FastQCFastQC Report
Wed 25 May 2016
SRR1294845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518895
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73560.292032815976847No Hit
GTACATGGGAAGCAGTGGTATCAAC54990.21831001292233304No Hit
CCCATGTACTCTGCGTTGATACCAC49950.1983012392338704No Hit
GGTATCAACGCAGAGTACTTTTTTT44680.17737936674613275No Hit
TATCAACGCAGAGTACTTTTTTTTT40950.1625712862187586No Hit
GAGTACATGGGAAGCAGTGGTATCA25840.10258466510116539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA701.0976796E-412.20680319
AACGATT550.00309818212.07582617
CGCGCCA550.003098642712.07558510
GCACCGT802.8884022E-511.8667826
TACGTCG600.005738663511.118955
TTGCGGA803.7938444E-410.68095319
ACTTGCG750.00257897510.1658965
ACGGGTC750.00257897510.1658965
TCCTATA2950.010.0157312
TTCCGTA1053.9620732E-59.984962
GCTGCGC1304.0708564E-69.5305283
TGCACCG1106.603293E-59.5305275
GCGCTGA1002.77559E-49.4934256
GTAATAC2850.09.3633253
CTGCGCT1651.01488695E-79.2417244
TACACCG1054.3520587E-49.0766935
GTCATAG2950.09.048091
CACGGGT950.0017717819.0289214
CTAGGAC2850.09.0289213
TAGACAG3700.09.0153655
Produced by FastQC (version 0.11.5)