FastQCFastQC Report
Wed 25 May 2016
SRR1294844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880476
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC201720.5198331338732671No Hit
CCCATGTACTCTGCGTTGATACCAC175720.45283104443887806No Hit
GAGTACATGGGAAGCAGTGGTATCA93670.2413879122045852No Hit
CATGTACTCTGCGTTGATACCACTG69070.1779936275858941No Hit
GATCAGCACCATGAAGCTTCTCACG64700.1667321225540372No Hit
GCGTTGATACCACTGCTTCCCATGT64080.165134380421371No Hit
GTATCAACGCAGAGTACATGGGAAG58600.15101240157135362No Hit
GTATCAACGCAGAGTACTTTTTTTT53070.13676157254934704No Hit
ACGCAGAGTACATGGGAAGCAGTGG47390.12212419301137283No Hit
GGTATCAACGCAGAGTACATGGGAA43490.11207387959621448No Hit
TATCAACGCAGAGTACATGGGAAGC43160.1112234684610857No Hit
GTGGTATCAACGCAGAGTACATGGG40370.10403362886408782No Hit
ACTCTGCGTTGATACCACTGCTTCC39440.10163701566508851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT500.00150166713.2983378
TCTCACG11300.011.73226119
GTACTAG1701.0913936E-1111.1778161
TAAACCG700.001489811610.8592925
CATACCG803.766521E-410.6896145
AGACCGT2150.010.5988076
TCTCGTA1252.4455749E-69.91924519
AGACGTA1252.5662011E-69.8819545
TTGGCGT1002.7513923E-49.5020034
TAGACAG5500.09.501885
CGGATAG800.0045106519.501885
ACGAGCG900.00111228879.501885
CGTACAC900.0011134729.5007753
ACCGTCT1401.0615568E-69.4988138
CCGATCG800.00452268179.4988129
CGATCGC800.00452268179.49881210
CGCGGTC900.00111953799.49513318
TCGTCTA1002.774324E-49.49415314
GTCTAGA3250.09.3549721
TAGAACC2700.09.1500774
Produced by FastQC (version 0.11.5)