1101	11	-0.02388826222150442
1101	12	-0.07897755461494427
1101	13	-0.20370029974767334
1101	14	-0.048751353036166734
1101	15	-0.05643043544596793
1101	16	0.013185033570501048
1101	17	-0.042156497722743325
1101	18	-0.16603362264554278
1101	19	-0.252973219168517
1101	20	-0.5485830831923337
1101	21	-0.36783560033212837
1101	22	-0.5176104576582787
1101	23	-0.5046836989901351
1101	24	-0.47210421502094135
1101	25	-0.7828656348316869
1102	1	-0.013002704374450502
1102	2	0.15235202729644115
1102	3	-0.05965557213268369
1102	4	-0.008257633057681346
1102	5	-0.38605516100400195
1102	6	-0.17971594662763835
1102	7	-0.016285155518012573
1102	8	-0.16630399396528617
1102	9	-0.0344263044707489
1102	10	-0.10427982088005905
1102	11	0.16980093383147477
1102	12	-0.15424095698784157
1102	13	0.1603593309897704
1102	14	0.010171768634670286
1102	15	0.21299752449342435
1102	16	0.05469776848374153
1102	17	-0.17685311288829553
1102	18	-0.02401340133111063
1102	19	-0.26372854473688534
1102	20	-0.129218721848126
1102	21	-0.19764966669173134
1102	22	-0.2504594576626218
1102	23	-0.1616376518707341
1102	24	-0.027843065731381955
1102	25	0.054159166449842644
1103	1	-0.5482842024283272
1103	2	-0.4581335325838296
1103	3	-0.4940820026086392
1103	4	-0.8459020597946321
1103	5	-0.6105568823766063
1103	6	-0.7010466647005202
1103	7	-0.6169767693175956
1103	8	-0.41874813874446914
1103	9	-0.6345897582490849
1103	10	-0.6380503930704364
1103	11	-0.732930787438967
1103	12	-0.6509422554237929
1103	13	-0.8626181838998477
1103	14	-0.8284217116027435
1103	15	-0.8603768766017126
1103	16	-0.9890690238380131
1103	17	-0.8050376522040352
1103	18	-0.9175498259840253
1103	19	-0.737090686798652
1103	20	-0.7547951518132905
1103	21	-1.0405113337159548
1103	22	-0.9445792079869761
1103	23	-1.0067631537249184
1103	24	-0.8711123456153302
1103	25	-0.8082577019800823
1104	1	-0.11748435550907743
1104	2	-0.0252670955382861
1104	3	0.05589120826009264
1104	4	0.23985377640896388
1104	5	0.10967273874853589
1104	6	0.01863186572428077
1104	7	-0.056241981717136014
1104	8	-0.2367657430307304
1104	9	0.15832260302148882
1104	10	-0.13539444205628115
1104	11	-0.2245496163712275
1104	12	0.037216948556306306
1104	13	0.1509600786336378
1104	14	-0.046760785464208254
1104	15	-0.02345379967863437
1104	16	-0.22470622246029137
1104	17	-0.12998216043212807
1104	18	-0.1024254478745732
1104	19	-0.1798155241503565
1104	20	-0.04662829379568478
1104	21	-0.15801611748939592
1104	22	-0.1442424303367673
1104	23	-0.23817532364454763
1104	24	-0.08519577462413253
1104	25	-0.3870560180918332
1105	1	-0.21366055280183005
1105	2	-0.30249254067754094
1105	3	-0.09890022296620415
1105	4	-0.08102419641807757
1105	5	-0.10718052225751507
1105	6	-0.12066746374155457
1105	7	-0.06527786910757527
1105	8	0.010082244111089267
1105	9	-0.2776009510607338
1105	10	-0.04926269590494314
1105	11	-0.1819267478913602
1105	12	-0.17426199365721118
1105	13	-0.10479320497469047
1105	14	-0.0975013866847334
1105	15	-0.0625969223085363
1105	16	-0.3034686223196488
1105	17	-0.2612200117091561
1105	18	-0.4884913962911597
1105	19	-0.21640810558132983
1105	20	-0.22255235015069275
1105	21	-0.36199110430473525
1105	22	-0.45379415187673544
1105	23	-0.38234167639072325
1105	24	-0.5050104359667031
1105	25	-0.7457290133955823
1106	1	0.2043015018627159
1106	2	0.29109007593607217
1106	3	-0.09099781417580388
1106	4	0.1806400344217849
1106	5	0.12149866022289757
1106	6	0.11565109649350802
1106	7	0.08049680033414575
1106	8	0.00653754222567926
1106	9	-0.06322587454261708
1106	10	-0.009394842697304284
1106	11	0.10867854869287186
1106	12	0.01819351105630318
1106	13	0.12890078376184277
1106	14	0.06343152222810033
1106	15	0.21983145673161886
1106	16	0.24322847305253248
1106	17	0.14537240687556618
1106	18	0.23810840629209196
1106	19	0.1354509021316943
1106	20	-0.08011387071876186
1106	21	0.0026779930875306945
1106	22	-0.021865627452150704
1106	23	0.14674955616314378
1106	24	0.101117326337409
1106	25	0.2795104883184223
1107	1	-0.48626552867381534
1107	2	-0.6838269831392694
1107	3	-0.3896725430570811
1107	4	-0.4685831747677156
1107	5	-0.49975472876111837
1107	6	-0.37781351537864083
1107	7	-0.4298984622229298
1107	8	-0.4847741729569677
1107	9	-0.6138305406385101
1107	10	-0.5031359243183076
1107	11	-0.574953550336808
1107	12	-0.60881044870397
1107	13	-0.5509436797084746
1107	14	-0.4570593453518086
1107	15	-0.4877741368756858
1107	16	-0.34044910971002196
1107	17	-0.33338223068150796
1107	18	-0.2739322971896456
1107	19	-0.2969352993523273
1107	20	-0.23916780907492807
1107	21	-0.5339346282024238
1107	22	-0.4084925181484991
1107	23	-0.6333562158117445
1107	24	-0.33668857406213704
1107	25	-0.5626092320012148
1108	1	0.030751494662254686
1108	2	0.25849989016418107
1108	3	0.06665807580115413
1108	4	0.004514062359575632
1108	5	-0.004348293982168627
1108	6	0.15502321630917493
1108	7	0.3124452450346098
1108	8	-0.012131452589990488
1108	9	0.10128845437443346
1108	10	0.11011660603541173
1108	11	0.08630941706844908
1108	12	0.046249206620821326
1108	13	0.10484404413050896
1108	14	-0.10275511371659718
1108	15	0.12364770097124733
1108	16	0.0874195012783403
1108	17	0.08361585445621245
1108	18	0.28921817731743715
1108	19	0.2694388773621057
1108	20	0.1494440209863086
1108	21	0.18515297148614707
1108	22	0.2530776689188201
1108	23	0.26390195391660143
1108	24	0.23483258411391006
1108	25	0.5983864963829362
1109	1	-0.021402596224472603
1109	2	0.08669658727948715
1109	3	-0.15332404860640736
1109	4	-0.2544376735408491
1109	5	0.0803196955876686
1109	6	-0.057502818097241004
1109	7	-0.13344336870500229
1109	8	-0.040886556973610766
1109	9	-0.0811098269447541
1109	10	0.010921827676362739
1109	11	-0.09244814725908412
1109	12	-0.048619350376078785
1109	13	-0.3499971254908232
1109	14	-0.1583211468110477
1109	15	-0.22117165714738718
1109	16	-0.2770047898478225
1109	17	0.03745008703570818
1109	18	-0.010456290104706056
1109	19	0.07548787944486435
1109	20	-0.2023058191173419
1109	21	-0.11276837590713029
1109	22	0.05491442704073535
1109	23	0.11092952168805681
1109	24	-0.05883388029070602
1109	25	-0.26498739869225574
1110	1	0.16124708848552416
1110	2	0.12633884238343995
1110	3	0.08003425819261878
1110	4	0.1617701056937122
1110	5	0.23548920298605225
1110	6	0.10676951754614095
1110	7	-0.15752403299918427
1110	8	-0.14633389417782894
1110	9	-0.08757483287595136
1110	10	-0.08664950278502204
1110	11	0.045558345841996584
1110	12	0.016339755573845594
1110	13	-0.06356083904517362
1110	14	-0.08941935496488185
1110	15	0.06593891287197096
1110	16	0.16826273840340633
1110	17	-0.07679727514197765
1110	18	0.2058201661605139
1110	19	0.25848407099134363
1110	20	0.4034661731408846
1110	21	0.4266397529559498
1110	22	0.49359832539872883
1110	23	0.6053604388385807
1110	24	0.5664236585742515
1110	25	0.4474244137570196
1111	1	-0.01463981787748736
1111	2	0.16535973362346468
1111	3	0.031163999069512727
1111	4	0.061354671879193745
1111	5	0.20333193588303544
1111	6	0.2136600402754496
1111	7	-0.07810892794524449
1111	8	-0.04794033788505203
1111	9	-0.1434237823364839
1111	10	-0.03132507587447719
1111	11	-0.06048636559588516
1111	12	0.027438413632964398
1111	13	0.0855261031826231
1111	14	-0.10554207232045343
1111	15	0.09118473611832911
1111	16	0.014852594645375916
1111	17	0.22931019967795407
1111	18	0.28829573327249847
1111	19	0.27122802627990694
1111	20	0.2925304010018479
1111	21	0.23296540524681575
1111	22	0.2864403111125853
1111	23	0.413450937658034
1111	24	0.2593986428834043
1111	25	0.17599819061825173
1112	1	-0.20205700508172697
1112	2	-0.1504757430998751
1112	3	0.053520670301320905
1112	4	-0.14701902399324496
1112	5	-0.05298688920520789
1112	6	-0.09778844599015457
1112	7	-0.38677239901225846
1112	8	-0.26356815630373376
1112	9	-0.4130650289543851
1112	10	-0.33319233044240093
1112	11	-0.2255802850489559
1112	12	-0.36105102529990774
1112	13	-0.49247380774142613
1112	14	-0.2809312228699383
1112	15	-0.32214158248406477
1112	16	-0.37072934965988935
1112	17	-0.27097512171920357
1112	18	-0.2984711995085618
1112	19	-0.03350079312974685
1112	20	-0.2759369916337917
1112	21	-0.15297841009874702
1112	22	-0.031995069854112046
1112	23	0.13325001572196982
1112	24	0.15702393377205226
1112	25	0.022949009560250033
1113	1	0.20755803237652515
1113	2	0.2036016582370479
1113	3	0.08911331244889098
1113	4	0.2602366212922149
1113	5	0.05831403517212408
1113	6	0.33125324921487476
1113	7	0.23620735069970067
1113	8	0.19116735284712405
1113	9	-0.07093524446544563
1113	10	0.3883715184927823
1113	11	-0.04335025791760927
1113	12	0.16222101029472924
1113	13	0.06757488002504175
1113	14	0.02408343124515966
1113	15	0.257034442109358
1113	16	0.2166351926910366
1113	17	-0.05200428128446788
1113	18	0.29515917167592676
1113	19	0.2687695328181263
1113	20	0.2754088242446855
1113	21	0.18592189413342908
1113	22	0.19985055222900172
1113	23	0.27668314039344466
1113	24	0.23685634060426253
1113	25	0.16748583864335842
1114	1	0.26937496859108734
1114	2	0.21143134761378235
1114	3	0.20406715805724218
1114	4	0.032089122253040614
1114	5	0.2448279239115152
1114	6	0.2569459133290479
1114	7	0.40305014211123336
1114	8	0.1896091395561541
1114	9	0.014230830932831395
1114	10	0.21601831250534786
1114	11	0.18463958362077193
1114	12	0.12126994933572632
1114	13	0.10754580519598278
1114	14	-0.07584166561289862
1114	15	0.11878654246817177
1114	16	0.2827278763066232
1114	17	0.40151493171968156
1114	18	0.432269278028258
1114	19	0.4672888186359785
1114	20	0.27719687557898
1114	21	0.5198854353614948
1114	22	0.41402605098705436
1114	23	0.48147453545971786
1114	24	0.4468744933775497
1114	25	0.6020120715218482
1115	1	0.3589295322029997
1115	2	0.2475074207924841
1115	3	0.19640967746463645
1115	4	0.37164905168181406
1115	5	0.38059044941447695
1115	6	0.14803209570672493
1115	7	0.20362204620354163
1115	8	0.13899814165526436
1115	9	-0.10109437129714394
1115	10	-0.05472547556512808
1115	11	0.07691299130695484
1115	12	-0.0421850970770592
1115	13	0.05352337424177733
1115	14	0.08708415794800572
1115	15	0.14423891149164803
1115	16	0.24556269561350774
1115	17	0.1674868221981498
1115	18	0.327545703449843
1115	19	0.19505425333153426
1115	20	0.40038253958808667
1115	21	0.6047775640454347
1115	22	0.5967440733247997
1115	23	0.6389946818024015
1115	24	0.6173885018389811
1115	25	0.6498420431964718
1116	1	0.26024458221985824
1116	2	0.2417564185819998
1116	3	0.40951521865829577
1116	4	0.43108856312762356
1116	5	0.3618575431914053
1116	6	0.18559157268398252
1116	7	0.24393526039558822
1116	8	0.28249220332399005
1116	9	0.21296280153265457
1116	10	0.12885700542097567
1116	11	0.16879789633112097
1116	12	0.07866088096798052
1116	13	0.11052760787858773
1116	14	0.18449527030797697
1116	15	0.0853988530449854
1116	16	0.2033949237053747
1116	17	0.29421493279722455
1116	18	0.2730891450593731
1116	19	0.30261860086441317
1116	20	0.42947474295251453
1116	21	0.45504544460442986
1116	22	0.568438652021733
1116	23	0.5144395883252031
1116	24	0.41501691591113854
1116	25	0.46075208778079
1201	1	0.10094345169570573
1201	2	0.09395392325107821
1201	3	0.04758172397938765
1201	4	0.2693285702003081
1201	5	-0.22401477278069848
1201	6	0.040850222906662736
1201	7	0.08336325716867776
1201	8	0.04202098582793923
1201	9	0.11775233396200946
1201	10	0.23111252819170147
1201	11	-0.07943669571874068
1201	12	0.1215334953188929
1201	13	0.300302055601513
1201	14	0.23577222109757656
1201	15	0.07069540842740452
1201	16	0.23601214789837144
1201	17	0.15541905705542547
1201	18	0.07614255486608812
1201	19	0.1711993123321065
1201	20	0.0326254021486605
1201	21	0.003128637036660109
1201	22	-0.2017046803170004
1201	23	-0.0983033970100351
1201	24	-0.037176168331143344
1201	25	-0.4163600473958624
1202	1	0.05418662386832196
1202	2	-0.07114492820511131
1202	3	-0.23232960252952495
1202	4	0.01045834004104762
1202	5	-0.39997675548811173
1202	6	-0.13412591675827912
1202	7	-0.23514890557818546
1202	8	-0.020433899542922518
1202	9	0.1281744025980558
1202	10	0.10126830396426811
1202	11	0.016444659485429725
1202	12	0.12577963968779926
1202	13	0.018514379582434515
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2209	25	0.6370493381369329
2210	1	0.3622672256494468
2210	2	0.40380521535058733
2210	3	0.48892726095776595
2210	4	0.43554023895205773
2210	5	0.2665455839205819
2210	6	0.4035239816226266
2210	7	0.49419754610440236
2210	8	0.37213714986147295
2210	9	0.5110746179996823
2210	10	0.526017070147752
2210	11	0.47676782546268726
2210	12	0.41125078649040603
2210	13	0.43420048322755633
2210	14	0.4893553868763618
2210	15	0.5019858423059702
2210	16	0.5147024128343674
2210	17	0.5953442317865196
2210	18	0.5055413078392874
2210	19	0.500709625974082
2210	20	0.24061603800786457
2210	21	0.3488898349731073
2210	22	0.359227670355061
2210	23	0.548059349963232
2210	24	0.32218829277774574
2210	25	0.5229606497164667
2211	1	0.08524147932796211
2211	2	0.08578371869237245
2211	3	0.004189455046610391
2211	4	-0.10397745173155926
2211	5	0.005119730216378571
2211	6	0.13989631803998748
2211	7	0.05534883117689304
2211	8	-0.011600557796043631
2211	9	0.11556736231663223
2211	10	0.19753968434439884
2211	11	0.21754532893830714
2211	12	-0.02578913311181452
2211	13	0.029971863257934217
2211	14	-0.05684432486138746
2211	15	0.07955941068470196
2211	16	0.13971624184054576
2211	17	-0.15621486777691018
2211	18	0.09331159238027453
2211	19	0.07448998499442183
2211	20	0.23745644856881398
2211	21	0.1991876518082094
2211	22	0.2546695525543683
2211	23	0.37051812018356145
2211	24	0.1835872234377547
2211	25	0.3541500680317142
2212	1	0.4854077439312441
2212	2	0.36626225497583675
2212	3	0.38864143603570156
2212	4	0.4426677813549489
2212	5	0.3860413447618143
2212	6	0.5072014349029814
2212	7	0.4065199602326217
2212	8	0.5751150327079131
2212	9	0.427061376937921
2212	10	0.30684947308103716
2212	11	0.39544257417933437
2212	12	0.3150172869657766
2212	13	0.32096138020854426
2212	14	0.40702289278647896
2212	15	0.37769418938813004
2212	16	-0.4581020313890889
2212	17	0.08154719008199862
2212	18	0.3854933338513149
2212	19	0.35518903136181024
2212	20	0.3668378001780255
2212	21	0.3892232421357562
2212	22	0.43043152514937333
2212	23	0.43071443296094714
2212	24	0.24531314116397596
2212	25	0.4937197205519226
2213	1	0.02179920406859992
2213	2	0.1632497470962022
2213	3	-0.03226364342416943
2213	4	0.08990908058191849
2213	5	0.08998696700394504
2213	6	0.07786514878108619
2213	7	0.18839099968054995
2213	8	0.10979785676816078
2213	9	-0.024993133529548572
2213	10	-0.11342108861233413
2213	11	-0.23865218047379244
2213	12	-0.038338606999253955
2213	13	0.08350658441543857
2213	14	-0.11834952352353412
2213	15	-0.020670994248774832
2213	16	-0.11991235619583307
2213	17	0.05418827697360484
2213	18	0.10920853905353312
2213	19	-0.12091909378333554
2213	20	-0.18443372365993582
2213	21	0.04555100166596304
2213	22	0.10771081249882997
2213	23	0.14713890461085555
2213	24	-0.003923778646253595
2213	25	0.2018705781877017
2214	1	-0.09364373569723483
2214	2	-0.19815524655593464
2214	3	-0.033039493332477576
2214	4	-0.10522892665935046
2214	5	0.014194659673336218
2214	6	-0.04079874363439018
2214	7	-0.13965832556513647
2214	8	0.19024526354861848
2214	9	-0.2665116833955352
2214	10	-0.25238638696189497
2214	11	-0.28681365129713754
2214	12	-0.20540495392171465
2214	13	-0.39399964589443215
2214	14	-0.16893258648492804
2214	15	-0.23112028187749445
2214	16	-0.34900555222724705
2214	17	-0.399337137988546
2214	18	-0.3270777260520319
2214	19	0.11741376933792935
2214	20	-0.08236831716160964
2214	21	-0.0553388746682657
2214	22	0.0609423449199511
2214	23	0.025483287927734466
2214	24	0.05006832179104492
2214	25	-0.17061378726111087
2215	1	0.04076816606097822
2215	2	0.04400742141395497
2215	3	0.22325783847032454
2215	4	0.059134369433635925
2215	5	0.18719370119841017
2215	6	0.17622574104326816
2215	7	0.09137012181913917
2215	8	0.16192668618302264
2215	9	0.21803338197396727
2215	10	0.21536263656810206
2215	11	0.23014718761467634
2215	12	0.10963401016767449
2215	13	0.08060645121840082
2215	14	0.11941493455037744
2215	15	0.1366215436194551
2215	16	0.06471655065917048
2215	17	0.3564487546243811
2215	18	0.10638971800220531
2215	19	0.24025009743185421
2215	20	0.25189886624806235
2215	21	0.3005110425543549
2215	22	0.3064028485549599
2215	23	0.19912446976898934
2215	24	0.18043795972998566
2215	25	0.18057066801826238
2216	1	-0.13864368904195246
2216	2	0.1659251622687563
2216	3	-0.03422951407852892
2216	4	-0.07239604899260854
2216	5	0.045923362314340466
2216	6	-0.05596855331194206
2216	7	0.13787155714348387
2216	8	0.1327124571089442
2216	9	-0.15015589642978
2216	10	-0.10562690738601788
2216	11	-0.05428123365148707
2216	12	-0.15048733159933647
2216	13	-0.18212183296967766
2216	14	0.0666902770919151
2216	15	-0.1690668895718801
2216	16	-0.1042712229591416
2216	17	-0.03487291170419837
2216	18	-0.06252920922347016
2216	19	0.07131088510834616
2216	20	-0.009777051652612556
2216	21	0.19603356823344598
2216	22	0.3414923796003819
2216	23	0.28572769940244314
2216	24	0.1902307443821556
2216	25	0.34950988034177044
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	652.0
3	105.0
4	56.0
5	59.0
6	49.0
7	208.0
8	154.0
9	316.0
10	521.0
11	774.0
12	1379.0
13	2307.0
14	4499.0
15	7645.0
16	10739.0
17	12319.0
18	12193.0
19	11033.0
20	9924.0
21	8454.0
22	7137.0
23	6040.0
24	5354.0
25	4706.0
26	4140.0
27	3657.0
28	3236.0
29	3148.0
30	3632.0
31	4443.0
32	5407.0
33	7276.0
34	10251.0
35	16220.0
36	29216.0
37	69252.0
38	440837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.91957785372213	21.786005658592593	10.415232105513947	21.87918438217133
2	11.214077010561441	29.7361835200741	31.051512692282092	27.99822677708237
3	21.809976798801127	37.81809580197111	23.663239839206344	16.708687560021414
4	13.317272557317425	27.790241166573193	23.704657341785776	35.187828934323605
5	9.301663434610715	46.28450497132229	30.83041409506919	13.583417498997802
6	28.452232429240443	35.04947186115308	16.810661621532827	19.68763408807365
7	17.99234404496705	42.30361257412015	20.260523397680554	19.443519983232243
8	26.9428115124537	38.0237181011927	19.491827788915693	15.541642597437901
9	25.967879664154005	24.285109402016833	16.77629762220016	32.970713311629005
10	17.897618636218144	38.156914509408466	25.68839173674982	18.257075117623565
11	23.19463341908574	34.716415332360185	14.610001175143033	27.478950073411045
12	23.56334452403641	40.109254489614734	22.184353499726015	14.143047486622839
13	28.624686437232917	27.47256015943365	19.23926237855861	24.66349102477482
14	21.30937444240208	35.83453467657197	21.21945071471662	21.63664016630933
15	35.37999232467475	24.525354771375852	19.469269668728998	20.625383235220397
16	15.381434656516221	32.887410503897044	31.859479789740803	19.87167504984593
17	26.50882546605399	34.88073407486601	22.536551519056353	16.073888940023647
18	25.69703766955975	26.403810996548977	28.191490114944486	19.707661218946793
19	22.84571493295955	35.71549929915474	28.930609239830662	12.50817652805505
20	24.82478116232862	27.870593683483047	25.443658770430392	21.860966383757933
21	19.16617806593419	31.32978708337936	30.3350243167379	19.169010533948548
22	23.77411384922517	30.709557756429767	18.12526104900996	27.391067345335095
23	17.147380524857965	31.028842831491477	27.46002390352492	24.363752740125637
24	21.584550477061804	38.86952568570995	21.330193048224526	18.215730789003715
25	21.869099570438056	26.39579174427191	20.194249077629912	31.540859607660117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11927.0
1	11927.0
2	11200.0
3	10473.0
4	10473.0
5	10473.0
6	10846.5
7	11220.0
8	11220.0
9	11220.0
10	10714.0
11	10208.0
12	10208.0
13	10208.0
14	9952.0
15	9696.0
16	9696.0
17	9696.0
18	10688.0
19	11680.0
20	11680.0
21	11680.0
22	13415.0
23	15150.0
24	15150.0
25	15150.0
26	17628.5
27	20107.0
28	20107.0
29	20107.0
30	21231.0
31	22355.0
32	22355.0
33	22355.0
34	22333.5
35	22312.0
36	22312.0
37	22312.0
38	23142.0
39	23972.0
40	23972.0
41	23972.0
42	33857.5
43	43743.0
44	43743.0
45	43743.0
46	143851.5
47	243960.0
48	243960.0
49	243960.0
50	201243.0
51	158526.0
52	158526.0
53	158526.0
54	106439.5
55	54353.0
56	54353.0
57	54353.0
58	36714.0
59	19075.0
60	19075.0
61	19075.0
62	14889.5
63	10704.0
64	10704.0
65	10704.0
66	7758.5
67	4813.0
68	4813.0
69	4813.0
70	3261.5
71	1710.0
72	1710.0
73	1710.0
74	1296.0
75	882.0
76	882.0
77	882.0
78	610.5
79	339.0
80	339.0
81	339.0
82	216.0
83	93.0
84	93.0
85	93.0
86	63.0
87	33.0
88	33.0
89	33.0
90	18.0
91	3.0
92	3.0
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	2.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013430637121150002
2	0.18081878818895636
3	0.18944267097201054
4	0.17954641204063687
5	0.1963700522239721
6	0.16668127542985106
7	0.17261903078867527
8	0.14731288294987688
9	0.16696402568503318
10	0.17982916229581897
11	0.1471715078222858
12	0.15508851496738477
13	0.18930129584441951
14	0.16738815106780633
15	0.16668127542985106
16	0.16427789826080316
17	0.1615917708365732
18	0.16555027440912265
19	0.14816113371542317
20	0.17261903078867527
21	0.1753051582129053
22	0.14886800935337846
23	0.16484339877116738
24	0.17601203385086056
25	0.6402879528598774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	707338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.141775865686583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94434565128489	16.10221091837156
2	10.208833616286533	4.1124807709866
3	3.5798648346272963	2.1631450538554846
4	1.699365369392554	1.3691294553685802
5	0.9594635201461542	0.9662649587043252
6	0.615056599586646	0.7432999304151339
7	0.39944695644878025	0.5631899746915401
8	0.3199728178888848	0.5155856624824054
9	0.2357311999870297	0.4273240495219283
>10	1.5924552742594436	6.336538298105763
>50	0.20273880671896768	2.8546486700297113
>100	0.1753795905873423	7.225851523865239
>500	0.02665769776927603	3.8016295830891154
>1k	0.02385162431987855	11.176409468990256
>5k	0.009119738710541798	11.47045114014463
>10k+	0.00771670198584306	30.171840541377737
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	44929	6.3518431075384045	No Hit
CCCATGTACTCTGCGTTGATACCAC	41219	5.827341384175599	No Hit
GAGTACATGGGAAGCAGTGGTATCA	22239	3.1440414624974196	No Hit
CATGTACTCTGCGTTGATACCACTG	17871	2.526514905179702	No Hit
GCGTTGATACCACTGCTTCCCATGT	14661	2.072700745612423	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	13942	1.9710520288744562	No Hit
TATCAACGCAGAGTACATGGGAAGC	12262	1.7335418145214876	No Hit
GTATCAACGCAGAGTACATGGGAAG	12126	1.7143147971691044	No Hit
GGTATCAACGCAGAGTACATGGGAA	12062	1.705266789003277	No Hit
ACTCTGCGTTGATACCACTGCTTCC	11756	1.6620059999604149	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10466	1.479632085367957	No Hit
GTGGTATCAACGCAGAGTACATGGG	8462	1.1963163296754875	No Hit
GTACTCTGCGTTGATACCACTGCTT	7770	1.0984847413824792	No Hit
GCTTCCCATGTACTCTGCGTTGATA	7524	1.06370645999508	No Hit
ATACCACTGCTTCCCATGTACTCTG	6546	0.9254415852110306	No Hit
GATACCACTGCTTCCCATGTACTCT	6279	0.8876944261442196	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6148	0.8691742844297917	No Hit
CAGTGGTATCAACGCAGAGTACATG	6067	0.8577228990949164	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	5825	0.8235101182178817	No Hit
CATGGGAAGCAGTGGTATCAACGCA	5520	0.7803907043026107	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	5453	0.77091857075401	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	5378	0.7603154361846811	No Hit
GCAGTGGTATCAACGCAGAGTACAT	5117	0.7234165278834164	No Hit
GTTGATACCACTGCTTCCCATGTAC	5090	0.7195993994384581	No Hit
TACCACTGCTTCCCATGTACTCTGC	4831	0.6829832413923754	No Hit
ACATGGGAAGCAGTGGTATCAACGC	4682	0.6619183473813085	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4551	0.6433982056668807	No Hit
CCACTGCTTCCCATGTACTCTGCGT	4411	0.6236056878041333	No Hit
CTTCCCATGTACTCTGCGTTGATAC	4211	0.5953306622859227	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4051	0.5727106418713543	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	3916	0.5536249996465622	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3777	0.5339738569114059	No Hit
CCATGTACTCTGCGTTGATACCACT	3662	0.5177157172384348	No Hit
TGATACCACTGCTTCCCATGTACTC	3305	0.467244796688429	No Hit
TTCCCATGTACTCTGCGTTGATACC	3193	0.4514107823982311	No Hit
ATCAACGCAGAGTACATGGGAAGCA	2701	0.3818542196234332	No Hit
GTACATGGTAAGCAGTGGTATCAAC	2357	0.33322117573211113	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2109	0.29816014408953	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	1910	0.2700264936989106	No Hit
ACCATGTACTCTGCGTTGATACCAC	1816	0.2567372317053516	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1759	0.24867884943266164	No Hit
CTGCGTTGATACCACTGCTTCCCAT	1613	0.2280380808043679	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	1592	0.22506920312495585	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1535	0.21701082085226583	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1525	0.21559706957635527	No Hit
CTCTGCGTTGATACCACTGCTTCCC	1522	0.21517294419358216	No Hit
TCAACGCAGAGTACATGGGAAGCAG	1392	0.19679417760674528	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	1322	0.1868979186753716	No Hit
TCCATGTACTCTGCGTTGATACCAC	1298	0.18350491561318633	No Hit
AAAGTACTCTGCGTTGATACCACTG	1245	0.17601203385086056	No Hit
CTGTACTTGTTCGCTATCGGTCTCT	1190	0.16823640183335264	No Hit
AGTGGTATCAACGCAGAGTACATGG	1151	0.1627227718573016	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	1115	0.1576332672640237	No Hit
TCCCATGTACTCTGCGTTGATACCA	1095	0.15480576471220267	No Hit
TCACTGTACTTGTTCGCTATCGGTC	1074	0.15183688703279055	No Hit
CCCCATGTACTCTGCGTTGATACCA	1069	0.15113001139483528	No Hit
AACGCAGAGTACATGGGAAGCAGTG	1065	0.15056451088447106	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1053	0.14886800935337846	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	981	0.13868900016682265	No Hit
GTACTAGGGGTTGCCCCATTCGGAT	952	0.13458912146668214	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	936	0.13232711942522526	No Hit
GTACATGGGTAAGCAGTGGTATCAA	921	0.1302064925113595	No Hit
GTACTTGTTCGCTATCGGTCTCTCG	920	0.13006511738376844	No Hit
GAGTACATGGTAAGCAGTGGTATCA	920	0.13006511738376844	No Hit
GTACATGGAAAGCAGTGGTATCAAC	899	0.12709623970435635	No Hit
ATGTACTCTGCGTTGATACCACTGC	898	0.12695486457676528	No Hit
TTGATACCACTGCTTCCCATGTACT	894	0.12638936406640106	No Hit
CAACGCAGAGTACATGGGAAGCAGT	821	0.11606897975225422	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	807	0.11408972796597949	No Hit
GTGGTATCAACGCAGAGTACTTTTT	797	0.11267597669006896	No Hit
AGCTAATACGGCTGATGGTGGATAC	788	0.11140360054174948	No Hit
GCTTACCATGTACTCTGCGTTGATA	787	0.11126222541415842	No Hit
GAGTACATGGAAAGCAGTGGTATCA	762	0.10772784722438211	No Hit
TTCCATCACTGTACTTGTTCGCTAT	726	0.10263834263110423	No Hit
GTACTGGTTCACTATCGGTCACACA	720	0.1017900918655579	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	716	0.1012245913551937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4137512759105264E-4	0.0
2	0.0	0.0	0.0	1.4137512759105264E-4	0.0
3	0.0	0.0	0.0	1.4137512759105264E-4	0.0
4	0.0	0.0	0.0	1.4137512759105264E-4	0.0
5	0.0	0.0	0.0	1.4137512759105264E-4	0.0
6	0.0	0.0	0.0	1.4137512759105264E-4	0.0
7	0.0	0.0	0.0	1.4137512759105264E-4	0.0
8	0.0	0.0	0.0	1.4137512759105264E-4	0.0
9	0.0	0.0	0.0	1.4137512759105264E-4	0.0
10	0.0	0.0	0.0	1.4137512759105264E-4	0.0
11	0.0	0.0	0.0	1.4137512759105264E-4	0.0
12	0.0	0.0	0.0	1.4137512759105264E-4	0.0
13	0.0	0.0	0.0	1.4137512759105264E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	120	0.0	18.208063	1
GTCCCGC	40	2.7662178E-4	16.618864	12
GCACAAA	35	0.0021688372	16.285471	1
TAACAAA	60	1.4642701E-6	15.833097	1
GACCAAA	55	1.1267266E-5	15.545222	1
GAACAAA	715	0.0	14.880897	1
CCGCCCT	45	6.7747984E-4	14.772323	15
TGTCCCG	45	6.7747984E-4	14.772323	11
GCTAAAA	65	3.3663455E-6	14.615167	1
CGCAAAA	85	1.711669E-8	14.529195	2
CTAGTGC	40	0.005136045	14.307572	19
GTAAAAA	60	2.5647032E-5	14.249788	1
GTCCCTG	40	0.0052882624	14.2447405	11
TCCTGAA	70	7.2502316E-6	13.571226	2
GGAAACA	85	2.69245E-7	13.411565	1
CCCTACT	50	0.0015015044	13.296033	18
TCCCGCC	50	0.0015023062	13.295093	13
CCCGCCC	50	0.0015023062	13.295093	14
TACAAAA	250	0.0	12.919807	2
ATCCTGA	85	3.935358E-6	12.293935	1
>>END_MODULE