FastQCFastQC Report
Wed 25 May 2016
SRR1294847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707338
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC452746.400617526557317No Hit
CCCATGTACTCTGCGTTGATACCAC431236.096519627108964No Hit
GAGTACATGGGAAGCAGTGGTATCA233303.2982817266992583No Hit
CATGTACTCTGCGTTGATACCACTG183122.588861336447356No Hit
GTATCAACGCAGAGTACTTTTTTTT182612.5816512049402123No Hit
ACGCAGAGTACATGGGAAGCAGTGG153062.163887702908652No Hit
GCGTTGATACCACTGCTTCCCATGT149892.119071787462288No Hit
GGTATCAACGCAGAGTACTTTTTTT139221.9682245263226352No Hit
TATCAACGCAGAGTACATGGGAAGC130101.839290409959595No Hit
GTATCAACGCAGAGTACATGGGAAG125711.7772267289471229No Hit
GGTATCAACGCAGAGTACATGGGAA125121.7688855964192507No Hit
ACTCTGCGTTGATACCACTGCTTCC122851.7367934424560818No Hit
TATCAACGCAGAGTACTTTTTTTTT120051.697208406730587No Hit
GTGGTATCAACGCAGAGTACATGGG85791.2128572196036407No Hit
GCTTCCCATGTACTCTGCGTTGATA77861.100746743423936No Hit
GTACTCTGCGTTGATACCACTGCTT73471.0386830624114638No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68550.9691264996366659No Hit
CAGTGGTATCAACGCAGAGTACATG68190.964036995043388No Hit
GGGAAGCAGTGGTATCAACGCAGAG66590.9414169746288196No Hit
GTACTTTTTTTTTTTTTTTTTTTTT66040.9336413426113117No Hit
GATACCACTGCTTCCCATGTACTCT65850.9309552151870817No Hit
ATACCACTGCTTCCCATGTACTCTG65400.9245933344454843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA63380.8960355586720916No Hit
CATGGGAAGCAGTGGTATCAACGCA61810.8738396636402964No Hit
GCAGAGTACATGGGAAGCAGTGGTA57630.8147448603072365No Hit
ACATGGGAAGCAGTGGTATCAACGC54760.7741701986886044No Hit
GCAGTGGTATCAACGCAGAGTACAT54550.7712013210091923No Hit
CTGCTTCCCATGTACTCTGCGTTGA54100.7648394402675949No Hit
GTTGATACCACTGCTTCCCATGTAC51690.7307680345181511No Hit
TACCACTGCTTCCCATGTACTCTGC49020.6930208754513401No Hit
CCACTGCTTCCCATGTACTCTGCGT46010.6504669620464332No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44820.6336433218630979No Hit
CTTCCCATGTACTCTGCGTTGATAC44750.6326536959699606No Hit
AAGCAGTGGTATCAACGCAGAGTAC43110.6094681750450279No Hit
ATGGGAAGCAGTGGTATCAACGCAG42060.5946237866479674No Hit
CCATGTACTCTGCGTTGATACCACT39150.5534836245189712No Hit
TGATACCACTGCTTCCCATGTACTC33720.47671693023702955No Hit
TTCCCATGTACTCTGCGTTGATACC31780.44929015548436535No Hit
GCAGAGTACTTTTTTTTTTTTTTTT29100.41140162128996316No Hit
ATCAACGCAGAGTACATGGGAAGCA28160.3981123592964043No Hit
GTACATGGTAAGCAGTGGTATCAAC24570.34735868849121637No Hit
GTGGTATCAACGCAGAGTACTTTTT24120.340996807749619No Hit
GGAAGCAGTGGTATCAACGCAGAGT21750.3074909025105395No Hit
ACCATGTACTCTGCGTTGATACCAC18870.26677486576431636No Hit
ATCAACGCAGAGTACTTTTTTTTTT18630.2633818627021311No Hit
AAAAAGTACTCTGCGTTGATACCAC16170.22860358131473213No Hit
CTGCGTTGATACCACTGCTTCCCAT15750.22266582595590795No Hit
CTCTGCGTTGATACCACTGCTTCCC15600.22054519904204212No Hit
TCAACGCAGAGTACATGGGAAGCAG14830.2096593142175311No Hit
TGGGAAGCAGTGGTATCAACGCAGA14540.20555943551739056No Hit
AGTGGTATCAACGCAGAGTACATGG12920.18265666484764004No Hit
GGTAAGCAGTGGTATCAACGCAGAG12760.1803946628061832No Hit
TCCATGTACTCTGCGTTGATACCAC12120.1713466546403558No Hit
AAAGTACTCTGCGTTGATACCACTG12060.1704984038748095No Hit
CTGTACTTGTTCGCTATCGGTCTCT11760.16625715004707792No Hit
AACGCAGAGTACATGGGAAGCAGTG11740.16597439979189582No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA11410.16130902058139107No Hit
CCCCATGTACTCTGCGTTGATACCA11310.15989526930548054No Hit
TCACTGTACTTGTTCGCTATCGGTC10950.15480576471220267No Hit
TCCCATGTACTCTGCGTTGATACCA10730.1516955119051995No Hit
ACTGCTTCCCATGTACTCTGCGTTG10700.15127138652242633No Hit
GTACATGGAAAGCAGTGGTATCAAC10310.1457577565463753No Hit
GAGTACATGGTAAGCAGTGGTATCA9890.13982000118755109No Hit
GTACATGGGTAAGCAGTGGTATCAA9640.13628562299777475No Hit
TTGATACCACTGCTTCCCATGTACT9220.13034786763895054No Hit
CAACGCAGAGTACATGGGAAGCAGT9160.12949961687340422No Hit
CAACGCAGAGTACTTTTTTTTTTTT8910.1259652386836279No Hit
GTACTAGGGGTTGCCCCATTCGGAT8820.12469286253530844No Hit
ATGTACTCTGCGTTGATACCACTGC8340.11790685641093791No Hit
GTACTTGTTCGCTATCGGTCTCTCG8280.1170586056453916No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT8250.11663448026261844No Hit
AGCTAATACGGCTGATGGTGGATAC8130.1149379787315258No Hit
CGTTGATACCACTGCTTCCCATGTA8080.11423110309357054No Hit
GAGTACATGGAAAGCAGTGGTATCA8050.1138069777107974No Hit
GCTTACCATGTACTCTGCGTTGATA7730.1092829736278837No Hit
TTCCATCACTGTACTTGTTCGCTAT7550.10673822133124475No Hit
CGCAGAGTACATGGGAAGCAGTGGT7530.10645547107606265No Hit
GTACTGGTTCACTATCGGTCACACA7470.10560722031051632No Hit
AACGCAGAGTACTTTTTTTTTTTTT7440.10518309492774318No Hit
GGTATCAACGCAGAGTACATGGGAT7410.10475896954497002No Hit
ACTCTGCGTTGATACCACTGCTTAC7380.10433484416219685No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT7290.10306246801387738No Hit
ACGCAGAGTACATGGTAAGCAGTGG7140.10094184110001159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC250.00591037519.065411
CGTATCA250.005920082319.0599962
TAAGGCA250.005920082319.0599965
CGATAGT851.7378625E-814.51243718
GAACAAA2450.014.3963321
GTACAGG400.00515638314.2990581
GCCATGT500.00145982413.3457871
GATAGTG1155.344191E-912.38470719
ATACTAC550.002994038612.1290893
GCGCCAG1103.866444E-812.07592912
CGCCAGG1103.866444E-812.07592913
ACAGAAA802.7703045E-511.9124983
AGGTTTC1302.6502676E-911.67864717
TCCATCA1406.4210326E-1011.5721412
AAAAGTA3400.011.2117632
CACAGAA855.1451243E-511.2117632
GCCAGGT1451.2350938E-911.12413214
AACAACT600.005924710511.0696017
CAGAAAA951.31055E-511.0347364
CAGGTTT1408.463758E-910.84445916
Produced by FastQC (version 0.11.5)