FastQCFastQC Report
Wed 25 May 2016
SRR1294840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3499841
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC137050.39158921791018503No Hit
CCCATGTACTCTGCGTTGATACCAC123070.35164454613795315No Hit
GTATCAACGCAGAGTACTTTTTTTT70130.20038053157272No Hit
GAGTACATGGGAAGCAGTGGTATCA61940.17697946849585453No Hit
CATGTACTCTGCGTTGATACCACTG44170.126205733346172No Hit
GCGTTGATACCACTGCTTCCCATGT44020.12577714244732832No Hit
GTATCAACGCAGAGTACATGGGAAG42820.1223484152565788No Hit
GGTATCAACGCAGAGTACTTTTTTT41380.11823394262767938No Hit
TATCAACGCAGAGTACTTTTTTTTT39270.11220509731727814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA550.00308219412.08438614
CGAATTA1800.011.60484715
GTATTAG5850.010.5574181
CGTAGGC1659.385985E-1010.35878818
CCGAATT2250.010.12786714
GTATAAG5000.09.8817431
GTTCTAG3250.09.6478561
ACCGTCC900.00111668349.497738
ACCGTGT1606.6678695E-89.4977298
TTTCGTC1701.6816557E-89.49487517
TAACGTA1356.8812387E-69.14517713
ACGTAAT1356.900007E-69.14321315
TAACCCG1151.1088641E-49.08881955
AGGTTAC4500.09.075877
TCGCCAC1054.5193298E-49.04273814
ACGCCGA950.00183195338.9960466
AATCGTG850.00745147568.9392967
ATCGTAC850.00745497748.93878410
AACCCGT850.0074654918.9372486
CGCCAAA850.007471638.93635317
Produced by FastQC (version 0.11.5)