FastQCFastQC Report
Wed 25 May 2016
SRR1294837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348051
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC244747.03172810881164No Hit
CCCATGTACTCTGCGTTGATACCAC234286.731197439455712No Hit
GAGTACATGGGAAGCAGTGGTATCA124743.5839575234663883No Hit
CATGTACTCTGCGTTGATACCACTG100852.897563862767238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA92472.656794550223961No Hit
GCGTTGATACCACTGCTTCCCATGT83152.3890177014288136No Hit
ACGCAGAGTACATGGGAAGCAGTGG81852.3516668534209066No Hit
GGTATCAACGCAGAGTACATGGGAA74662.145087932515637No Hit
ACTCTGCGTTGATACCACTGCTTCC69311.991374827252328No Hit
GTATCAACGCAGAGTACATGGGAAG69121.9859158571588647No Hit
TATCAACGCAGAGTACATGGGAAGC67811.9482776949355123No Hit
GTGGTATCAACGCAGAGTACATGGG51161.4698995262188588No Hit
GTACTCTGCGTTGATACCACTGCTT47761.3722126929674099No Hit
GCTTCCCATGTACTCTGCGTTGATA46981.3498021841626657No Hit
GATACCACTGCTTCCCATGTACTCT42501.2210854156431097No Hit
ATACCACTGCTTCCCATGTACTCTG38511.1064470436803802No Hit
CAGTGGTATCAACGCAGAGTACATG38151.0961037319243445No Hit
GGGAAGCAGTGGTATCAACGCAGAG36021.0349058040344663No Hit
CTGCTTCCCATGTACTCTGCGTTGA34520.9918086717176505No Hit
CATGGGAAGCAGTGGTATCAACGCA33000.948136910969944No Hit
GCAGAGTACATGGGAAGCAGTGGTA31860.9153830904091642No Hit
GTTGATACCACTGCTTCCCATGTAC31540.9061890355149103No Hit
GCAGTGGTATCAACGCAGAGTACAT30410.8737225291695757No Hit
TACCACTGCTTCCCATGTACTCTGC29560.8493008208567135No Hit
ACATGGGAAGCAGTGGTATCAACGC28330.8139611723569248No Hit
CCACTGCTTCCCATGTACTCTGCGT27910.8018939753082164No Hit
CTTCCCATGTACTCTGCGTTGATAC26320.7562110150523917No Hit
AAGCAGTGGTATCAACGCAGAGTAC23740.6820839474674689No Hit
ATGGGAAGCAGTGGTATCAACGCAG22310.6409980146587713No Hit
CCATGTACTCTGCGTTGATACCACT21130.6070949372362096No Hit
TGATACCACTGCTTCCCATGTACTC20920.6010613387118555No Hit
TTCCCATGTACTCTGCGTTGATACC18690.5369902686675229No Hit
ATCAACGCAGAGTACATGGGAAGCA17150.49274387948892545No Hit
GTATCAACGCAGAGTACTTTTTTTT16840.4838371388101169No Hit
AAACAAAAAAAAAAAAAAAAAAAAA13520.3884488192822316No Hit
GTACATGGTAAGCAGTGGTATCAAC13440.38615030555866814No Hit
GGTATCAACGCAGAGTACTTTTTTT13090.3760943080180778No Hit
GGAAGCAGTGGTATCAACGCAGAGT12650.36345248253847856No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10770.3094374100347363No Hit
TATCAACGCAGAGTACTTTTTTTTT10770.3094374100347363No Hit
ACCATGTACTCTGCGTTGATACCAC10560.3034038115103821No Hit
CTGCGTTGATACCACTGCTTCCCAT10260.294784385047019No Hit
CTCTGCGTTGATACCACTGCTTCCC9900.2844410732909832No Hit
TCAACGCAGAGTACATGGGAAGCAG9320.26777684879514785No Hit
AAAAAGTACTCTGCGTTGATACCAC8890.255422337530994No Hit
TGGGAAGCAGTGGTATCAACGCAGA8690.24967605322208528No Hit
TCCATGTACTCTGCGTTGATACCAC7850.2255416591246685No Hit
AGTGGTATCAACGCAGAGTACATGG7590.21807148952308714No Hit
AACGCAGAGTACATGGGAAGCAGTG7400.21261251942962384No Hit
GGTAAGCAGTGGTATCAACGCAGAG7390.2123252052141784No Hit
AAAGTACTCTGCGTTGATACCACTG7050.2025565218890335No Hit
ATGTACTCTGCGTTGATACCACTGC6790.1950863522874521No Hit
TCCCATGTACTCTGCGTTGATACCA6760.1942244096411158No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6590.18934006797854339No Hit
ACTGCTTCCCATGTACTCTGCGTTG6570.18876543954765249No Hit
GTACATGGGTAAGCAGTGGTATCAA6470.18589229739319812No Hit
CCCCATGTACTCTGCGTTGATACCA6420.18445572631597096No Hit
GAACAAAAAAAAAAAAAAAAAAAAA6300.1810079557306257No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6230.1789967562225076No Hit
AAAAACAAAAAAAAAAAAAAAAAAA5960.1712392724054808No Hit
TTGATACCACTGCTTCCCATGTACT5880.1689407586819173No Hit
GAGTACATGGTAAGCAGTGGTATCA5730.16463104545023574No Hit
CAACGCAGAGTACATGGGAAGCAGT5390.15486236212509086No Hit
GTACATGGAAAGCAGTGGTATCAAC5030.1445190503690551No Hit
GCTTACCATGTACTCTGCGTTGATA4810.13819813762925548No Hit
CGCAGAGTACATGGGAAGCAGTGGT4650.13360111018212847No Hit
CGTTGATACCACTGCTTCCCATGTA4600.1321645391049013No Hit
ACTCTGCGTTGATACCACTGCTTAC4490.12900408273500147No Hit
GAGTACATGGAAAGCAGTGGTATCA4130.11866077097896573No Hit
ACGCAGAGTACATGGTAAGCAGTGG4100.1177988283326294No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3990.11463837196272961No Hit
ACACAAAAAAAAAAAAAAAAAAAAA3800.10917940186926628No Hit
TGTACTCTGCGTTGATACCACTGCT3780.10860477343837541No Hit
GCGTTGATACCACTGCTTACCATGT3760.10803014500748453No Hit
GTACATGGAAGCAGTGGTATCAACG3590.1031458033449121No Hit
CACTGCTTCCCATGTACTCTGCGTT3580.10285848912946667No Hit
CTGCTTACCATGTACTCTGCGTTGA3520.10113460383679403No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA3510.10084728962134859No Hit
GCTTTCCATGTACTCTGCGTTGATA3510.10084728962134859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA555.8766454E-717.272861
GAACAAA3050.016.5083221
AGAACAA400.005267916214.2501082
TACAAAA1152.5465852E-1114.0435852
GGAAACA604.0775558E-412.6667621
CGCAAAA701.0861243E-412.2143772
AAAAGTA1803.45608E-1110.5556352
AAAAAGT1803.45608E-1110.5556351
GAAAAAA8750.09.12006951
AAGTACT2106.530172E-109.048994
AAAGTAC2106.548362E-109.0476883
GAAAACA1856.477785E-88.7297951
GAAACAA1201.7526501E-48.70841
ACGCAAA1000.0028925468.5500651
AGTACTC2159.515134E-98.3966225
TAAAAAA2053.79805E-88.3415271
ATGGGTA1907.743709E-67.50113735
GCAAAAA1658.4127634E-57.48490523
GGGTAAG1906.2822015E-56.9990457
CCATGTA72500.06.9841912
Produced by FastQC (version 0.11.5)