FastQCFastQC Report
Wed 25 May 2016
SRR1294839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4465768
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC151280.33875472259194833No Hit
CCCATGTACTCTGCGTTGATACCAC139490.31235388851368906No Hit
GAGTACATGGGAAGCAGTGGTATCA65070.14570842014184346No Hit
ATTCCATTCCATTCCATTCCATTCC63670.14257346104858112No Hit
CATGTACTCTGCGTTGATACCACTG52900.11845666859541291No Hit
GAATGGAATGGAATGGAATGGAATG50080.1121419652789845No Hit
GCGTTGATACCACTGCTTCCCATGT46830.10486438166962546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1302.6157068E-911.6936134
TAGACAG6150.010.9680895
TATACCG1053.4680415E-610.8577465
GCGTAAC750.002655414910.13048611
GTATTAA6150.010.0412081
GTCCTAA4350.09.609731
GCGCGTC900.00111655349.497979
ACGTTAC900.00111930619.49541316
AGGACCG1554.262638E-79.1940585
ATCCCGT1151.1164343E-49.08328156
TACGGGT1054.490623E-49.048634
CGCGCGT1054.5052526E-49.0456868
TAGCACC3700.08.987494
CTACACT7400.08.987494
GTATAGG5400.08.972721
GTACCGC1804.252979E-88.9685946
TCGACCG850.00745235858.9392659
CCTAGTA3100.08.8875892
AAGACCG1107.1449776E-48.6368445
TACCGCT1107.162545E-48.6347127
Produced by FastQC (version 0.11.5)