FastQCFastQC Report
Wed 25 May 2016
SRR1294840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3499841
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT235820.6738020384354604No Hit
GGTATCAACGCAGAGTACTTTTTTT145460.41561888097202127No Hit
GTACATGGGAAGCAGTGGTATCAAC138080.39453220874891176No Hit
TATCAACGCAGAGTACTTTTTTTTT136360.3896176997755041No Hit
CCCATGTACTCTGCGTTGATACCAC127610.36461656400962217No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65990.18855142276463416No Hit
GAGTACATGGGAAGCAGTGGTATCA63360.1810367956715748No Hit
CATGTACTCTGCGTTGATACCACTG46080.13166312412478165No Hit
GCGTTGATACCACTGCTTCCCATGT44820.12806296057449468No Hit
GTATCAACGCAGAGTACATGGGAAG44140.12612001516640328No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44010.12574856972073872No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39370.11249082458317393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG456.8348367E-414.75786416
ACCGTCC550.003101585712.074278
TAGGACC2250.011.8637534
AACGAAC700.001509268710.84235915
CGCCGAC700.001509628410.84204918
CGTAGGC2250.010.11924618
GTCTAGA2850.010.0374351
TACGTCT1159.85376E-69.9478664
GTATTAG5950.09.9362161
ATAGACG2500.09.9147083
CGCAAGT1151.0440866E-59.89954412
GCCCTAC1553.8402504E-89.8431631
CGCCAAA1651.0464646E-89.7746917
GTCTTAG3900.09.5355631
TATACCG800.00438899839.5333735
TATCCCG1106.583746E-59.5333725
ACACCGT900.00112219049.4926236
GCGGTTA800.00456889779.48706313
CCGTGTG900.00112836719.4869279
TACGCCA1651.0894473E-79.19957615
Produced by FastQC (version 0.11.5)