FastQCFastQC Report
Wed 25 May 2016
SRR1294839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4465768
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC148240.33194738284657865No Hit
CCCATGTACTCTGCGTTGATACCAC145540.3259013903095727No Hit
GTATCAACGCAGAGTACTTTTTTTT69500.1556283264155236No Hit
GAGTACATGGGAAGCAGTGGTATCA65720.1471639368637153No Hit
ATTCCATTCCATTCCATTCCATTCC61910.1386323696170513No Hit
CATGTACTCTGCGTTGATACCACTG53660.12015850353175533No Hit
GAATGGAATGGAATGGAATGGAATG51000.11420208125455689No Hit
GCGTTGATACCACTGCTTCCCATGT48060.10761866715870597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACT658.111929E-411.6779218
CGACGAC700.001501851410.8488866
GTCTACG1158.445113E-710.776231
TACGACC1002.3192222E-510.4829174
TACCGCT750.00267468710.1216587
AGCGTAG750.002676426210.12086510
ACGGGTC1053.9693165E-59.983735
ATACGAC1002.6716918E-49.5299243
TATTCCG2500.09.5299235
TCGACAC800.00440252679.5299233
GACGACC1002.7895402E-49.4890557
TAACACG1856.357368E-99.2723594
AGGACGG1451.6368813E-69.2013065
CTGGTCG1252.7969174E-59.1087789
TACGTCG950.0017733649.0283495
ACGTCGC950.00183752618.9931556
GTGTAAG9850.08.90374851
TCTACGG1856.2464096E-88.7582122
ACTGCGT1752.678662E-78.6750278
GTATTAG5950.08.6516331
Produced by FastQC (version 0.11.5)