FastQCFastQC Report
Wed 25 May 2016
SRR1294845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518895
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC54730.21727781427967421No Hit
CCCATGTACTCTGCGTTGATACCAC47410.18821745249404997No Hit
GAGTACATGGGAAGCAGTGGTATCA25280.10036146802466954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG905.394577E-712.6695335
ACGCGCG604.112611E-412.65970616
GTCTTAG1657.2759576E-1211.5172981
GACGCGC700.001498813910.85117715
CTGTCGC1502.1445885E-910.7635339
CCTCGAT1158.860534E-710.73557811
ATTAGAC1951.4551915E-1110.23267653
TATACCG750.002643578510.1356265
AACTGCG750.002654947610.13038357
ACTTCGA750.00266064810.12776518
TAGACAG4050.09.6194595
CTTACAC4150.09.6162513
GTATTAT4300.09.5017711
GTGCTAG2401.8189894E-129.5017711
GTCGTAG1002.751201E-49.5017711
CGCGCGG900.00111963099.4947817
TCTACAC3650.09.3716113
GTCCTAG3150.09.3509491
TACACTG6150.09.27039055
GTATTAA4100.09.2700211
Produced by FastQC (version 0.11.5)