FastQCFastQC Report
Wed 25 May 2016
SRR1294842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140075
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79000.2515863474598537No Hit
CCCATGTACTCTGCGTTGATACCAC71910.22900726893465922No Hit
GAGTACATGGGAAGCAGTGGTATCA37400.11910543537972818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCG1103.83352E-812.08759913
GACGCTT1201.204462E-711.13019319
GCGACGT600.005886409411.0811838
CCGGCGT600.005886409411.0811838
TGTACCG1053.4693821E-610.8571175
CGCACGA700.001495546910.85417112
CGATTTC1158.8748675E-710.73447916
CGGGGAA1659.367795E-1010.35914716
TCTCGTA750.00255773610.17617719
TACGACC750.002648559910.133474
GTCTCGT1054.1173138E-59.9502929
CGTCCGC1252.5795507E-69.877768510
GCGTCGC1252.5874506E-69.87540514
GTATTAG3700.09.4999761
TAGCCCG800.0045177059.4999765
GTCGTAT1201.7016397E-59.4999761
TGGCGTC1701.6741978E-89.497412
TATACTG5550.09.2432195
TTTGACG1554.2909232E-79.18956616
GTATAGG3950.09.1392171
Produced by FastQC (version 0.11.5)