FastQCFastQC Report
Wed 25 May 2016
SRR1294841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034213
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC56790.18716550222413522No Hit
CCCATGTACTCTGCGTTGATACCAC51900.1710492968028283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG1900.014.0017283
TTGCGTG2950.012.23071816
CGCAATC550.003077403612.08681112
CCGTCTG2550.011.91811859
ACCGTCT2250.011.81888
CGTCCGC2200.011.6559110
AGCGCGC1701.2732926E-1111.17342110
TCGTCCG2300.011.1489479
GGTACGA600.00587244211.0847012
TAGTACG2500.011.0213612
CTTGCGT3350.010.77033215
GGACCGA1355.7332727E-810.5505856
GTCCTAA2350.010.5119371
TTGAGCG750.002552561710.1786419
TCTATAC2900.010.1564263
CGTCGTA750.00265517910.1304019
GTACGGT1606.157279E-910.0959974
GTCCGCA2550.010.05541411
TCGTCTA1856.548362E-109.75151714
TGCGTGA3450.09.63218917
Produced by FastQC (version 0.11.5)