FastQCFastQC Report
Wed 25 May 2016
SRR1294837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348051
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC248957.152687393514169No Hit
CCCATGTACTCTGCGTTGATACCAC245737.060172216140738No Hit
GAGTACATGGGAAGCAGTGGTATCA129123.7098011498314905No Hit
CATGTACTCTGCGTTGATACCACTG106533.0607583371402467No Hit
ACGCAGAGTACATGGGAAGCAGTGG89752.5786450836228023No Hit
GCGTTGATACCACTGCTTCCCATGT86292.479234365078681No Hit
GGTATCAACGCAGAGTACATGGGAA74872.1511215310399914No Hit
ACTCTGCGTTGATACCACTGCTTCC74062.127849079588911No Hit
TATCAACGCAGAGTACATGGGAAGC71612.0574570968047783No Hit
GTATCAACGCAGAGTACATGGGAAG70872.0361958448618163No Hit
GTATCAACGCAGAGTACTTTTTTTT57981.665847821152647No Hit
GTGGTATCAACGCAGAGTACATGGG52551.5098362021657745No Hit
GGTATCAACGCAGAGTACTTTTTTT49591.424791194393925No Hit
GCTTCCCATGTACTCTGCGTTGATA48061.380832119430773No Hit
GTACTCTGCGTTGATACCACTGCTT46201.3273916753579216No Hit
TATCAACGCAGAGTACTTTTTTTTT43261.2429212960169629No Hit
GATACCACTGCTTCCCATGTACTCT42251.2139025602569737No Hit
GGGAAGCAGTGGTATCAACGCAGAG40521.164197200984913No Hit
ATACCACTGCTTCCCATGTACTCTG39611.1380516073793783No Hit
CAGTGGTATCAACGCAGAGTACATG39611.1380516073793783No Hit
CATGGGAAGCAGTGGTATCAACGCA37111.0662230535180188No Hit
CTGCTTCCCATGTACTCTGCGTTGA34881.0021519834736863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34800.9998534697501229No Hit
GCAGAGTACATGGGAAGCAGTGGTA34250.9840511879006237No Hit
GCAGTGGTATCAACGCAGAGTACAT31410.9024539507141195No Hit
ACATGGGAAGCAGTGGTATCAACGC30980.8900994394499657No Hit
GTTGATACCACTGCTTCCCATGTAC30250.8691255017224487No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29630.8513120203648316No Hit
TACCACTGCTTCCCATGTACTCTGC28610.8220059703893969No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28200.810226087556134No Hit
CTTCCCATGTACTCTGCGTTGATAC27980.8039051748163344No Hit
CCACTGCTTCCCATGTACTCTGCGT27750.7972969478610894No Hit
AAGCAGTGGTATCAACGCAGAGTAC26130.7507520449589284No Hit
ATGGGAAGCAGTGGTATCAACGCAG25140.7223079376298301No Hit
CCATGTACTCTGCGTTGATACCACT23600.6780615484512327No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21880.6286435033946175No Hit
TGATACCACTGCTTCCCATGTACTC20640.5930165406793831No Hit
TTCCCATGTACTCTGCGTTGATACC18540.5326805554358413No Hit
ATCAACGCAGAGTACATGGGAAGCA17020.4890087946881348No Hit
GGAAGCAGTGGTATCAACGCAGAGT14540.41775486925766625No Hit
GTACATGGTAAGCAGTGGTATCAAC14130.4059749864244034No Hit
ACCATGTACTCTGCGTTGATACCAC11680.3355830036402711No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11530.33127329040858955No Hit
GTGGTATCAACGCAGAGTACTTTTT11040.31719489385176314No Hit
CTGCGTTGATACCACTGCTTCCCAT10350.2973702129860279No Hit
TGGGAAGCAGTGGTATCAACGCAGA9850.283004502213756No Hit
CTCTGCGTTGATACCACTGCTTCCC9770.2807059884901925No Hit
TCAACGCAGAGTACATGGGAAGCAG9650.2772582179048473No Hit
AAAAAGTACTCTGCGTTGATACCAC8630.24795216792941263No Hit
ATCAACGCAGAGTACTTTTTTTTTT8090.232437200295359No Hit
AGTGGTATCAACGCAGAGTACATGG8050.23128794343357725No Hit
TCCATGTACTCTGCGTTGATACCAC7720.22180657432387782No Hit
GGTAAGCAGTGGTATCAACGCAGAG7610.218646117953978No Hit
AACGCAGAGTACATGGGAAGCAGTG7450.21404909050685103No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7420.21318714786051468No Hit
GTACATGGGTAAGCAGTGGTATCAA7070.20313115031992435No Hit
CCCCATGTACTCTGCGTTGATACCA6930.19910875130368824No Hit
AAAGTACTCTGCGTTGATACCACTG6820.19594829493378846No Hit
ACTGCTTCCCATGTACTCTGCGTTG6640.19077663905577058No Hit
TCCCATGTACTCTGCGTTGATACCA6610.18991469640943426No Hit
ATGTACTCTGCGTTGATACCACTGC6430.18474304053141638No Hit
GAGTACATGGTAAGCAGTGGTATCA6080.17468704299082607No Hit
CAACGCAGAGTACATGGGAAGCAGT5970.17152658662092624No Hit
GTACATGGAAAGCAGTGGTATCAAC5830.16750418760469013No Hit
TTGATACCACTGCTTCCCATGTACT5790.16635493074290839No Hit
GCTTACCATGTACTCTGCGTTGATA5260.15112727732430017No Hit
CGTTGATACCACTGCTTCCCATGTA4990.14336979350727336No Hit
CGCAGAGTACATGGGAAGCAGTGGT4980.14308247929182794No Hit
ACTCTGCGTTGATACCACTGCTTAC4530.1301533395967832No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4460.12814214008866515No Hit
ACGCAGAGTACATGGTAAGCAGTGG4130.11866077097896573No Hit
TGTACTCTGCGTTGATACCACTGCT3900.11205254402372065No Hit
GCGTTGATACCACTGCTTACCATGT3890.11176522980827523No Hit
GAGTACATGGAAAGCAGTGGTATCA3860.11090328716193891No Hit
CTGCTTACCATGTACTCTGCGTTGA3830.11004134451560259No Hit
CAACGCAGAGTACTTTTTTTTTTTT3750.10774283079203911No Hit
CACTGCTTCCCATGTACTCTGCGTT3720.10688088814570279No Hit
GTACATGGAAGCAGTGGTATCAACG3590.1031458033449121No Hit
GCTTTCCATGTACTCTGCGTTGATA3560.10228386069857577No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3500.10055997540590317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA755.5282726E-815.2596181
GAAAACA1051.868284E-812.7163491
GAAAAAA3950.09.4165371
ATGGAAG850.007497148.92853457
AAAAGTA2002.1341657E-78.1066722
ATGGGTA1451.4869122E-47.89062745
AAAAAGT2104.6543755E-77.720641
CATGGGT2052.6893704E-67.44156744
CCATGTA72150.06.97945172
CATGGTA3601.4551915E-106.88603354
TGCGTTG77050.06.808659612
TACTCTG77250.06.7910327
CTGCGTT77300.06.774366411
TCTGCGT78400.06.679318410
CTCTGCG78550.06.6665639
TGATACC80500.06.528644617
TTGATAC80550.06.524591416
ACTCTGC81050.06.4726368
CGTTGAT81500.06.43689814
TGTACTC78950.06.4126965
Produced by FastQC (version 0.11.5)