FastQCFastQC Report
Wed 25 May 2016
SRR1294842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140075
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98860.3148332444288751No Hit
GTACATGGGAAGCAGTGGTATCAAC81910.2608536420308432No Hit
CCCATGTACTCTGCGTTGATACCAC74920.23859302723661058No Hit
GGTATCAACGCAGAGTACTTTTTTT62490.19900798547805384No Hit
TATCAACGCAGAGTACTTTTTTTTT57910.1844223466000016No Hit
GAGTACATGGGAAGCAGTGGTATCA38650.1230862320167512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT701.0990193E-412.20540619
TACGTTA550.002997669212.1292715
TCGTCCG752.0996958E-411.3855459
TATACCG855.1574927E-511.2119315
CGCACGA600.00593298811.06945512
ACCGTTA803.817474E-410.6739488
CGTCAAA1355.7998477E-810.54233912
TGGCGTA856.407566E-410.0907375
ACGTCGC856.636323E-410.0523116
TTGCGAT856.674327E-410.0460699
ATACCGT1054.137165E-59.9459376
TAGACAG4250.09.8664995
TACCGCC1451.6463127E-79.8162197
GTACCGC1752.6066118E-99.7651036
GCACCGT1951.6552804E-109.7372826
GCGTAGG1002.6589466E-49.5342591
GCCGGTT800.00456094469.4890127
GCGCTGA3250.09.347796
CAGCGCA2357.2759576E-129.2860829
GTATTAA5050.09.2510621
Produced by FastQC (version 0.11.5)