FastQCFastQC Report
Wed 25 May 2016
SRR1294846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741175
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC317674.286032313556178No Hit
CCCATGTACTCTGCGTTGATACCAC299724.043849293351773No Hit
GAGTACATGGGAAGCAGTGGTATCA161812.1831551253077883No Hit
CATGTACTCTGCGTTGATACCACTG131061.76827334974871No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA128851.7384558302695043No Hit
GCGTTGATACCACTGCTTCCCATGT107261.4471616015111142No Hit
ACGCAGAGTACATGGGAAGCAGTGG103081.3907646642156035No Hit
GGTATCAACGCAGAGTACATGGGAA92681.2504469254899315No Hit
GTATCAACGCAGAGTACATGGGAAG88991.2006611124228421No Hit
TATCAACGCAGAGTACATGGGAAGC88361.1921610955577293No Hit
ACTCTGCGTTGATACCACTGCTTCC86251.1636927851047323No Hit
GTGGTATCAACGCAGAGTACATGGG62510.8433905622828617No Hit
GTACTCTGCGTTGATACCACTGCTT58920.7949539582419807No Hit
GCTTCCCATGTACTCTGCGTTGATA55860.7536681620400041No Hit
ATACCACTGCTTCCCATGTACTCTG48350.6523425641717543No Hit
GATACCACTGCTTCCCATGTACTCT47110.6356123722467704No Hit
CAGTGGTATCAACGCAGAGTACATG46600.6287314062131076No Hit
GGGAAGCAGTGGTATCAACGCAGAG43750.5902789489661686No Hit
CTGCTTCCCATGTACTCTGCGTTGA43250.5835329038351267No Hit
CATGGGAAGCAGTGGTATCAACGCA41440.5591122204607549No Hit
GCAGAGTACATGGGAAGCAGTGGTA40700.5491280736668128No Hit
GTTGATACCACTGCTTCCCATGTAC39260.5296994636894121No Hit
GCAGTGGTATCAACGCAGAGTACAT38800.5234931021688536No Hit
TACCACTGCTTCCCATGTACTCTGC36240.4889533510979188No Hit
GTATCAACGCAGAGTACTTTTTTTT34400.46412790501568457No Hit
CCACTGCTTCCCATGTACTCTGCGT34360.46358822140520123No Hit
ACATGGGAAGCAGTGGTATCAACGC33380.450365972948359No Hit
CTTCCCATGTACTCTGCGTTGATAC32670.44078658886227945No Hit
AAGCAGTGGTATCAACGCAGAGTAC29650.4000404762707862No Hit
CCATGTACTCTGCGTTGATACCACT28880.38965156676898166No Hit
ATGGGAAGCAGTGGTATCAACGCAG28700.3872229905218066No Hit
GGTATCAACGCAGAGTACTTTTTTT28370.38277060073531893No Hit
TGATACCACTGCTTCCCATGTACTC24930.33635781023375044No Hit
TATCAACGCAGAGTACTTTTTTTTT22230.29992916652612406No Hit
TTCCCATGTACTCTGCGTTGATACC21110.28481802543259016No Hit
GTACATGGTAAGCAGTGGTATCAAC20930.2823894491854151No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA19830.2675481498971228No Hit
ATCAACGCAGAGTACATGGGAAGCA19500.2630957601106351No Hit
ACCATGTACTCTGCGTTGATACCAC16320.22019091307720848No Hit
AAACAAAAAAAAAAAAAAAAAAAAA14750.19900833136573684No Hit
GGAAGCAGTGGTATCAACGCAGAGT14410.1944210206766283No Hit
CTGCGTTGATACCACTGCTTCCCAT12960.17485748979660673No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12640.17054002091273993No Hit
AAAAAGTACTCTGCGTTGATACCAC11720.15812729787162275No Hit
CTCTGCGTTGATACCACTGCTTCCC10760.14517489122002225No Hit
TCAACGCAGAGTACATGGGAAGCAG10590.142881235875468No Hit
GGTAAGCAGTGGTATCAACGCAGAG10370.13991297601780955No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10130.13667487435490944No Hit
TGGGAAGCAGTGGTATCAACGCAGA10030.13532566532870105No Hit
CCCCATGTACTCTGCGTTGATACCA9720.13114311734745504No Hit
GTACATGGGTAAGCAGTGGTATCAA8850.1194049988194421No Hit
AAAGTACTCTGCGTTGATACCACTG8790.11859547340371708No Hit
AGTGGTATCAACGCAGAGTACATGG8750.11805578979323372No Hit
GAACAAAAAAAAAAAAAAAAAAAAA8480.11441292542247107No Hit
TCCCATGTACTCTGCGTTGATACCA8280.1117145073700543No Hit
GAGTACATGGTAAGCAGTGGTATCA8140.10982561473336257No Hit
TCCATGTACTCTGCGTTGATACCAC8000.10793672209667082No Hit
ACTGCTTCCCATGTACTCTGCGTTG7980.10766688029142915No Hit
GTACATGGAAAGCAGTGGTATCAAC7680.103619253212804No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7640.10307956960232065No Hit
AACGCAGAGTACATGGGAAGCAGTG7630.10294464869969981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC755.8216756E-815.2020031
GAACAAA5750.015.2020021
ACACCAT456.7878247E-414.7687386
GTGTAGC551.9557796E-413.8200021
GTACAAA1351.8189894E-1213.3721311
CTCCAAA604.0829807E-412.6683351
GTACATA600.00586497111.0847931
TCCCACA600.00586497111.0847932
ACCGTGC600.005882710711.0802978
TACAAAA2100.010.8585732
CCCTTAG803.7610583E-410.6889081
GAACATA803.7610583E-410.6889081
AGGACTG1252.2325366E-710.6399625
ACTGCAT1002.4009605E-510.4471388
TTAGGAC1106.020735E-610.3650023
CCTTAGG1106.020735E-610.3650021
GCTCTGA750.002641877410.1346681
CCTGGAC750.002641877410.1346683
TAGGACT1054.0934225E-59.9530194
GACTGCA1054.1117135E-59.9489827
Produced by FastQC (version 0.11.5)