##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294837_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348051
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.34018577737171	33.0	31.0	34.0	19.0	34.0
2	30.554082591344372	33.0	31.0	34.0	23.0	34.0
3	30.89910099381987	34.0	31.0	34.0	25.0	34.0
4	33.648456691691734	37.0	35.0	37.0	24.0	37.0
5	34.524971915035444	37.0	35.0	37.0	31.0	37.0
6	34.19106108012906	37.0	35.0	37.0	27.0	37.0
7	34.488324986855375	37.0	35.0	37.0	30.0	37.0
8	34.323380768910305	37.0	35.0	37.0	28.0	37.0
9	35.75433198008338	39.0	37.0	39.0	27.0	39.0
10	36.02153994673194	39.0	38.0	39.0	30.0	39.0
11	35.945792426971906	39.0	38.0	39.0	28.0	39.0
12	36.091854354677906	39.0	38.0	39.0	29.0	39.0
13	35.705123674404035	39.0	37.0	39.0	27.0	39.0
14	37.23939595059345	41.0	38.0	41.0	27.0	41.0
15	37.073696096261756	41.0	38.0	41.0	27.0	41.0
16	37.046536283475696	40.0	38.0	41.0	27.0	41.0
17	37.14818517975814	41.0	38.0	41.0	28.0	41.0
18	37.08477205926717	41.0	38.0	41.0	28.0	41.0
19	37.01764683911266	40.0	38.0	41.0	28.0	41.0
20	37.051771148481116	41.0	38.0	41.0	27.0	41.0
21	37.00420053382981	41.0	38.0	41.0	27.0	41.0
22	37.08914785476841	41.0	38.0	41.0	27.0	41.0
23	37.12940919577878	41.0	38.0	41.0	27.0	41.0
24	37.28523406052561	41.0	38.0	41.0	28.0	41.0
25	36.41497079451	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.10901356741270618
1101	2	-0.11263093232831523
1101	3	-0.2637095849544764
1101	4	-0.2187735541259741
1101	5	-0.4463073796643613
1101	6	-0.23451886116757947
1101	7	-0.10137679521800891
1101	8	-0.1552229706516357
1101	9	-0.19530697623821425
1101	10	-0.07148743769990062
1101	11	-0.04933407745342322
1101	12	0.14030035117931305
1101	13	0.20385917563345402
1101	14	0.24705155782786647
1101	15	-0.21877872220885308
1101	16	-0.1276817230596876
1101	17	-0.26065722044626227
1101	18	-0.09053821033564446
1101	19	-0.2018504454669383
1101	20	-0.14521111866972092
1101	21	-0.03253538629338948
1101	22	-0.0640495051694785
1101	23	0.09319563507319373
1101	24	0.016189466701440836
1101	25	0.024808949012559367
1102	1	-0.5294700254865603
1102	2	-0.2712130846829801
1102	3	-0.47597016326935204
1102	4	-0.34864614307807074
1102	5	-0.5406369989460131
1102	6	-0.6359562328308215
1102	7	-0.2004696016272689
1102	8	-0.3952716123859048
1102	9	-0.2414661306462662
1102	10	-0.5057116946090474
1102	11	-0.47117909714575035
1102	12	-0.4789223909353453
1102	13	-0.19136753860897926
1102	14	-0.4279913613494344
1102	15	-0.7900750496737601
1102	16	-0.48387723253556914
1102	17	-0.8279435502675128
1102	18	-0.393640172836065
1102	19	-0.48821392882102543
1102	20	-0.7432267378840436
1102	21	-0.7290673484571926
1102	22	-0.7791082455336991
1102	23	-0.8496713659703303
1102	24	-0.7219614741542486
1102	25	-0.633773203342102
1103	1	-0.2582345354616642
1103	2	-0.4316877508322996
1103	3	-0.5805996132644999
1103	4	-0.49995001994941646
1103	5	-0.35095646090481836
1103	6	-0.3825045563022229
1103	7	-0.1667531079486082
1103	8	-0.2871258174494429
1103	9	-0.054307660700914084
1103	10	-0.06957175216393097
1103	11	-0.23909224746747526
1103	12	0.014829023686182552
1103	13	-0.130288222800786
1103	14	-0.21728550562465898
1103	15	-0.4216429353400031
1103	16	-0.6335361066585037
1103	17	-0.6466820312065025
1103	18	-0.13644581192165361
1103	19	-0.536973912007241
1103	20	-0.4995157410204669
1103	21	-0.41942286305608434
1103	22	-0.6064031658298816
1103	23	-0.7477943652913126
1103	24	-0.5525544834988381
1103	25	-0.6670289345801805
1104	1	-0.13904544218180703
1104	2	0.009738969641734485
1104	3	-0.09164167709059967
1104	4	0.014281698337661908
1104	5	-0.5021741414112029
1104	6	-0.47905246714044836
1104	7	-0.09046548611866001
1104	8	-0.20900197798025744
1104	9	-0.2272504779951916
1104	10	-0.6214603742166958
1104	11	-0.15865766220508704
1104	12	-0.2747074242023686
1104	13	-0.3446016424569791
1104	14	-0.3579934191037353
1104	15	0.1329649393515524
1104	16	-0.05360080754071106
1104	17	-0.7428296878626881
1104	18	-0.2427896343903555
1104	19	-0.453506472891263
1104	20	-0.06051530362929469
1104	21	-0.032183131432702794
1104	22	-0.36627128826639677
1104	23	-0.18698874027583656
1104	24	-0.18054916878350724
1104	25	-0.5515659188918676
1105	1	-0.39195778568113226
1105	2	-0.4197964259129634
1105	3	-0.4287600492417454
1105	4	-0.24399580668084297
1105	5	-0.3654814349673643
1105	6	-0.3421219361063521
1105	7	-0.3661807738058016
1105	8	-0.46273663023233524
1105	9	-0.6130745200458065
1105	10	-0.3968169095558238
1105	11	-0.5301275794098785
1105	12	-0.45850955414327643
1105	13	-0.8055056983739775
1105	14	-0.5862244072447567
1105	15	-0.34664664069052975
1105	16	-0.5541731989274297
1105	17	-0.3237464815034983
1105	18	-0.5187467775930017
1105	19	-0.2439547923985259
1105	20	-0.4508217345674552
1105	21	-0.5701907256958876
1105	22	-0.6080905254541022
1105	23	-0.6174230321631953
1105	24	-0.2284446227060073
1105	25	-0.4557669491963594
1106	1	0.2168063211176552
1106	2	0.2592787185957093
1106	3	-0.03163370737551929
1106	4	0.21102208486884422
1106	5	0.23131460136624327
1106	6	0.07547233859774849
1106	7	0.0537146294422044
1106	8	0.07246843445250306
1106	9	0.1723590059657525
1106	10	0.07469423825590837
1106	11	-0.016370353976356
1106	12	-0.06706446743807248
1106	13	0.20179405389687588
1106	14	0.08324985644916438
1106	15	0.1981372844402145
1106	16	0.05081441461509684
1106	17	0.18932811249595005
1106	18	0.19189456564949126
1106	19	0.018483963450634633
1106	20	-0.054928533356331855
1106	21	0.05605755396279477
1106	22	0.29204710185115346
1106	23	0.3379086496424719
1106	24	-0.009088718494609793
1106	25	-0.10613854292055436
1107	1	-0.12919421898496353
1107	2	-0.39108044964625677
1107	3	-0.11719316367221566
1107	4	-0.5228729551887668
1107	5	-0.3033581864634485
1107	6	-0.1283947961578562
1107	7	-0.1977967577213562
1107	8	-0.11937601599308323
1107	9	-0.30698566952258943
1107	10	-0.16952216286030364
1107	11	0.016572305128875087
1107	12	-0.1122314491646037
1107	13	-0.3076091327085777
1107	14	-0.1474279723383063
1107	15	-0.3421993795259368
1107	16	-0.3423946951157504
1107	17	0.15692195532609077
1107	18	-0.36157379333871376
1107	19	0.04331788189514185
1107	20	-0.03543442947659514
1107	21	-0.05544483149044055
1107	22	-0.26791329062341873
1107	23	-0.11852847341090467
1107	24	-0.19012751586610932
1107	25	0.1924841953997216
1108	1	0.033602539605048776
1108	2	0.3209698950662947
1108	3	0.37003996209227097
1108	4	-0.059832256867849765
1108	5	0.2524560579488764
1108	6	-0.01647093871317651
1108	7	-0.05976926411521788
1108	8	0.1532457453768714
1108	9	-0.06656256266169436
1108	10	0.0877369553427414
1108	11	0.06272278327854508
1108	12	0.07541102275800426
1108	13	0.05748733120780258
1108	14	0.12757758754160164
1108	15	0.040084063151702765
1108	16	0.006630240945632693
1108	17	-0.18278323204186364
1108	18	0.045336442400191856
1108	19	0.22004908950105317
1108	20	0.041549057680079216
1108	21	-0.022853510462979898
1108	22	-0.016391273498982173
1108	23	0.018216772891776145
1108	24	-0.10771967087556078
1108	25	0.20105683523070184
1109	1	-0.09579441148307666
1109	2	0.18975490907189752
1109	3	0.03774358200177019
1109	4	-0.1075859737392193
1109	5	0.10142449147613064
1109	6	0.06001446313987202
1109	7	0.07063770636528233
1109	8	-0.0993264373423699
1109	9	0.10869600230275012
1109	10	0.20473086829253617
1109	11	0.27344649584049563
1109	12	-0.25468351505712405
1109	13	0.009046801149622752
1109	14	0.2198798930791952
1109	15	0.16626915257208452
1109	16	0.41704152084220425
1109	17	0.18932811249595005
1109	18	-0.3541127603578289
1109	19	-0.11572849076181768
1109	20	0.015840697412897953
1109	21	0.04887806678330264
1109	22	0.3517540615581112
1109	23	0.349557001290826
1109	24	0.21428124487535172
1109	25	0.24111420433219166
1110	1	0.19550761981895448
1110	2	0.2520059913229815
1110	3	0.14949183375002306
1110	4	0.15976667361343289
1110	5	0.25356568361732457
1110	6	0.027680130805542547
1110	7	0.20133367706124972
1110	8	-0.07714195515789157
1110	9	0.27720749081424145
1110	10	-0.1711066275449582
1110	11	0.2502963126903097
1110	12	0.04869310831949747
1110	13	-0.15405010194727708
1110	14	-0.0993475461482376
1110	15	0.15043165673458958
1110	16	0.19072783452851638
1110	17	0.12906837223621181
1110	18	0.4538859076408386
1110	19	0.37458785955453067
1110	20	0.6104394320116313
1110	21	0.7071398050450455
1110	22	0.7509215607256152
1110	23	0.5959173076511348
1110	24	0.642166692760803
1110	25	0.6312640544820383
1111	1	-0.20740658372793774
1111	2	-0.09826082446229023
1111	3	-0.16321626658549349
1111	4	-0.07638606644088952
1111	5	-0.06791378594650155
1111	6	-0.5190845280856564
1111	7	-0.022813950355264012
1111	8	-0.21108667476165976
1111	9	-0.3659655400213566
1111	10	-0.6530654069026838
1111	11	-0.6671729315979107
1111	12	-0.28292855648318493
1111	13	-0.37503748012270677
1111	14	-0.5394466035056453
1111	15	-0.6265007605844701
1111	16	-0.14616943340566024
1111	17	-0.4285478603888109
1111	18	-0.7547352778520562
1111	19	-0.46919868781725427
1111	20	-0.8520529789166318
1111	21	-0.6835424125616001
1111	22	-0.6752807080074135
1111	23	-0.6691346175765389
1111	24	-0.5828404968861065
1111	25	-0.5899949325765945
1112	1	-0.5014156898165041
1112	2	-0.1346647090249249
1112	3	-0.39429276767184973
1112	4	-0.7796252489398867
1112	5	-0.29779719643108393
1112	6	-0.43545706477758017
1112	7	-0.5169025958172853
1112	8	-0.7731697875584302
1112	9	-0.6893603506620849
1112	10	-0.8215104998789471
1112	11	-0.6401529385576055
1112	12	-0.5545408956133357
1112	13	-0.7416525826612386
1112	14	-0.8610810206604143
1112	15	-0.6037326149338469
1112	16	-0.4768806967073047
1112	17	-0.5106956574113468
1112	18	-0.5334140469802477
1112	19	-0.9056329844932662
1112	20	-0.6490462736304821
1112	21	-0.8486187791328419
1112	22	-0.5817663836095832
1112	23	-0.586632987311809
1112	24	-0.5359122665494382
1112	25	-0.5945625980667444
1113	1	0.09350886514896573
1113	2	0.21160748571899646
1113	3	0.060662443961728485
1113	4	-0.03495312713376819
1113	5	0.33419457527361374
1113	6	-0.14280554789931443
1113	7	0.06530627980097847
1113	8	-0.2510149314966199
1113	9	-0.09083734106620511
1113	10	0.01337656049987146
1113	11	-0.20936122155626435
1113	12	-0.06779506104538058
1113	13	-0.10855819659236232
1113	14	0.007613848621375041
1113	15	-0.19289337439866472
1113	16	0.20473157051855395
1113	17	-0.022756036232031818
1113	18	0.14638902096587003
1113	19	-0.037983746921185
1113	20	-0.023066171973425753
1113	21	0.059867077772317145
1113	22	0.02324410119884135
1113	23	0.15046898884664728
1113	24	0.3961330688504674
1113	25	0.28226654514206473
1114	1	0.24863893295026784
1114	2	0.5430497623667527
1114	3	0.3601989044570928
1114	4	0.49262862647538697
1114	5	0.6454175354691785
1114	6	0.6519225550479675
1114	7	0.24840438413195898
1114	8	0.23470989669396403
1114	9	0.5584869520937019
1114	10	0.542226705788373
1114	11	0.39332661572061767
1114	12	0.05219479182118647
1114	13	0.405613197716022
1114	14	0.4591036323029343
1114	15	0.4835629698659005
1114	16	0.4024450062456921
1114	17	0.5349269580947933
1114	18	0.31556940932433974
1114	19	0.6369447619114368
1114	20	0.7117188932910921
1114	21	0.5076785255837635
1114	22	1.1135478233518725
1114	23	0.9387455904794137
1114	24	0.8810219116160241
1114	25	0.6384021016200876
1115	1	-0.057894139711837767
1115	2	0.19766581536990202
1115	3	-0.01461373809748423
1115	4	0.14049981144334112
1115	5	0.3998706689545699
1115	6	0.09630183168530948
1115	7	-0.008666322938744031
1115	8	-0.08121820149219872
1115	9	-0.20484529510951432
1115	10	0.03997841644331146
1115	11	-0.04357465505162139
1115	12	-0.008139736255280638
1115	13	0.1687064962931899
1115	14	0.13901022511275585
1115	15	-0.030741363793268306
1115	16	0.016651588194207534
1115	17	0.05038802033005396
1115	18	0.21667183139449975
1115	19	0.06244709709118723
1115	20	0.15058605957761273
1115	21	0.21470578744973778
1115	22	0.3598658422505423
1115	23	0.0660346097039195
1115	24	0.07917549041476235
1115	25	-0.005216883934380689
1116	1	0.47091901693247706
1116	2	0.13266660730274538
1116	3	0.4149939911670728
1116	4	0.29538339731526264
1116	5	0.23769602136468393
1116	6	0.28630235964053696
1116	7	0.14198834154570505
1116	8	0.25423135238563077
1116	9	0.29211896232145307
1116	10	0.3053075965288343
1116	11	0.3505254452598692
1116	12	0.1003550633857131
1116	13	0.1744664878459048
1116	14	0.35147524807880615
1116	15	0.28321249291968087
1116	16	0.2829522059018217
1116	17	0.5140884585328962
1116	18	0.4065060534524747
1116	19	0.3604619854286568
1116	20	0.33085901243121185
1116	21	0.45266576844334594
1116	22	0.6403130203298417
1116	23	0.54038390488369
1116	24	0.634864208011507
1116	25	0.18009012203151542
1201	1	-0.3744097355529483
1201	2	-0.18873781032991133
1201	3	-0.36133461253096755
1201	4	-0.5284151445683847
1201	5	-0.4419715064653218
1201	6	-0.3219066460539608
1201	7	-0.5743688022775899
1201	8	-0.35061302953805296
1201	9	-0.3366768067064214
1201	10	-0.2394537349829804
1201	11	-0.40557145590472743
1201	12	-0.3978358173003329
1201	13	-0.20809968872413975
1201	14	-0.26066986019782945
1201	15	-0.5361799135133438
1201	16	-0.5853878679508213
1201	17	-0.7678737765241195
1201	18	-0.4340479766566858
1201	19	-0.430701396643812
1201	20	-0.49129610517844924
1201	21	-0.44839738503760174
1201	22	-0.2999048855553781
1201	23	-0.16846285763812574
1201	24	-0.434940745255723
1201	25	-0.3302235488237457
1202	1	0.07697236749612557
1202	2	0.09246509134120018
1202	3	0.03489172444049515
1202	4	0.28516656430390697
1202	5	0.0418804950583862
1202	6	-0.03775984004524702
1202	7	0.04834770511736508
1202	8	0.03370519368525038
1202	9	-0.003539457628100706
1202	10	0.01505114254066342
1202	11	0.0341840882414175
1202	12	0.0818466882065465
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2210	5	0.31479933451349495
2210	6	0.2992125060679385
2210	7	0.07064095947688287
2210	8	0.23745194117667978
2210	9	0.506379136220346
2210	10	0.40718176257291105
2210	11	0.35317375496384784
2210	12	0.21004624896216484
2210	13	0.3225654317197666
2210	14	0.2630519371393447
2210	15	0.5569971338862132
2210	16	0.15903398663573398
2210	17	0.3973971305116848
2210	18	0.6120451196543968
2210	19	0.5358957878606816
2210	20	0.5222786052717652
2210	21	0.738375070667523
2210	22	0.6302692112988382
2210	23	0.6597119144652765
2210	24	0.4788145209539252
2210	25	0.723752347716335
2211	1	0.5681298137371193
2211	2	0.5938496467918561
2211	3	0.6732683024651962
2211	4	0.4822121440799165
2211	5	0.517335783919826
2211	6	0.7642188985176901
2211	7	0.3819641499158948
2211	8	0.48913093132515684
2211	9	0.5679923976762069
2211	10	0.626024402545788
2211	11	0.44581295016842404
2211	12	0.5848211134142289
2211	13	0.5819091402116143
2211	14	0.7280334441399319
2211	15	0.8467570826221831
2211	16	0.7529376737260947
2211	17	1.0332110246800923
2211	18	0.8240753679178638
2211	19	0.7322793099605178
2211	20	0.7972472651592213
2211	21	0.6658215436217034
2211	22	0.7841328328160415
2211	23	0.856761122330326
2211	24	0.5130662310508853
2211	25	0.657734361757953
2212	1	-0.04437834787189132
2212	2	0.07767593113926097
2212	3	-0.10993800470420467
2212	4	0.2518350929989239
2212	5	-0.17254138005071695
2212	6	0.01212739086255965
2212	7	-0.10849210691087308
2212	8	-0.008019370425550676
2212	9	-0.13560847370904128
2212	10	-0.16978892086140007
2212	11	-0.009006823198191682
2212	12	-0.14841173805363894
2212	13	0.04228185877492763
2212	14	-0.04671530034526228
2212	15	0.10708037387110636
2212	16	-1.134017176557954
2212	17	-0.48140359482112416
2212	18	-0.2116129837117171
2212	19	-0.056986419824625045
2212	20	0.12421200090615514
2212	21	-0.1805510406945814
2212	22	-0.0858855346180647
2212	23	-0.17921097869666625
2212	24	-0.17707942697312262
2212	25	0.09316459296794477
2213	1	0.051772307512212734
2213	2	-0.06874849228864477
2213	3	0.23476085044761064
2213	4	0.25884521412054085
2213	5	0.13478398912134537
2213	6	-0.06792902194646899
2213	7	-0.16274795559181143
2213	8	-0.05916421860872134
2213	9	-0.40464779675533435
2213	10	-0.16170031956722397
2213	11	-0.07807103424845963
2213	12	-0.20026174634963922
2213	13	-0.5630358780759153
2213	14	-0.3286549054556005
2213	15	-0.018189246172028106
2213	16	-0.3014304833440846
2213	17	-0.17121610519112096
2213	18	0.06203062007126192
2213	19	-0.09178814539290414
2213	20	-0.2642482612474879
2213	21	-0.26156149365625225
2213	22	-0.060333850529801225
2213	23	0.03362293535676031
2213	24	-0.10453089536535742
2213	25	-0.024846025913753067
2214	1	0.32323489254622473
2214	2	0.34261205192904143
2214	3	0.09812819738638723
2214	4	0.16840303724979577
2214	5	0.12417174422338206
2214	6	0.17237710050250854
2214	7	0.22133367706125284
2214	8	0.37603986302392656
2214	9	0.31902567263242076
2214	10	0.11707519063686078
2214	11	0.1536296460236457
2214	12	0.274602199228589
2214	13	0.11488929199211384
2214	14	0.34944033263963803
2214	15	0.24194680824973602
2214	16	0.15224298604366737
2214	17	0.15694716011499565
2214	18	0.4407040894590182
2214	19	0.26610301106968137
2214	20	0.3098333714055741
2214	21	0.20986707777231572
2214	22	0.23728519708924978
2214	23	0.3457657924996198
2214	24	0.48519699579110664
2214	25	0.32505193326991844
2215	1	-0.0659586558408698
2215	2	0.16379484762796537
2215	3	-0.07267825422651697
2215	4	0.06146755337882581
2215	5	0.2724513141158553
2215	6	0.20597925104014791
2215	7	0.08326916093221826
2215	8	0.03652373399167175
2215	9	0.01289664037435756
2215	10	0.010946158378793314
2215	11	-0.12099400989033171
2215	12	0.156376392776977
2215	13	-0.1958096327390706
2215	14	-0.09808654908079006
2215	15	0.3402263781422121
2215	16	-0.1494774440638551
2215	17	0.20200092355585753
2215	18	0.14086537978160152
2215	19	0.10922129063957442
2215	20	0.07961831764212945
2215	21	-0.09658453513090848
2215	22	0.06793035837957007
2215	23	0.05313138389746541
2215	24	0.17917549041476377
2215	25	0.18026698703336308
2216	1	-0.2276203706116675
2216	2	-0.051708123509556
2216	3	-0.031082328929404213
2216	4	-0.020939068013362316
2216	5	0.09320683194268042
2216	6	-0.20503518019923916
2216	7	-0.24156105978084952
2216	8	-0.19698645276554316
2216	9	-0.40682959052547574
2216	10	-0.41864849357367007
2216	11	-0.26661158204652935
2216	12	-0.33151622182403884
2216	13	0.2098673621675502
2216	14	-0.17882721122001044
2216	15	-0.13309705139938188
2216	16	0.052199126394548045
2216	17	-0.23289933111307448
2216	18	-0.05363801580413963
2216	19	-0.0623619012110197
2216	20	0.1531009152652203
2216	21	-0.03059344854347046
2216	22	0.13202203919450994
2216	23	0.15995438899084746
2216	24	-0.048509144559773176
2216	25	-0.17578140006341414
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	305.0
3	64.0
4	30.0
5	35.0
6	26.0
7	102.0
8	75.0
9	172.0
10	237.0
11	312.0
12	485.0
13	862.0
14	1522.0
15	2941.0
16	4156.0
17	4501.0
18	4433.0
19	4137.0
20	3892.0
21	3697.0
22	3120.0
23	2810.0
24	2583.0
25	2294.0
26	2114.0
27	1874.0
28	1764.0
29	1762.0
30	1941.0
31	2436.0
32	2906.0
33	3755.0
34	5230.0
35	8178.0
36	15154.0
37	37233.0
38	220913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.657490948796045	22.774265846790414	9.855180736739268	22.713062467674273
2	10.627470057079368	30.854233170509858	29.40839524367392	29.10990152873686
3	23.05458083496213	36.987791924419895	23.399723080200236	16.557904160417742
4	14.20380625626029	28.173319364012112	22.807603301979114	34.815271077748484
5	9.69712497589033	45.04808979448486	31.700688888824917	13.554096340799896
6	30.238802362123145	32.337891816601626	16.645658505139803	20.77764731613542
7	17.64648322267407	40.92205501017375	20.987846187939436	20.44361557921275
8	27.018574568722453	37.16726318575719	19.962194883362297	15.851967362158057
9	25.83281534175933	24.09639247646016	17.350415544351588	32.72037663742892
10	18.429854354959417	37.850440389154336	25.9518738126763	17.767831443209946
11	22.531268252401766	33.6247004088538	14.158828623794081	29.685202714950353
12	23.864882207879283	39.954362207533975	23.02464037937494	13.156115205211801
13	29.277440674258216	26.08202553799035	18.85700797918274	25.783525808568697
14	20.669103257741455	36.79722573961091	20.904800276274894	21.62887072637274
15	37.025184400694144	23.295354800461613	18.529571741439003	21.14988905740524
16	14.673439141257896	32.56054218167691	32.929191188108845	19.836827488956356
17	27.14846054452896	35.682445401777876	22.390954637935153	14.778139415758016
18	26.075483100476266	26.20613246233759	27.682124922660755	20.03625951452539
19	22.71794768507462	37.585491542199804	27.399014239718717	12.297546533006857
20	24.293094268487472	28.73843408688645	24.4741196955089	22.494351949117178
21	17.129116649062773	34.344573923404354	29.331637219716743	19.19467220781613
22	22.694027402935944	32.65334307762247	16.346236843771038	28.306392675670544
23	15.730857667436954	32.34663543306407	26.60228093551163	25.320225963987347
24	21.198445819542382	41.65980716649877	19.4707152108217	17.67103180313714
25	21.933877702085333	27.621413122679588	17.594638044898854	32.850071130336225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9892.0
1	9892.0
2	8177.0
3	6462.0
4	6462.0
5	6462.0
6	6114.5
7	5767.0
8	5767.0
9	5767.0
10	5268.5
11	4770.0
12	4770.0
13	4770.0
14	4282.0
15	3794.0
16	3794.0
17	3794.0
18	4002.5
19	4211.0
20	4211.0
21	4211.0
22	4871.0
23	5531.0
24	5531.0
25	5531.0
26	6140.5
27	6750.0
28	6750.0
29	6750.0
30	7282.5
31	7815.0
32	7815.0
33	7815.0
34	7743.5
35	7672.0
36	7672.0
37	7672.0
38	7667.0
39	7662.0
40	7662.0
41	7662.0
42	12763.0
43	17864.0
44	17864.0
45	17864.0
46	75149.0
47	132434.0
48	132434.0
49	132434.0
50	108555.0
51	84676.0
52	84676.0
53	84676.0
54	55240.0
55	25804.0
56	25804.0
57	25804.0
58	16123.5
59	6443.0
60	6443.0
61	6443.0
62	5657.0
63	4871.0
64	4871.0
65	4871.0
66	3802.0
67	2733.0
68	2733.0
69	2733.0
70	2152.5
71	1572.0
72	1572.0
73	1572.0
74	1170.0
75	768.0
76	768.0
77	768.0
78	566.0
79	364.0
80	364.0
81	364.0
82	247.5
83	131.0
84	131.0
85	131.0
86	85.5
87	40.0
88	40.0
89	40.0
90	28.5
91	17.0
92	17.0
93	17.0
94	11.0
95	5.0
96	5.0
97	5.0
98	4.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008906740678808566
2	0.18388109788508006
3	0.18905275376309794
4	0.1789967562225076
5	0.19537366650289756
6	0.1629071601575631
7	0.1689407586819173
8	0.1376235091983646
9	0.15945938957221786
10	0.18129526994607112
11	0.1419332224300462
12	0.1528511626169728
13	0.18589229739319812
14	0.16405641701934487
15	0.1649183596656812
16	0.16261984594211767
17	0.16032133221855419
18	0.1597467037876633
19	0.14423173615360968
20	0.16836613025102642
21	0.16951538711280817
22	0.14279516507638249
23	0.16147058908033593
24	0.17267584348270798
25	0.6341024734880808
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	348051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.648416467701573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.97556466274305	15.100660535381309
2	10.325701784118571	3.8511597438306455
3	3.526638523403075	1.9729867174638198
4	1.529904785381937	1.1412120637492782
5	0.7333682556312205	0.6838078327601415
6	0.46528826302653065	0.5206133583871329
7	0.34819585246356266	0.45453108883468224
8	0.23880688996394786	0.3562696271523426
9	0.19258620158382891	0.3232284923761173
>10	1.3065047915446955	4.901867829714611
>50	0.11555172095029735	1.5635639604540712
>100	0.1432841339783687	5.704049119238273
>500	0.033895171478753885	4.430385202168647
>1k	0.04622068838011894	21.81318255083307
>5k	0.01232551690136505	16.938034943154882
>10k+	0.006162758450682525	20.244446934500978
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	24474	7.03172810881164	No Hit
CCCATGTACTCTGCGTTGATACCAC	23428	6.731197439455712	No Hit
GAGTACATGGGAAGCAGTGGTATCA	12474	3.5839575234663883	No Hit
CATGTACTCTGCGTTGATACCACTG	10085	2.897563862767238	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9247	2.656794550223961	No Hit
GCGTTGATACCACTGCTTCCCATGT	8315	2.3890177014288136	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8185	2.3516668534209066	No Hit
GGTATCAACGCAGAGTACATGGGAA	7466	2.145087932515637	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6931	1.991374827252328	No Hit
GTATCAACGCAGAGTACATGGGAAG	6912	1.9859158571588647	No Hit
TATCAACGCAGAGTACATGGGAAGC	6781	1.9482776949355123	No Hit
GTGGTATCAACGCAGAGTACATGGG	5116	1.4698995262188588	No Hit
GTACTCTGCGTTGATACCACTGCTT	4776	1.3722126929674099	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4698	1.3498021841626657	No Hit
GATACCACTGCTTCCCATGTACTCT	4250	1.2210854156431097	No Hit
ATACCACTGCTTCCCATGTACTCTG	3851	1.1064470436803802	No Hit
CAGTGGTATCAACGCAGAGTACATG	3815	1.0961037319243445	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	3602	1.0349058040344663	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	3452	0.9918086717176505	No Hit
CATGGGAAGCAGTGGTATCAACGCA	3300	0.948136910969944	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	3186	0.9153830904091642	No Hit
GTTGATACCACTGCTTCCCATGTAC	3154	0.9061890355149103	No Hit
GCAGTGGTATCAACGCAGAGTACAT	3041	0.8737225291695757	No Hit
TACCACTGCTTCCCATGTACTCTGC	2956	0.8493008208567135	No Hit
ACATGGGAAGCAGTGGTATCAACGC	2833	0.8139611723569248	No Hit
CCACTGCTTCCCATGTACTCTGCGT	2791	0.8018939753082164	No Hit
CTTCCCATGTACTCTGCGTTGATAC	2632	0.7562110150523917	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2374	0.6820839474674689	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	2231	0.6409980146587713	No Hit
CCATGTACTCTGCGTTGATACCACT	2113	0.6070949372362096	No Hit
TGATACCACTGCTTCCCATGTACTC	2092	0.6010613387118555	No Hit
TTCCCATGTACTCTGCGTTGATACC	1869	0.5369902686675229	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1715	0.49274387948892545	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1684	0.4838371388101169	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	1352	0.3884488192822316	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1344	0.38615030555866814	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1309	0.3760943080180778	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	1265	0.36345248253847856	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1077	0.3094374100347363	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1077	0.3094374100347363	No Hit
ACCATGTACTCTGCGTTGATACCAC	1056	0.3034038115103821	No Hit
CTGCGTTGATACCACTGCTTCCCAT	1026	0.294784385047019	No Hit
CTCTGCGTTGATACCACTGCTTCCC	990	0.2844410732909832	No Hit
TCAACGCAGAGTACATGGGAAGCAG	932	0.26777684879514785	No Hit
AAAAAGTACTCTGCGTTGATACCAC	889	0.255422337530994	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	869	0.24967605322208528	No Hit
TCCATGTACTCTGCGTTGATACCAC	785	0.2255416591246685	No Hit
AGTGGTATCAACGCAGAGTACATGG	759	0.21807148952308714	No Hit
AACGCAGAGTACATGGGAAGCAGTG	740	0.21261251942962384	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	739	0.2123252052141784	No Hit
AAAGTACTCTGCGTTGATACCACTG	705	0.2025565218890335	No Hit
ATGTACTCTGCGTTGATACCACTGC	679	0.1950863522874521	No Hit
TCCCATGTACTCTGCGTTGATACCA	676	0.1942244096411158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	659	0.18934006797854339	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	657	0.18876543954765249	No Hit
GTACATGGGTAAGCAGTGGTATCAA	647	0.18589229739319812	No Hit
CCCCATGTACTCTGCGTTGATACCA	642	0.18445572631597096	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	630	0.1810079557306257	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	623	0.1789967562225076	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	596	0.1712392724054808	No Hit
TTGATACCACTGCTTCCCATGTACT	588	0.1689407586819173	No Hit
GAGTACATGGTAAGCAGTGGTATCA	573	0.16463104545023574	No Hit
CAACGCAGAGTACATGGGAAGCAGT	539	0.15486236212509086	No Hit
GTACATGGAAAGCAGTGGTATCAAC	503	0.1445190503690551	No Hit
GCTTACCATGTACTCTGCGTTGATA	481	0.13819813762925548	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	465	0.13360111018212847	No Hit
CGTTGATACCACTGCTTCCCATGTA	460	0.1321645391049013	No Hit
ACTCTGCGTTGATACCACTGCTTAC	449	0.12900408273500147	No Hit
GAGTACATGGAAAGCAGTGGTATCA	413	0.11866077097896573	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	410	0.1177988283326294	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	399	0.11463837196272961	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	380	0.10917940186926628	No Hit
TGTACTCTGCGTTGATACCACTGCT	378	0.10860477343837541	No Hit
GCGTTGATACCACTGCTTACCATGT	376	0.10803014500748453	No Hit
GTACATGGAAGCAGTGGTATCAACG	359	0.1031458033449121	No Hit
CACTGCTTCCCATGTACTCTGCGTT	358	0.10285848912946667	No Hit
CTGCTTACCATGTACTCTGCGTTGA	352	0.10113460383679403	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	351	0.10084728962134859	No Hit
GCTTTCCATGTACTCTGCGTTGATA	351	0.10084728962134859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.873142154454376E-4	0.0
13	0.0	0.0	0.0	5.746284308908752E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	55	5.8766454E-7	17.27286	1
GAACAAA	305	0.0	16.508322	1
AGAACAA	40	0.0052679162	14.250108	2
TACAAAA	115	2.5465852E-11	14.043585	2
GGAAACA	60	4.0775558E-4	12.666762	1
CGCAAAA	70	1.0861243E-4	12.214377	2
AAAAGTA	180	3.45608E-11	10.555635	2
AAAAAGT	180	3.45608E-11	10.555635	1
GAAAAAA	875	0.0	9.1200695	1
AAGTACT	210	6.530172E-10	9.04899	4
AAAGTAC	210	6.548362E-10	9.047688	3
GAAAACA	185	6.477785E-8	8.729795	1
GAAACAA	120	1.7526501E-4	8.7084	1
ACGCAAA	100	0.002892546	8.550065	1
AGTACTC	215	9.515134E-9	8.396622	5
TAAAAAA	205	3.79805E-8	8.341527	1
ATGGGTA	190	7.743709E-6	7.5011373	5
GCAAAAA	165	8.4127634E-5	7.4849052	3
GGGTAAG	190	6.2822015E-5	6.999045	7
CCATGTA	7250	0.0	6.984191	2
>>END_MODULE