FastQCFastQC Report
Wed 25 May 2016
SRR1294841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034213
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69160.22793389916924092No Hit
GTACATGGGAAGCAGTGGTATCAAC56570.18644043776755292No Hit
CCCATGTACTCTGCGTTGATACCAC55720.18363905236712122No Hit
GGTATCAACGCAGAGTACTTTTTTT44420.14639710527902952No Hit
TATCAACGCAGAGTACTTTTTTTTT40200.13248905070276873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT802.0305142E-613.0455868
TACGTCG905.1955067E-712.7091155
ACGTCGC1103.862442E-812.0810266
CGACGTA802.906753E-511.8596239
ACCGTCT1900.010.9857558
TTAGAAC2100.010.8935263
GTCGCGC803.8183448E-410.6736618
GGTCTAA909.175667E-510.5940871
AAGACCG909.203442E-510.5909295
ACGGGTC909.203442E-510.5909295
CGTCCGC1803.6379788E-1110.54188710
AGACAAT5600.010.509266
TAGACGA2500.010.2943834
TTAAGAC2053.6379788E-1210.2292883
CGGACTG2250.010.1672925
GACGCTT1451.6376907E-79.81971519
TCTCGTA1554.118374E-89.79859719
TGGACCG1951.5279511E-109.7762425
TCGTCTA1856.6393113E-109.74428414
CGGGACT2650.09.7116824
Produced by FastQC (version 0.11.5)