FastQCFastQC Report
Wed 25 May 2016
SRR1294844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880476
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC203370.524085189548911No Hit
GTATCAACGCAGAGTACTTTTTTTT187430.483007754718751No Hit
CCCATGTACTCTGCGTTGATACCAC183790.4736274621979365No Hit
GGTATCAACGCAGAGTACTTTTTTT118640.30573568809599644No Hit
TATCAACGCAGAGTACTTTTTTTTT106120.2734716050298984No Hit
GAGTACATGGGAAGCAGTGGTATCA96260.24806235111362626No Hit
CATGTACTCTGCGTTGATACCACTG70810.1824776135711186No Hit
GCGTTGATACCACTGCTTCCCATGT65590.1690256556154451No Hit
GTATCAACGCAGAGTACATGGGAAG61420.15827955127154503No Hit
GATCAGCACCATGAAGCTTCTCACG60390.15562523772856732No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56240.14493067345346294No Hit
ACGCAGAGTACATGGGAAGCAGTGG50970.13134986532580023No Hit
TATCAACGCAGAGTACATGGGAAGC46080.11874831850525554No Hit
GGTATCAACGCAGAGTACATGGGAA43710.112640820352967No Hit
ACTCTGCGTTGATACCACTGCTTCC42440.10936802598444108No Hit
GCTTCCCATGTACTCTGCGTTGATA40500.10436863931125974No Hit
GTGGTATCAACGCAGAGTACATGGG40030.10315744769456119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG1153.8562575E-1013.2012659
GGTCGGC658.1108016E-411.67804211
CGTCCGC1605.5661076E-1010.6744610
CCTATAC3800.010.5325173
GCTCGTT2100.010.39206811
CTGCGTA1201.5495134E-610.279119
TCTCACG10600.010.21017319
CGTAAAT750.002675486510.12123215
CGGTACT856.4127485E-410.0899754
GTCCCGC1803.7107384E-1010.062741
CTATACT3800.09.5294214
CCGATCA2950.09.3327619
GCATCGT2054.292815E-109.261526
CGCATCG1651.0174881E-79.2406515
GTCCAGG9450.09.1800431
TTAGCAC2600.09.1629043
ATTCCGA1356.917493E-69.1414646
GACTCCG1252.7954275E-59.1091087
CCGACCA1054.550485E-49.036589
GTATAGA4550.09.0093231
Produced by FastQC (version 0.11.5)