FastQCFastQC Report
Wed 25 May 2016
SRR1294838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267824
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA60382.2544656192126173No Hit
GTACATGGGAAGCAGTGGTATCAAC56972.12714319851843No Hit
CCCATGTACTCTGCGTTGATACCAC53121.9833920783798316No Hit
GAGTACATGGGAAGCAGTGGTATCA31451.1742786307425772No Hit
CATGTACTCTGCGTTGATACCACTG30011.1205119780154131No Hit
GTATCAACGCAGAGTACTTTTTTTT27931.0428490351872872No Hit
GGTATCAACGCAGAGTACTTTTTTT21990.8210615926877352No Hit
GCGTTGATACCACTGCTTCCCATGT19440.7258498118167155No Hit
ACGCAGAGTACATGGGAAGCAGTGG19090.712781528167752No Hit
TATCAACGCAGAGTACTTTTTTTTT17290.6455732122587968No Hit
ACTCTGCGTTGATACCACTGCTTCC16450.6142093315012844No Hit
TATCAACGCAGAGTACATGGGAAGC14480.5406535635342613No Hit
GGTATCAACGCAGAGTACATGGGAA14390.5372931477388135No Hit
GTGGTATCAACGCAGAGTACATGGG13480.5033156102515085No Hit
AAACAAAAAAAAAAAAAAAAAAAAA12860.4801660792162017No Hit
GTATCAACGCAGAGTACATGGGAAG12840.4794193201505466No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11170.4170649381683494No Hit
GTACTCTGCGTTGATACCACTGCTT10160.37935360535276896No Hit
GCTTCCCATGTACTCTGCGTTGATA9970.3722593942290459No Hit
CAGTGGTATCAACGCAGAGTACATG9700.36217814684270266No Hit
ATTTAGGGCTGCATTCCCAAACAAC9390.3506033813250493No Hit
GCAGAGTACATGGGAAGCAGTGGTA8840.3300675070195352No Hit
ATACCACTGCTTCCCATGTACTCTG8810.3289473684210526No Hit
GTACTTGTTCGCTATCGGTCTCTCG8800.3285739888882251No Hit
CTGCTTCCCATGTACTCTGCGTTGA8470.31625246430491666No Hit
CATGGGAAGCAGTGGTATCAACGCA8190.3057978373857459No Hit
GATACCACTGCTTCCCATGTACTCT8070.30131728299181554No Hit
GTTGATACCACTGCTTCCCATGTAC7720.28824899934285203No Hit
GTATTTAGCCTTAGATGGAATTTAC7660.28600872214588685No Hit
GGGAAGCAGTGGTATCAACGCAGAG7600.28376844494892167No Hit
GCAGTGGTATCAACGCAGAGTACAT7590.28339506541609416No Hit
CTGCTACTCCACGAGGCGCTGTCGC6890.25725849811816714No Hit
TTACTAAGCGGAGGAAAAGAAACTA6690.24979090746161656No Hit
AAGCAGTGGTATCAACGCAGAGTAC6660.24867076886313397No Hit
CCACTGCTTCCCATGTACTCTGCGT6560.2449369735348587No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6470.24157655773941095No Hit
GAGTAGCAGGGTCCGGGGTCGACGG6400.23896290100961826No Hit
TACCACTGCTTCCCATGTACTCTGC6270.2341089670828604No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6250.23336220801720536No Hit
AAAAACAAAAAAAAAAAAAAAAAAA6170.2303751717545851No Hit
GTCCGCTACAGAGGACGCTTCTTTA6000.22402771969651714No Hit
CTTCCCATGTACTCTGCGTTGATAC5660.21133281558038114No Hit
TTCCCAAACAACCCGACTCGGCGAC5600.209092538383416No Hit
ACCCCGGACCCTGCTACTCCACGAG5530.2064788816536233No Hit
ACATGGGAAGCAGTGGTATCAACGC5430.20274508632534802No Hit
TCTTTAGACTACAATTCGGACAGCG5400.20162494772686543No Hit
ATGGGAAGCAGTGGTATCAACGCAG5150.1922904594061772No Hit
CGCCAGTATTTAGCCTTAGATGGAA4940.1844494892167991No Hit
GTCCGGGGTCGACGGGGCTCTCACC4810.17959555529004123No Hit
GTAGCGGACCGGGCCCAAGTTCCCT4700.1754883804289384No Hit
GCATATTACTAAGCGGAGGAAAAGA4680.17474162136328336No Hit
ACCGGGAAGAGCCCAACTTGAAAAT4570.17063444650218054No Hit
CTATCGGTCTCTCGCCAGTATTTAG4570.17063444650218054No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4560.170261066969353No Hit
CCGCTACAGAGGACGCTTCTTTAGA4470.16690065117390526No Hit
TGATACCACTGCTTCCCATGTACTC4400.16428699444411254No Hit
GTCTAAAGAAGCGTCCTCTGTAGCG4300.16055319911583726No Hit
CTACAGAGGACGCTTCTTTAGACTA4270.15943306051735467No Hit
GCCCTAAATGGGTGGTAAATTCCAT4260.15905968098452714No Hit
GGTGAGAGCCCCGTCGACCCCGGAC4190.15644602425473444No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4150.15495250612342434No Hit
CGGTACTTGTTCGCTATCGGTCTCT4140.1545791265905968No Hit
CCCCTAGTAACGGCGAGCGAACCGG3870.14449787920425355No Hit
GTGGTATCAACGCAGAGTACTTTTT3870.14449787920425355No Hit
CCTCAGATCAGACAAGATTACCCGC3780.14113746340880579No Hit
TTCCCATGTACTCTGCGTTGATACC3700.13815042714618556No Hit
GCCTTAGATGGAATTTACCACCCAT3630.13553677041639284No Hit
GCTCTCACCCTCTCTGACGTCCCAT3620.13516339088356533No Hit
CTGTAGCGGACCGGGCCCAAGTTCC3560.13292311368660015No Hit
ACACAAAAAAAAAAAAAAAAAAAAA3490.13030945695680746No Hit
GTACATGGGCATTGACCTCAGATCA3460.12918931835832487No Hit
GGATTCCCCTAGTAACGGCGAGCGA3460.12918931835832487No Hit
AGATTACCCGCCGAATTTAAGCATA3450.12881593882549733No Hit
ACAGAGGACGCTTCTTTAGACTACA3420.12769580022701477No Hit
CCATGTACTCTGCGTTGATACCACT3360.1254555230300496No Hit
GTTTGGGAATGCAGCCCTAAATGGG3340.12470876396439452No Hit
GGTATCAACGCAGAGTACATGGGGG3300.12321524583308442No Hit
CTTCCCGGTTCGCTCGCCGTTACTA3240.12097496863611924No Hit
CCTCTGTAGCGGACCGGGCCCAAGT3230.12060158910329172No Hit
GGTATCAACGCAGAGTACATGGGCA3200.11948145050480913No Hit
GATCAGACAAGATTACCCGCCGAAT3040.11350737797956867No Hit
TAGTAACGGCGAGCGAACCGGGAAG2910.1086534440528108No Hit
GAATTTACCACCCATTTAGGGCTGC2900.10828006451998326No Hit
GGAAGCAGTGGTATCAACGCAGAGT2850.10641316685584562No Hit
CCCATTTAGGGCTGCATTCCCAAAC2820.10529302825736304No Hit
GTCCTCTGTAGCGGACCGGGCCCAA2790.10417288965888045No Hit
ATCAACGCAGAGTACTTTTTTTTTT2770.1034261305932254No Hit
ATCAACGCAGAGTACATGGGAAGCA2750.10267937152757035No Hit
GTACATGGTAAGCAGTGGTATCAAC2720.10155923292908776No Hit
CCGTTACTAGGGGAATCCTTGTTAG2710.10118585339626023No Hit
CCCTGCTACTCCACGAGGCGCTGTC2680.10006571479777764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAA250.00600593119.0073071
TAACAAC402.7423282E-416.6313921
GACCAAA350.002158322816.2919771
GAACAAA2850.014.6723061
GAGAACA655.4000444E-513.1589051
GCATATT1103.7407517E-812.0955591
CGGACCC1302.568413E-911.6924245
AGTAGCA1552.5465852E-1111.6496392
CCGGACC1354.614776E-911.2635894
ACCGATA951.2879147E-511.04561419
GGAAACA700.001479650310.8613181
GAGTAGC1605.275069E-1010.6916091
CATATTA1252.1975029E-710.6440912
AGAAACT1252.226061E-710.634130518
GAAAACA1553.5652192E-910.4233621
TAAGCAT1106.0232323E-610.3579218
ATATTAC1303.768564E-710.2347043
AGAACAA750.002626913110.137232
GTACTTA750.002626913110.137231
GGACCCT1502.4288966E-810.1258496
Produced by FastQC (version 0.11.5)