FastQCFastQC Report
Wed 25 May 2016
SRR1294846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741175
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC322374.3494451377879715No Hit
CCCATGTACTCTGCGTTGATACCAC316884.275373562249132No Hit
GAGTACATGGGAAGCAGTGGTATCA167422.258845751678079No Hit
CATGTACTCTGCGTTGATACCACTG137131.8501703376395588No Hit
GTATCAACGCAGAGTACTTTTTTTT115631.560090397004756No Hit
GCGTTGATACCACTGCTTCCCATGT114151.5401221034168717No Hit
ACGCAGAGTACATGGGAAGCAGTGG109961.4835902452187406No Hit
TATCAACGCAGAGTACATGGGAAGC94881.280129524066516No Hit
GGTATCAACGCAGAGTACTTTTTTT94801.2790501568455495No Hit
GGTATCAACGCAGAGTACATGGGAA93691.2640739366546363No Hit
GTATCAACGCAGAGTACATGGGAAG92081.2423516713326812No Hit
ACTCTGCGTTGATACCACTGCTTCC90691.2235976658683847No Hit
TATCAACGCAGAGTACTTTTTTTTT78791.063041791749587No Hit
GTGGTATCAACGCAGAGTACATGGG64470.869835059196546No Hit
GCTTCCCATGTACTCTGCGTTGATA59290.7999460316389517No Hit
GTACTCTGCGTTGATACCACTGCTT56450.7616284952946336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA54600.7366681283097783No Hit
GATACCACTGCTTCCCATGTACTCT50040.6751441967146761No Hit
CAGTGGTATCAACGCAGAGTACATG50040.6751441967146761No Hit
ATACCACTGCTTCCCATGTACTCTG48800.658414004789692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48370.652612405976996No Hit
GGGAAGCAGTGGTATCAACGCAGAG47730.6439774682092624No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45910.6194218639322697No Hit
CATGGGAAGCAGTGGTATCAACGCA45350.6118662933855028No Hit
CTGCTTCCCATGTACTCTGCGTTGA43760.5904138698687894No Hit
GCAGAGTACATGGGAAGCAGTGGTA41910.5654535028839343No Hit
GCAGTGGTATCAACGCAGAGTACAT41410.5587074577528924No Hit
GTTGATACCACTGCTTCCCATGTAC39150.5282153337605828No Hit
ACATGGGAAGCAGTGGTATCAACGC37410.504739096704557No Hit
TACCACTGCTTCCCATGTACTCTGC37130.5009613114311735No Hit
CCACTGCTTCCCATGTACTCTGCGT35920.484635882214052No Hit
GAGTACTTTTTTTTTTTTTTTTTTT34340.46331837959995953No Hit
CTTCCCATGTACTCTGCGTTGATAC33390.4505008938509799No Hit
AAGCAGTGGTATCAACGCAGAGTAC33130.4469929503828381No Hit
ATGGGAAGCAGTGGTATCAACGCAG30880.4166357472931494No Hit
CCATGTACTCTGCGTTGATACCACT30020.40503254966775726No Hit
TGATACCACTGCTTCCCATGTACTC25530.34445306439100076No Hit
TTCCCATGTACTCTGCGTTGATACC21690.2926434377845988No Hit
GTACATGGTAAGCAGTGGTATCAAC21430.289135494316457No Hit
ATCAACGCAGAGTACATGGGAAGCA20660.2787465848146524No Hit
GCAGAGTACTTTTTTTTTTTTTTTT20470.2761830876648565No Hit
GTGGTATCAACGCAGAGTACTTTTT17080.23044490167639223No Hit
ACCATGTACTCTGCGTTGATACCAC16780.22639727459776707No Hit
GGAAGCAGTGGTATCAACGCAGAGT15940.21506391877761663No Hit
ATCAACGCAGAGTACTTTTTTTTTT12720.1716193881337066No Hit
CTGCGTTGATACCACTGCTTCCCAT12510.16878604917866902No Hit
AAAAAGTACTCTGCGTTGATACCAC12100.16325429217121462No Hit
TCAACGCAGAGTACATGGGAAGCAG11590.15637332613755187No Hit
CTCTGCGTTGATACCACTGCTTCCC11230.1515161736432017No Hit
GGTAAGCAGTGGTATCAACGCAGAG11200.15111141093533917No Hit
TGGGAAGCAGTGGTATCAACGCAGA10930.1474685465645765No Hit
CCCCATGTACTCTGCGTTGATACCA9360.12628596485310487No Hit
AGTGGTATCAACGCAGAGTACATGG9340.1260161230478632No Hit
GTACATGGGTAAGCAGTGGTATCAA9240.12466691402165479No Hit
TCCATGTACTCTGCGTTGATACCAC8920.12034944513778797No Hit
GAGTACATGGTAAGCAGTGGTATCA8900.12007960333254629No Hit
AAAGTACTCTGCGTTGATACCACTG8610.11616689715654198No Hit
TCCCATGTACTCTGCGTTGATACCA8590.11589705535130031No Hit
AACGCAGAGTACATGGGAAGCAGTG8500.11468276722771276No Hit
ACTGCTTCCCATGTACTCTGCGTTG7670.10348433231018314No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7560.10200020238135393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGC250.00592259219.0587443
TGGCACT250.00607249718.97645618
TAATGCA350.002124483716.3360654
GAACAAA2000.014.7745311
CAGTCCT456.82024E-414.7594659
TCGCACT400.00516867714.2940584
AAGTGTG400.005316849314.2333017
TAGACTG550.002995748512.1282915
CCCTTAG1001.8486571E-611.4383471
TGGCCAG855.14935E-511.2110265
ACCAGGA600.00573444611.1183542
CCAGGAC600.0057373411.11763
CTGCGCT600.005925175711.0695989
ATCGTTC700.001497688910.85027719
ACGCAAA951.5864824E-410.0336371
CCTTAGG1002.6646466E-49.5300182
TTATACT800.0043972829.5293724
ACACGGT900.00111999.492716
GAAAAAA6700.09.1051511
CACGGTG950.00184064878.9894537
Produced by FastQC (version 0.11.5)