FastQCFastQC Report
Wed 25 May 2016
SRR1294843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656611
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC101940.21891457113338433No Hit
CCCATGTACTCTGCGTTGATACCAC92630.19892149032848136No Hit
ATTCCATTCCATTCCATTCCATTCC68670.14746776142563767No Hit
GTATCAACGCAGAGTACTTTTTTTT58730.12612176537829767No Hit
GAATGGAATGGAATGGAATGGAATG56370.12105370193043825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA905.454149E-712.65870514
AGGCCGT700.00149793110.85230356
GACCGTC1002.4064539E-510.4479277
TGGATCG1201.5209298E-610.2941695
AGACCGT1303.8706276E-710.2262096
CCATCCG750.002653628810.1313259
TTTCGTC1451.6337253E-79.82172617
GTATAGG5450.09.2404791
GTATACG1151.1090046E-49.0888731
GGCGTAT850.00746863438.93690318
TAGACAG6400.08.9084165
CGAATTA1951.6694685E-88.76371915
AGTACCG1201.7605304E-48.7104515
CGCTAAC1201.7772544E-48.7028615
TCTATAC6600.08.6378133
TAGGACA6100.08.5677494
GTCCTAT3450.08.5380321
TCAGTAC5800.08.518673
GTCTTAT5700.08.5017931
ACACCGT1356.839364E-58.44068056
Produced by FastQC (version 0.11.5)