##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294838_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267824
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.890569926518907	33.0	31.0	34.0	16.0	34.0
2	30.057429505944203	33.0	31.0	34.0	16.0	34.0
3	30.42085100663122	34.0	31.0	34.0	23.0	34.0
4	33.142911016189736	37.0	35.0	37.0	18.0	37.0
5	34.047161568791445	37.0	35.0	37.0	28.0	37.0
6	33.852096899456356	37.0	35.0	37.0	26.0	37.0
7	33.96526450206105	37.0	35.0	37.0	27.0	37.0
8	33.84375186689766	37.0	35.0	37.0	25.0	37.0
9	35.01706344465022	39.0	37.0	39.0	20.0	39.0
10	35.29385715992592	39.0	37.0	39.0	25.0	39.0
11	35.19301108190454	39.0	37.0	39.0	23.0	39.0
12	35.35022253420156	39.0	37.0	39.0	25.0	39.0
13	34.992147828424635	39.0	37.0	39.0	21.0	39.0
14	36.34733631041281	41.0	38.0	41.0	22.0	41.0
15	36.42391645259573	41.0	38.0	41.0	24.0	41.0
16	36.420608309934885	40.0	38.0	41.0	25.0	41.0
17	36.50333801302348	40.0	38.0	41.0	25.0	41.0
18	36.57220040026286	41.0	37.0	41.0	26.0	41.0
19	36.639815699862595	40.0	37.0	41.0	27.0	41.0
20	36.70531767130653	41.0	37.0	41.0	27.0	41.0
21	36.69007258498118	41.0	37.0	41.0	27.0	41.0
22	36.74388404325229	41.0	37.0	41.0	27.0	41.0
23	36.76838894199176	40.0	37.0	41.0	27.0	41.0
24	36.917337505227316	41.0	36.0	41.0	27.0	41.0
25	36.033764711153594	40.0	34.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13306864916200567
1101	2	-0.016062381421445338
1101	3	-0.21012464905594896
1101	4	-0.23298840183618807
1101	5	-0.29020080541759086
1101	6	-0.432142133305625
1101	7	-0.23651165040782018
1101	8	-0.42973031393493244
1101	9	-0.028897198300185778
1101	10	-0.27007411976750006
1101	11	-0.25537393764907534
1101	12	0.12447531697836922
1101	13	-0.2128775732730901
1101	14	0.21698481448165552
1101	15	0.2101821421279979
1101	16	0.251009177162679
1101	17	-0.14771447898671397
1101	18	-0.15291814724495367
1101	19	0.06365859605163848
1101	20	-0.2938888258321626
1101	21	-0.07646036503652454
1101	22	-0.13360211977608571
1101	23	-0.23161869511726962
1101	24	-0.24098987450099685
1101	25	-0.5244072299755942
1102	1	0.09093381770133036
1102	2	-0.257635415129311
1102	3	-0.013495435572803416
1102	4	0.008584631871677573
1102	5	-0.059863726765904346
1102	6	0.1577455071438152
1102	7	-0.13819704366624563
1102	8	0.28656182089652305
1102	9	0.0637994309132992
1102	10	0.4490270038280002
1102	11	-0.0671716904580606
1102	12	0.04020565405701859
1102	13	0.023077482906685987
1102	14	0.236647735829969
1102	15	0.32535068145384116
1102	16	-0.04393464306203754
1102	17	-0.31344481606536334
1102	18	-0.3298844393797893
1102	19	-0.4925211792292643
1102	20	-0.03827084830407301
1102	21	-0.04556148863203191
1102	22	-0.17854594000080226
1102	23	-0.3804950996116503
1102	24	-0.027506728433579042
1102	25	-0.22665442098682576
1103	1	0.16981496978235455
1103	2	0.4235799670514133
1103	3	0.16798897651771583
1103	4	0.31059444355074106
1103	5	-0.13876070413574126
1103	6	0.1690447633552452
1103	7	0.25006850468000863
1103	8	0.3236642105151333
1103	9	0.06701987718644631
1103	10	0.3836606483920164
1103	11	0.6995556398157134
1103	12	0.061095035288225574
1103	13	0.17264228746437027
1103	14	0.22717630047627324
1103	15	0.18126933946934543
1103	16	0.49536746170137036
1103	17	0.05624975586057701
1103	18	0.389192946282499
1103	19	0.271373424347253
1103	20	0.3834810111673619
1103	21	0.5253595431768048
1103	22	0.46253650975390315
1103	23	0.5418660334790246
1103	24	0.6427971171115985
1103	25	0.5137000654825528
1104	1	-0.5916504519615913
1104	2	-0.6340399095113334
1104	3	-0.6061920647862884
1104	4	-0.7807412108249565
1104	5	-0.708740131260285
1104	6	-0.7692207849910133
1104	7	-1.003365582992089
1104	8	-0.6656853701147085
1104	9	-0.8575488836934397
1104	10	-1.2391752433630074
1104	11	-1.2806548365254784
1104	12	-0.897996593133989
1104	13	-0.780293303610172
1104	14	-1.4178466461925012
1104	15	-1.0426268466360469
1104	16	-1.4091031823878808
1104	17	-1.2319841419080575
1104	18	-1.360783315784282
1104	19	-1.5515099432742048
1104	20	-1.4624281516748638
1104	21	-1.7562356459353978
1104	22	-1.5549504343828247
1104	23	-1.3411692569150233
1104	24	-1.2662707733774
1104	25	-1.4569914996385123
1105	1	-0.21002820536350697
1105	2	-0.2658770919251374
1105	3	-0.23465697836726562
1105	4	-0.08352648253347184
1105	5	-0.3126504807903956
1105	6	-0.3838334558279328
1105	7	-0.08064612611266142
1105	8	-0.12489203460731346
1105	9	-0.2588785211189517
1105	10	0.10790904112307231
1105	11	0.029586484514510403
1105	12	-0.027239722599468053
1105	13	-0.21754686858007943
1105	14	0.27710755024401124
1105	15	0.025678273283311626
1105	16	0.4193854482486259
1105	17	0.2682011475882504
1105	18	0.3448732019590395
1105	19	0.15075207089673626
1105	20	-0.053056950109535705
1105	21	0.12179939601413992
1105	22	0.007632945890513554
1105	23	-0.14059708295042128
1105	24	0.033097760019174416
1105	25	-0.3691198089089198
1106	1	0.2552199446185206
1106	2	-0.029970125276332027
1106	3	0.019692513937609846
1106	4	0.2835333624109424
1106	5	-0.3290717219133583
1106	6	0.01026276545524496
1106	7	-0.018739200160858616
1106	8	-0.26010783047114217
1106	9	-0.1720039831995237
1106	10	0.23763074144390117
1106	11	-0.07028396335601883
1106	12	0.12061927584726106
1106	13	0.11009114513480966
1106	14	-0.09250942311980026
1106	15	-0.29183975209804913
1106	16	-0.0977242505257152
1106	17	0.002822767193350728
1106	18	0.40535573277006165
1106	19	-0.05299747974051172
1106	20	0.008456571470048857
1106	21	0.10780640946450148
1106	22	0.12921524540689688
1106	23	0.20186820589656662
1106	24	-0.1113359072556861
1106	25	0.10431522171558072
1107	1	-0.4856846948722371
1107	2	-0.4938579940485184
1107	3	-0.699985537231445
1107	4	-0.6120413713385986
1107	5	-0.4395319985690094
1107	6	-0.517776162198075
1107	7	-0.5272286113494999
1107	8	-0.827643636567359
1107	9	-0.6342209008148956
1107	10	-0.3602025306824288
1107	11	-0.54108822336336
1107	12	-0.95409611159306
1107	13	-0.9057614684041795
1107	14	-1.1676593802748982
1107	15	-0.8852967235755855
1107	16	-1.11046754835899
1107	17	-1.181208325963702
1107	18	-0.9190230161588175
1107	19	-1.113200686987419
1107	20	-0.8601809606636266
1107	21	-0.7344325426716196
1107	22	-0.9651965553031019
1107	23	-0.9496497277550446
1107	24	-0.7734671350681452
1107	25	-0.8239256890446143
1108	1	-0.2201275982471813
1108	2	-0.26142618637399195
1108	3	0.2925479810302285
1108	4	-0.09082084429282844
1108	5	0.3223496472932226
1108	6	0.28485906665074623
1108	7	0.16830999244152878
1108	8	-0.19789328982672316
1108	9	0.2530652757916698
1108	10	-0.11047992319283395
1108	11	0.10142217885577764
1108	12	-0.009019828652697015
1108	13	0.2236883697835239
1108	14	0.09415291745973065
1108	15	0.22422708594822183
1108	16	0.2004201076742973
1108	17	-0.1521052284140083
1108	18	0.1743426796340657
1108	19	0.3418848423699501
1108	20	0.11117826263734543
1108	21	-0.021071470087250077
1108	22	-0.0880583213687558
1108	23	0.1254774104788865
1108	24	0.04132713215330597
1108	25	0.3479883192771638
1109	1	0.05215360591570217
1109	2	0.14351781958628962
1109	3	0.14946632984632302
1109	4	0.034909639281480054
1109	5	-0.30803148469439634
1109	6	-0.12687174287638925
1109	7	0.11866039173374787
1109	8	0.06725187559433721
1109	9	0.04524124375516436
1109	10	0.4814213130996876
1109	11	0.3849138321350978
1109	12	0.1976168037889181
1109	13	-0.24269192403966855
1109	14	0.02346771804644021
1109	15	-0.1900414991836712
1109	16	0.4102929860706084
1109	17	0.3370684811620208
1109	18	0.09068921405701502
1109	19	0.1618250067433067
1109	20	-0.007183358745159296
1109	21	-0.2692903706949181
1109	22	-0.2760050513633985
1109	23	-0.367858018265494
1109	24	-0.21798580584580662
1109	25	0.1465436497700523
1110	1	-0.013491799238234847
1110	2	0.09566141648705795
1110	3	0.16461910370114197
1110	4	0.12304605855252504
1110	5	-0.007556852852523832
1110	6	0.386694309593409
1110	7	0.15917301627614222
1110	8	0.25378163562003664
1110	9	0.3223885790047447
1110	10	-0.1426835600456755
1110	11	-0.30618225173683555
1110	12	9.187349922257226E-4
1110	13	-0.06780919742649871
1110	14	-0.02223982677220704
1110	15	-0.036165523751549244
1110	16	0.06501908971425507
1110	17	-0.36333193753304727
1110	18	-0.14508152270230568
1110	19	-0.05616780455038395
1110	20	-0.1851232239430729
1110	21	-0.11975641818025196
1110	22	-0.16928211562423456
1110	23	-0.1997597024608666
1110	24	-0.22324718565651125
1110	25	0.08838048053637948
1111	1	0.6920209328333904
1111	2	0.9801679799774554
1111	3	0.8041270168811074
1111	4	0.9054910961938702
1111	5	1.1455329364959255
1111	6	0.7374186429967295
1111	7	0.8457151115942239
1111	8	1.0481139391902161
1111	9	1.158636242042249
1111	10	0.7709663004863998
1111	11	0.5931109108357759
1111	12	0.8301030226060746
1111	13	0.8323337696910613
1111	14	0.7938124811969658
1111	15	0.7339673456884839
1111	16	0.8782770403855977
1111	17	0.8822115874562115
1111	18	0.9950462479104516
1111	19	0.9807387069519748
1111	20	1.1207714202887544
1111	21	1.0393769811378988
1111	22	1.3302579901514378
1111	23	0.8371468090414922
1111	24	0.9042384926374822
1111	25	0.7202301551112313
1112	1	-0.06409504421282008
1112	2	-0.025671142140737402
1112	3	0.2825826697553673
1112	4	0.2285052913010901
1112	5	0.22763300696809097
1112	6	0.07885627513203275
1112	7	-0.36992970870176123
1112	8	-0.3520169376848443
1112	9	-0.09425815038702723
1112	10	-0.2528935605827627
1112	11	0.14420322936994978
1112	12	-0.12182489443812017
1112	13	-0.1844788750285602
1112	14	-0.46230349584924824
1112	15	-0.2699589605634074
1112	16	-0.09449050214938381
1112	17	0.0031216851104929333
1112	18	0.08051891477846596
1112	19	-0.05142680195772442
1112	20	-0.0751915314881515
1112	21	0.05759906912743418
1112	22	-0.13702279108282767
1112	23	0.03753943966668061
1112	24	-0.1883317278397172
1112	25	-0.10112291042788257
1113	1	0.22400593297125582
1113	2	0.21875953704035211
1113	3	0.6720648115704968
1113	4	0.4638912947214564
1113	5	0.6513233244519228
1113	6	0.3333460701343469
1113	7	0.22710866496229443
1113	8	0.15767635130040247
1113	9	0.054298965730446014
1113	10	0.0022785126903315245
1113	11	0.19473221946345376
1113	12	0.013010348825503115
1113	13	0.22481178000346347
1113	14	0.34635335857860383
1113	15	0.46361137469556724
1113	16	0.19171625509869727
1113	17	0.3282093264475776
1113	18	0.29360968023184597
1113	19	0.23147629208446574
1113	20	0.5243201009075023
1113	21	0.5258798625452386
1113	22	0.6405814246786932
1113	23	0.7927676213502011
1113	24	0.30549859134981006
1113	25	0.22351137495296314
1114	1	-0.29263669410880766
1114	2	-0.06304398507819542
1114	3	-0.26210501428078103
1114	4	-0.17684240197129242
1114	5	-0.1516371612753744
1114	6	-0.6250335340847073
1114	7	-0.5516250232208648
1114	8	-0.20743178429137998
1114	9	-0.6846694168946783
1114	10	-0.5904057943526269
1114	11	-0.8285202302342469
1114	12	-0.2252240818192277
1114	13	-0.5994822432872766
1114	14	-0.6354616514839861
1114	15	-0.9866947577391514
1114	16	-0.44608163367247755
1114	17	-0.20632045498364704
1114	18	0.006862087381598769
1114	19	-0.04985494789017508
1114	20	-0.19905871617464754
1114	21	0.11113955390247554
1114	22	0.3253607497832576
1114	23	0.41176357504102157
1114	24	0.4394778024258912
1114	25	0.35632051765450257
1115	1	0.08812482893728557
1115	2	0.20393669759863187
1115	3	0.004210403439905974
1115	4	0.28075722350917687
1115	5	0.10430094444426175
1115	6	0.13216528979255315
1115	7	0.29802509669109156
1115	8	0.24226268661765005
1115	9	0.36521313509454956
1115	10	0.012689740980334818
1115	11	-0.058652626173667954
1115	12	0.1183277759947643
1115	13	0.17552268460129028
1115	14	0.21376138475242357
1115	15	0.19144020070231704
1115	16	0.5161132478939336
1115	17	0.48596575678414666
1115	18	0.40788955214719635
1115	19	0.31669782979633965
1115	20	0.4406387114675212
1115	21	0.3368984487413371
1115	22	0.7269384954385032
1115	23	0.5091715056563544
1115	24	0.39577564401069765
1115	25	0.8286504233675629
1116	1	-0.21782999652472768
1116	2	-0.24926036033384236
1116	3	-0.06674509526410333
1116	4	-0.01598680988362844
1116	5	0.034805539320892365
1116	6	-0.38326457522242663
1116	7	-0.15111147044796525
1116	8	-0.3734928592132931
1116	9	-0.6379805566279373
1116	10	-0.48965398272115124
1116	11	-0.3765238349796718
1116	12	-0.24000106905859298
1116	13	-0.24080595847946995
1116	14	-0.4892884014932406
1116	15	-0.3943560762694389
1116	16	-0.2867362488576717
1116	17	-0.2141554267752852
1116	18	0.29086746980969735
1116	19	-0.1350643811303769
1116	20	0.09402848492158711
1116	21	-0.3262238793294898
1116	22	-0.029756054667529952
1116	23	0.24495768399815887
1116	24	-0.03054643496058418
1116	25	0.07070616780490013
1201	1	0.17797990140105213
1201	2	0.07032420056846078
1201	3	-0.4317660755337229
1201	4	0.2588696017462908
1201	5	-0.11810342714043998
1201	6	0.21897332020358107
1201	7	0.11159126442689882
1201	8	-0.10217779805804383
1201	9	-0.1519227641689369
1201	10	0.22799622708154743
1201	11	0.014191279742227891
1201	12	0.027373867703005317
1201	13	0.031919691016113916
1201	14	-0.003891264332871458
1201	15	0.10611114391923593
1201	16	0.37338338331802845
1201	17	0.3058517154441631
1201	18	-0.022646204564608752
1201	19	0.2843148409628924
1201	20	0.2340219378870998
1201	21	0.13254468300125666
1201	22	-0.009192414516355996
1201	23	0.08854903000892733
1201	24	0.19433824795462584
1201	25	-0.06988842196386713
1202	1	0.33605871323480585
1202	2	0.384733111815315
1202	3	0.6366732585182611
1202	4	0.3681042406251933
1202	5	0.3920894103251271
1202	6	0.47984288542097175
1202	7	0.46554982233802633
1202	8	0.6441391261244931
1202	9	0.5706787449938417
1202	10	0.6458187207933648
1202	11	0.6041301945823321
1202	12	0.8448609919094991
1202	13	0.9000236555627623
1202	14	0.7505224379069162
1202	15	0.5899295652870649
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2210	7	0.23986206053569248
2210	8	0.12206282135827706
2210	9	0.22191086696070528
2210	10	0.08102382287268739
2210	11	0.02773108518197631
2210	12	0.11305979186421666
2210	13	0.037160906025043516
2210	14	0.09215797132327452
2210	15	0.1660079078660317
2210	16	0.5449140563376815
2210	17	0.35822415935918883
2210	18	0.09170757744848856
2210	19	0.2586845054457143
2210	20	0.6690427836286119
2210	21	0.5834082922925035
2210	22	0.636727776073279
2210	23	0.49180493630235844
2210	24	0.5443084683238979
2210	25	0.4178225170871528
2211	1	0.2937323055727994
2211	2	0.6281936303395561
2211	3	0.35159434705871817
2211	4	0.1922977474864993
2211	5	0.4766006227037991
2211	6	0.2796871221810946
2211	7	0.25884170462735767
2211	8	0.5194191137664177
2211	9	0.4392325927320542
2211	10	0.3526813144444034
2211	11	0.30070082870606285
2211	12	0.318400550623231
2211	13	0.8177587808170088
2211	14	0.6457362583543826
2211	15	0.768619609312708
2211	16	0.3916686332164403
2211	17	0.5431129913482664
2211	18	0.5705408467695321
2211	19	0.7734926819489374
2211	20	0.6276695066260913
2211	21	0.7167245556817363
2211	22	0.5432591634329853
2211	23	0.35186340086088563
2211	24	0.41416081330746835
2211	25	0.2512412001409672
2212	1	0.3803326211450795
2212	2	0.5407740405835177
2212	3	0.29979801255210603
2212	4	0.5223742130769864
2212	5	0.4204247113877031
2212	6	0.06425846983655248
2212	7	0.3543731202522338
2212	8	0.4810143720906481
2212	9	0.22790031130631405
2212	10	0.02204855156865193
2212	11	0.25977757750244024
2212	12	0.5075922000952531
2212	13	0.5228099601672511
2212	14	0.19208087946535812
2212	15	0.18201929374043146
2212	16	-0.4637143016057408
2212	17	-0.16540746016283947
2212	18	-0.0027892499250512515
2212	19	-0.1404734142601498
2212	20	0.17271947223680684
2212	21	0.2904592322201509
2212	22	0.11164327153672104
2212	23	0.0016489735436522324
2212	24	0.13584022423921738
2212	25	0.19642063130170584
2213	1	-0.4871889785940944
2213	2	0.051071274730123406
2213	3	0.10974747731328449
2213	4	-0.07519140122502677
2213	5	0.044797549149663496
2213	6	0.5063421880709029
2213	7	0.17188475784755042
2213	8	0.45412564018099744
2213	9	-0.12757615086470508
2213	10	-0.13252769505780293
2213	11	-0.12566682467836188
2213	12	0.28765523329636267
2213	13	0.2392873461007028
2213	14	-0.05580442908940597
2213	15	0.198076458333162
2213	16	-0.06312744076783616
2213	17	-0.23911826818326887
2213	18	-0.07934379719089435
2213	19	-0.18765775014741592
2213	20	-0.23716840836237196
2213	21	-0.04051941256602021
2213	22	0.0635442297138269
2213	23	0.1771185527789214
2213	24	0.2552138770856729
2213	25	0.4119380288274712
2214	1	0.8206597590770066
2214	2	0.75920730392696
2214	3	0.7331909669031447
2214	4	0.5535845197363969
2214	5	0.4256091477098636
2214	6	0.6514995720477188
2214	7	0.7041799999992335
2214	8	0.40064264800635385
2214	9	1.0323174510527977
2214	10	0.5003905688328274
2214	11	0.5550053038678939
2214	12	0.668387492627069
2214	13	0.899627753152707
2214	14	0.9403246516612285
2214	15	0.9492769861478223
2214	16	1.0666924174237309
2214	17	0.7304112919433408
2214	18	0.7070248880328052
2214	19	0.5344753894311722
2214	20	0.31766335071362306
2214	21	0.41120475416327906
2214	22	0.19127621344511425
2214	23	0.2711230124787747
2214	24	0.3579414762805868
2214	25	0.28482823168535276
2215	1	0.612975723569491
2215	2	0.6007647457034473
2215	3	0.8338921490319287
2215	4	0.6718410484285116
2215	5	0.46744991944670744
2215	6	0.5878614407556313
2215	7	0.7134179852613087
2215	8	0.5506011802460478
2215	9	0.9225290755175877
2215	10	0.8290334818140224
2215	11	0.887767215432433
2215	12	0.3779544422268799
2215	13	0.5776712685076859
2215	14	0.6884705070677128
2215	15	0.5994755948945425
2215	16	0.7702197563839803
2215	17	0.8505487506243838
2215	18	0.8906854000108311
2215	19	0.6429860024000646
2215	20	0.8004552222389592
2215	21	0.8142515432888047
2215	22	0.911140659503296
2215	23	0.6982012615472328
2215	24	0.6011092163471687
2215	25	0.9156937372547347
2216	1	-0.08776449912595652
2216	2	-0.027831267152226502
2216	3	-0.0432285261266081
2216	4	-0.28987875134988883
2216	5	0.001922923320698544
2216	6	-0.18352997360406675
2216	7	0.07101155428196648
2216	8	0.06975896083523736
2216	9	-0.12096097233757774
2216	10	0.07305551561909596
2216	11	-0.047864056226153195
2216	12	-0.12848910989909967
2216	13	-0.27598544732380503
2216	14	-0.3278701839639666
2216	15	-0.00996263737193459
2216	16	-0.27483796055548737
2216	17	0.265140252993568
2216	18	0.1802523461600245
2216	19	-0.06640979671826841
2216	20	0.14890994842728134
2216	21	0.23399395836357684
2216	22	0.36761917968654245
2216	23	0.13782439232777222
2216	24	0.23992454571484245
2216	25	0.13129734878712185
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	245.0
3	41.0
4	28.0
5	18.0
6	20.0
7	70.0
8	30.0
9	79.0
10	163.0
11	273.0
12	469.0
13	891.0
14	1497.0
15	2497.0
16	3442.0
17	4265.0
18	4387.0
19	4162.0
20	3803.0
21	3450.0
22	2959.0
23	2560.0
24	2205.0
25	2025.0
26	1717.0
27	1629.0
28	1438.0
29	1389.0
30	1653.0
31	2212.0
32	2458.0
33	3221.0
34	4378.0
35	6897.0
36	12457.0
37	28717.0
38	160079.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.22191809467619	24.011844792884162	9.914599491405804	22.851637621033845
2	14.931737422853935	28.986721526089397	30.540489994389375	25.541051056667293
3	19.617854885105842	35.26612876075905	23.841052170887995	21.27496418324711
4	13.147116798013158	24.422966872257902	27.548352589589282	34.88156374013967
5	12.365233395931376	44.26068219397413	28.137321651690524	15.236762758403973
6	27.824880414985248	39.40751655864434	15.229838845401538	17.537764180968875
7	21.65437381248036	41.488008856839365	18.35101210334974	18.50660522733053
8	24.801447823030557	39.31220927623805	18.137423533106986	17.748919367624403
9	24.105005684878225	23.888456705164263	16.977215606486745	35.02932200347077
10	16.56399495805234	36.836514473157614	25.149519182217034	21.449971386573008
11	28.258073201011115	32.735913965628136	15.653933021224404	23.35207981213635
12	20.775665740494897	36.83009921132049	22.371722716886865	20.02251233129775
13	27.588799730654845	29.89806034079644	19.789761143220545	22.723378785328173
14	21.006907435871543	33.05957897177563	21.690919358397565	24.242594233955263
15	28.064329125759702	31.255726975222064	20.787283777466108	19.892660121552126
16	19.42999465222643	33.953126577686696	24.843213002195206	21.773665767891668
17	22.244619383309335	33.724265599581145	22.844854989809086	21.186260027300435
18	21.819487348257628	29.95729148746793	26.405229735895347	21.817991428379095
19	22.024245778302944	33.23287015570546	27.195731187459803	17.54715287853179
20	22.316930407561028	30.504430173803442	25.199067961746035	21.97957145688949
21	22.080719903952964	30.374127142638073	26.48063163642756	21.0645213169814
22	21.51224746955426	31.425013928201523	22.919276256996817	24.1434623452474
23	20.905313471240984	30.56657827711434	25.61028061501176	22.91782763663292
24	22.201693495302496	34.262985458679914	23.74970079588295	19.78562025013464
25	22.10858154136341	30.41846239399416	23.313581898317057	24.15937416632537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6508.0
1	6508.0
2	5655.5
3	4803.0
4	4803.0
5	4803.0
6	4522.5
7	4242.0
8	4242.0
9	4242.0
10	4189.0
11	4136.0
12	4136.0
13	4136.0
14	3856.5
15	3577.0
16	3577.0
17	3577.0
18	4160.5
19	4744.0
20	4744.0
21	4744.0
22	5725.5
23	6707.0
24	6707.0
25	6707.0
26	8077.0
27	9447.0
28	9447.0
29	9447.0
30	11265.5
31	13084.0
32	13084.0
33	13084.0
34	13915.5
35	14747.0
36	14747.0
37	14747.0
38	15042.0
39	15337.0
40	15337.0
41	15337.0
42	18485.5
43	21634.0
44	21634.0
45	21634.0
46	36002.0
47	50370.0
48	50370.0
49	50370.0
50	45864.5
51	41359.0
52	41359.0
53	41359.0
54	32141.5
55	22924.0
56	22924.0
57	22924.0
58	18935.0
59	14946.0
60	14946.0
61	14946.0
62	12606.0
63	10266.0
64	10266.0
65	10266.0
66	10207.0
67	10148.0
68	10148.0
69	10148.0
70	7497.0
71	4846.0
72	4846.0
73	4846.0
74	4025.0
75	3204.0
76	3204.0
77	3204.0
78	1937.5
79	671.0
80	671.0
81	671.0
82	388.5
83	106.0
84	106.0
85	106.0
86	61.5
87	17.0
88	17.0
89	17.0
90	8.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01008124738634327
2	0.17698189856024854
3	0.1833293506183165
4	0.17362148276480077
5	0.19042356174203953
6	0.16466037397694008
7	0.17175458510066313
8	0.14487125873708107
9	0.16727403070673277
10	0.17436824183045582
11	0.1478582949997013
12	0.15495250612342434
13	0.19005018220921202
14	0.16092657864866478
15	0.1676474102395603
16	0.15793954238604455
17	0.16017981958300972
18	0.16055319911583726
19	0.1478582949997013
20	0.1691409283708704
21	0.1698876874365255
22	0.14225760200728838
23	0.16690065117390526
24	0.16727403070673277
25	0.6283977537487305
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	267824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.02355278093076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4943750353327	24.930924786426907
2	12.76838713324665	8.43315012844256
3	4.615297642602748	4.572405759005915
4	2.2386794052801173	2.957165899994026
5	1.1600429645542427	1.9154370034052213
6	0.7292667759624626	1.4449787920425354
7	0.4669568658488326	1.079440229404385
8	0.33240997229916897	0.8781886612103471
9	0.22273729436372885	0.6620019117032081
>10	1.4879303521962801	9.51781767130653
>50	0.22386794052801176	5.3255122767190395
>100	0.20690824806376845	13.959540593822808
>500	0.03278873876420374	7.764054005615629
>1k	0.016959692464243316	10.194381384790011
>5k	0.003391938492848663	6.365000896110878
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	6038	2.2544656192126173	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5697	2.12714319851843	No Hit
CCCATGTACTCTGCGTTGATACCAC	5312	1.9833920783798316	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3145	1.1742786307425772	No Hit
CATGTACTCTGCGTTGATACCACTG	3001	1.1205119780154131	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2793	1.0428490351872872	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2199	0.8210615926877352	No Hit
GCGTTGATACCACTGCTTCCCATGT	1944	0.7258498118167155	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1909	0.712781528167752	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1729	0.6455732122587968	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1645	0.6142093315012844	No Hit
TATCAACGCAGAGTACATGGGAAGC	1448	0.5406535635342613	No Hit
GGTATCAACGCAGAGTACATGGGAA	1439	0.5372931477388135	No Hit
GTGGTATCAACGCAGAGTACATGGG	1348	0.5033156102515085	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	1286	0.4801660792162017	No Hit
GTATCAACGCAGAGTACATGGGAAG	1284	0.4794193201505466	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1117	0.4170649381683494	No Hit
GTACTCTGCGTTGATACCACTGCTT	1016	0.37935360535276896	No Hit
GCTTCCCATGTACTCTGCGTTGATA	997	0.3722593942290459	No Hit
CAGTGGTATCAACGCAGAGTACATG	970	0.36217814684270266	No Hit
ATTTAGGGCTGCATTCCCAAACAAC	939	0.3506033813250493	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	884	0.3300675070195352	No Hit
ATACCACTGCTTCCCATGTACTCTG	881	0.3289473684210526	No Hit
GTACTTGTTCGCTATCGGTCTCTCG	880	0.3285739888882251	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	847	0.31625246430491666	No Hit
CATGGGAAGCAGTGGTATCAACGCA	819	0.3057978373857459	No Hit
GATACCACTGCTTCCCATGTACTCT	807	0.30131728299181554	No Hit
GTTGATACCACTGCTTCCCATGTAC	772	0.28824899934285203	No Hit
GTATTTAGCCTTAGATGGAATTTAC	766	0.28600872214588685	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	760	0.28376844494892167	No Hit
GCAGTGGTATCAACGCAGAGTACAT	759	0.28339506541609416	No Hit
CTGCTACTCCACGAGGCGCTGTCGC	689	0.25725849811816714	No Hit
TTACTAAGCGGAGGAAAAGAAACTA	669	0.24979090746161656	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	666	0.24867076886313397	No Hit
CCACTGCTTCCCATGTACTCTGCGT	656	0.2449369735348587	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	647	0.24157655773941095	No Hit
GAGTAGCAGGGTCCGGGGTCGACGG	640	0.23896290100961826	No Hit
TACCACTGCTTCCCATGTACTCTGC	627	0.2341089670828604	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	625	0.23336220801720536	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	617	0.2303751717545851	No Hit
GTCCGCTACAGAGGACGCTTCTTTA	600	0.22402771969651714	No Hit
CTTCCCATGTACTCTGCGTTGATAC	566	0.21133281558038114	No Hit
TTCCCAAACAACCCGACTCGGCGAC	560	0.209092538383416	No Hit
ACCCCGGACCCTGCTACTCCACGAG	553	0.2064788816536233	No Hit
ACATGGGAAGCAGTGGTATCAACGC	543	0.20274508632534802	No Hit
TCTTTAGACTACAATTCGGACAGCG	540	0.20162494772686543	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	515	0.1922904594061772	No Hit
CGCCAGTATTTAGCCTTAGATGGAA	494	0.1844494892167991	No Hit
GTCCGGGGTCGACGGGGCTCTCACC	481	0.17959555529004123	No Hit
GTAGCGGACCGGGCCCAAGTTCCCT	470	0.1754883804289384	No Hit
GCATATTACTAAGCGGAGGAAAAGA	468	0.17474162136328336	No Hit
ACCGGGAAGAGCCCAACTTGAAAAT	457	0.17063444650218054	No Hit
CTATCGGTCTCTCGCCAGTATTTAG	457	0.17063444650218054	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	456	0.170261066969353	No Hit
CCGCTACAGAGGACGCTTCTTTAGA	447	0.16690065117390526	No Hit
TGATACCACTGCTTCCCATGTACTC	440	0.16428699444411254	No Hit
GTCTAAAGAAGCGTCCTCTGTAGCG	430	0.16055319911583726	No Hit
CTACAGAGGACGCTTCTTTAGACTA	427	0.15943306051735467	No Hit
GCCCTAAATGGGTGGTAAATTCCAT	426	0.15905968098452714	No Hit
GGTGAGAGCCCCGTCGACCCCGGAC	419	0.15644602425473444	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	415	0.15495250612342434	No Hit
CGGTACTTGTTCGCTATCGGTCTCT	414	0.1545791265905968	No Hit
CCCCTAGTAACGGCGAGCGAACCGG	387	0.14449787920425355	No Hit
GTGGTATCAACGCAGAGTACTTTTT	387	0.14449787920425355	No Hit
CCTCAGATCAGACAAGATTACCCGC	378	0.14113746340880579	No Hit
TTCCCATGTACTCTGCGTTGATACC	370	0.13815042714618556	No Hit
GCCTTAGATGGAATTTACCACCCAT	363	0.13553677041639284	No Hit
GCTCTCACCCTCTCTGACGTCCCAT	362	0.13516339088356533	No Hit
CTGTAGCGGACCGGGCCCAAGTTCC	356	0.13292311368660015	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	349	0.13030945695680746	No Hit
GTACATGGGCATTGACCTCAGATCA	346	0.12918931835832487	No Hit
GGATTCCCCTAGTAACGGCGAGCGA	346	0.12918931835832487	No Hit
AGATTACCCGCCGAATTTAAGCATA	345	0.12881593882549733	No Hit
ACAGAGGACGCTTCTTTAGACTACA	342	0.12769580022701477	No Hit
CCATGTACTCTGCGTTGATACCACT	336	0.1254555230300496	No Hit
GTTTGGGAATGCAGCCCTAAATGGG	334	0.12470876396439452	No Hit
GGTATCAACGCAGAGTACATGGGGG	330	0.12321524583308442	No Hit
CTTCCCGGTTCGCTCGCCGTTACTA	324	0.12097496863611924	No Hit
CCTCTGTAGCGGACCGGGCCCAAGT	323	0.12060158910329172	No Hit
GGTATCAACGCAGAGTACATGGGCA	320	0.11948145050480913	No Hit
GATCAGACAAGATTACCCGCCGAAT	304	0.11350737797956867	No Hit
TAGTAACGGCGAGCGAACCGGGAAG	291	0.1086534440528108	No Hit
GAATTTACCACCCATTTAGGGCTGC	290	0.10828006451998326	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	285	0.10641316685584562	No Hit
CCCATTTAGGGCTGCATTCCCAAAC	282	0.10529302825736304	No Hit
GTCCTCTGTAGCGGACCGGGCCCAA	279	0.10417288965888045	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	277	0.1034261305932254	No Hit
ATCAACGCAGAGTACATGGGAAGCA	275	0.10267937152757035	No Hit
GTACATGGTAAGCAGTGGTATCAAC	272	0.10155923292908776	No Hit
CCGTTACTAGGGGAATCCTTGTTAG	271	0.10118585339626023	No Hit
CCCTGCTACTCCACGAGGCGCTGTC	268	0.10006571479777764	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.7337953282752854E-4	0.0
11	0.0	0.0	0.0	3.7337953282752854E-4	0.0
12	0.0	0.0	0.0	7.467590656550571E-4	0.0
13	0.0	0.0	0.0	7.467590656550571E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCAAA	25	0.006005931	19.007307	1
TAACAAC	40	2.7423282E-4	16.631392	1
GACCAAA	35	0.0021583228	16.291977	1
GAACAAA	285	0.0	14.672306	1
GAGAACA	65	5.4000444E-5	13.158905	1
GCATATT	110	3.7407517E-8	12.095559	1
CGGACCC	130	2.568413E-9	11.692424	5
AGTAGCA	155	2.5465852E-11	11.649639	2
CCGGACC	135	4.614776E-9	11.263589	4
ACCGATA	95	1.2879147E-5	11.045614	19
GGAAACA	70	0.0014796503	10.861318	1
GAGTAGC	160	5.275069E-10	10.691609	1
CATATTA	125	2.1975029E-7	10.644091	2
AGAAACT	125	2.226061E-7	10.6341305	18
GAAAACA	155	3.5652192E-9	10.423362	1
TAAGCAT	110	6.0232323E-6	10.35792	18
ATATTAC	130	3.768564E-7	10.234704	3
AGAACAA	75	0.0026269131	10.13723	2
GTACTTA	75	0.0026269131	10.13723	1
GGACCCT	150	2.4288966E-8	10.125849	6
>>END_MODULE