FastQCFastQC Report
Wed 25 May 2016
SRR1294843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656611
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT188160.40407068574119676No Hit
GGTATCAACGCAGAGTACTTTTTTT118450.2543695404232821No Hit
TATCAACGCAGAGTACTTTTTTTTT110910.2381775072042737No Hit
GTACATGGGAAGCAGTGGTATCAAC100560.21595104250709368No Hit
CCCATGTACTCTGCGTTGATACCAC97930.21030315824104698No Hit
ATTCCATTCCATTCCATTCCATTCC66290.14235674828754216No Hit
GAATGGAATGGAATGGAATGGAATG56760.12189122089004215No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53870.11568499065092618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT400.00532890114.2306979
AGGACCG1052.6050657E-711.8024955
ACCGCCC750.002679225310.1196068
ATTTCGC750.002679225310.1196068
CGCCCGA750.002679702410.1193911
GGACCGC1002.7781515E-49.4929416
GTGTTAG7600.09.2848261
GACGCAA1356.973605E-69.13575610
AACACCG850.0072321058.9720325
CCGGTCG1856.730443E-88.7179049
ATAGGAC4450.08.5687953
ATAAGAC4500.08.4735863
TAGGACC3050.08.4388584
TACGATG1356.901222E-58.4341837
GCTAGAC2159.153155E-98.4243213
TCTATAC7250.08.4151473
GTCTTAG4200.08.40055751
TAAGACT5800.08.3822774
CGGTCGT1701.6451995E-68.37099710
GTCTTAC4450.08.357191
Produced by FastQC (version 0.11.5)