FastQCFastQC Report
Wed 25 May 2016
SRR1294838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267824
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84933.1711123723041994No Hit
GGTATCAACGCAGAGTACTTTTTTT69992.6132833502598722No Hit
GTACATGGGAAGCAGTGGTATCAAC57142.133490650576498No Hit
CCCATGTACTCTGCGTTGATACCAC56602.1133281558038117No Hit
TATCAACGCAGAGTACTTTTTTTTT54922.0506003942887867No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42511.587236394049824No Hit
GAGTACATGGGAAGCAGTGGTATCA32661.2194575542147081No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31731.184733257661748No Hit
CATGTACTCTGCGTTGATACCACTG31071.1600902084951312No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27001.008124738634327No Hit
ACGCAGAGTACATGGGAAGCAGTGG20620.7699085966903638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA19620.7325706434076109No Hit
GCGTTGATACCACTGCTTCCCATGT19530.7292102276121633No Hit
GCAGAGTACTTTTTTTTTTTTTTTT17690.6605083935718981No Hit
ACTCTGCGTTGATACCACTGCTTCC16820.628024374215903No Hit
GGTATCAACGCAGAGTACATGGGAA15340.5727642033574287No Hit
TATCAACGCAGAGTACATGGGAAGC14440.5391600454029511No Hit
GTGGTATCAACGCAGAGTACATGGG14090.5260917617539878No Hit
GTATCAACGCAGAGTACATGGGAAG12880.48091283828185677No Hit
GTGGTATCAACGCAGAGTACTTTTT11650.4349871557440707No Hit
GCTTCCCATGTACTCTGCGTTGATA10310.3849542983451819No Hit
CAGTGGTATCAACGCAGAGTACATG10130.3782334667542864No Hit
GTACTCTGCGTTGATACCACTGCTT9820.366658701236633No Hit
GTACTTGTTCGCTATCGGTCTCTCG9110.3401487544058785No Hit
ATTTAGGGCTGCATTCCCAAACAAC9090.3394019953402234No Hit
GCAGAGTACATGGGAAGCAGTGGTA8960.33454806141346555No Hit
GATACCACTGCTTCCCATGTACTCT8920.33305454328215545No Hit
GGGAAGCAGTGGTATCAACGCAGAG8560.3196128801003644No Hit
ATACCACTGCTTCCCATGTACTCTG8520.31811936196905427No Hit
ATCAACGCAGAGTACTTTTTTTTTT8260.3084114941155386No Hit
CATGGGAAGCAGTGGTATCAACGCA8200.3061712169185734No Hit
CTGCTTCCCATGTACTCTGCGTTGA8150.3043043192544358No Hit
GTTGATACCACTGCTTCCCATGTAC7890.29459645140092No Hit
GCAGTGGTATCAACGCAGAGTACAT7700.28750224027719695No Hit
AAGCAGTGGTATCAACGCAGAGTAC7080.2643527092418902No Hit
TTACTAAGCGGAGGAAAAGAAACTA7050.2632325706434076No Hit
GTATTTAGCCTTAGATGGAATTTAC7020.262112432044925No Hit
CTGCTACTCCACGAGGCGCTGTCGC6930.25875201624947725No Hit
CCACTGCTTCCCATGTACTCTGCGT6640.24792400979747894No Hit
TACCACTGCTTCCCATGTACTCTGC6360.23746938287830816No Hit
GAGTAGCAGGGTCCGGGGTCGACGG6330.23634924427982557No Hit
ACATGGGAAGCAGTGGTATCAACGC5830.2176802676384491No Hit
ATGGGAAGCAGTGGTATCAACGCAG5650.21095943604755363No Hit
ACCCCGGACCCTGCTACTCCACGAG5560.20759902025210586No Hit
CTTCCCATGTACTCTGCGTTGATAC5560.20759902025210586No Hit
GTCCGCTACAGAGGACGCTTCTTTA5400.20162494772686543No Hit
GTAGCGGACCGGGCCCAAGTTCCCT5320.19863791146424517No Hit
TCTTTAGACTACAATTCGGACAGCG5310.19826453193141766No Hit
ACCGGGAAGAGCCCAACTTGAAAAT5170.19303721847183225No Hit
TTCCCAAACAACCCGACTCGGCGAC5130.19154370034052212No Hit
GCATATTACTAAGCGGAGGAAAAGA5070.18930342314355694No Hit
CGCCAGTATTTAGCCTTAGATGGAA5050.1885566640779019No Hit
CTACAGAGGACGCTTCTTTAGACTA4930.18407610968397156No Hit
TGATACCACTGCTTCCCATGTACTC4710.17586175996176595No Hit
CTATCGGTCTCTCGCCAGTATTTAG4680.17474162136328336No Hit
CCCCTAGTAACGGCGAGCGAACCGG4490.1676474102395603No Hit
GCCCTAAATGGGTGGTAAATTCCAT4430.16540713304259513No Hit
GGTGAGAGCCCCGTCGACCCCGGAC4400.16428699444411254No Hit
GTCCGGGGTCGACGGGGCTCTCACC4250.15868630145169962No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4200.15681940378756198No Hit
CGGTACTTGTTCGCTATCGGTCTCT4130.1542057470577693No Hit
CCGCTACAGAGGACGCTTCTTTAGA4130.1542057470577693No Hit
CAACGCAGAGTACTTTTTTTTTTTT4020.15009857219666647No Hit
AACGCAGAGTACTTTTTTTTTTTTT4000.14935181313101142No Hit
GTTTGGGAATGCAGCCCTAAATGGG4000.14935181313101142No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3930.1467381564012187No Hit
GTCTAAAGAAGCGTCCTCTGTAGCG3910.14599139733556366No Hit
GCCTTAGATGGAATTTACCACCCAT3800.14188422247446084No Hit
TTCCCATGTACTCTGCGTTGATACC3730.13927056574466815No Hit
CTGTAGCGGACCGGGCCCAAGTTCC3700.13815042714618556No Hit
GCTCTCACCCTCTCTGACGTCCCAT3650.13628352948204792No Hit
CCATGTACTCTGCGTTGATACCACT3600.13441663181791028No Hit
GTACATGGGCATTGACCTCAGATCA3580.1336698727522552No Hit
CCTCAGATCAGACAAGATTACCCGC3520.13142959555529002No Hit
ACAGAGGACGCTTCTTTAGACTACA3510.1310562160224625No Hit
AGATTACCCGCCGAATTTAAGCATA3410.12732242069418723No Hit
CCTCTGTAGCGGACCGGGCCCAAGT3390.12657566162853218No Hit
CTTCCCGGTTCGCTCGCCGTTACTA3360.1254555230300496No Hit
TAGTAACGGCGAGCGAACCGGGAAG3320.12396200489873947No Hit
TCAACGCAGAGTACTTTTTTTTTTT3280.12246848676742934No Hit
GGATTCCCCTAGTAACGGCGAGCGA3280.12246848676742934No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3190.1191080709719816No Hit
GATCAGACAAGATTACCCGCCGAAT3150.11761455284067149No Hit
CCGTTACTAGGGGAATCCTTGTTAG3130.11686779377501644No Hit
CCCATTTAGGGCTGCATTCCCAAAC3110.11612103470936139No Hit
GAATTTACCACCCATTTAGGGCTGC3090.11537427564370631No Hit
GGTATCAACGCAGAGTACATGGGCA3040.11350737797956867No Hit
GGTATCAACGCAGAGTACATGGGGG3020.11276061891391362No Hit
GGAAGCAGTGGTATCAACGCAGAGT2870.10715992592150068No Hit
CCCTGCTACTCCACGAGGCGCTGTC2860.10678654638867316No Hit
ATCAACGCAGAGTACATGGGAAGCA2820.10529302825736304No Hit
CTTCTTTAGACTACAATTCGGACAG2690.10043909433060517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTC250.005889381719.0718843
TGTCCCG250.006070490518.97218111
GAACAAA1200.015.8932371
TGCATTC2053.6379788E-1210.18019510
TAGTAAT750.002553987510.1716724
TATGCTT750.002667582410.11849710
CGGACCC1053.9041865E-59.9900355
CTGCATT2105.456968E-129.937819
GCTGCAT2151.0913936E-119.7066978
GGCTGCA2251.8189894E-129.6987037
CCGGACC1106.503288E-59.5359424
GAAACTA1502.6342423E-79.4931819
CATCCTG800.00454953879.48609118
GCATTCC2151.05501385E-109.26548411
ACACAAA1553.9705992E-79.2283311
TAGGGCT2303.8198777E-119.1213364
GGACCCT1151.1098076E-49.0804336
AGCCCTA1151.11888345E-49.07365113
ATTCCCA2304.1836756E-119.07365113
GGCTCTC1151.11888345E-49.07365116
Produced by FastQC (version 0.11.5)