##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294837_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 348051 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02240188937828 33.0 31.0 34.0 30.0 34.0 2 32.12453634668483 33.0 31.0 34.0 30.0 34.0 3 32.28298439021867 34.0 31.0 34.0 30.0 34.0 4 35.59810200229277 37.0 35.0 37.0 35.0 37.0 5 35.748918980264385 37.0 35.0 37.0 35.0 37.0 6 35.61176666637935 37.0 37.0 37.0 35.0 37.0 7 35.73559047380987 37.0 37.0 37.0 35.0 37.0 8 35.70723830702972 37.0 37.0 37.0 35.0 37.0 9 37.444426247877466 39.0 39.0 39.0 35.0 39.0 10 37.52719285392083 39.0 39.0 39.0 35.0 39.0 11 37.53214615099511 39.0 39.0 39.0 35.0 39.0 12 37.59529206926571 39.0 39.0 39.0 35.0 39.0 13 37.41457430089269 39.0 39.0 39.0 35.0 39.0 14 38.85959528919612 41.0 39.0 41.0 36.0 41.0 15 38.76357775153641 41.0 39.0 41.0 36.0 41.0 16 38.60573019471284 41.0 39.0 41.0 35.0 41.0 17 38.80537335045726 41.0 39.0 41.0 36.0 41.0 18 38.776690772329346 41.0 39.0 41.0 36.0 41.0 19 38.69440398102577 41.0 39.0 41.0 34.0 41.0 20 38.80398562279666 41.0 39.0 41.0 35.0 41.0 21 38.73026654139767 41.0 39.0 41.0 35.0 41.0 22 38.85653539280163 41.0 39.0 41.0 35.0 41.0 23 38.77771935722064 41.0 39.0 41.0 35.0 41.0 24 38.78883841735838 41.0 39.0 41.0 35.0 41.0 25 37.967524874228204 41.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.47325134753368303 1101 2 -0.4904664017048468 1101 3 -0.5346132048064689 1101 4 -0.5183614072358225 1101 5 -0.32585509974075677 1101 6 -0.5669415224196257 1101 7 -0.27274863941444494 1101 8 -0.4446311775531484 1101 9 -0.5065908202653802 1101 10 -0.43209794630908505 1101 11 -0.4860850564440753 1101 12 -0.30003830583522983 1101 13 -0.5229507887790206 1101 14 -0.574747915029036 1101 15 -0.63753181757113 1101 16 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2 -0.23255356742176758 1103 3 -0.17851034909849517 1103 4 -0.35132820876999205 1103 5 0.011325804965025554 1103 6 -0.058632793292694885 1103 7 -0.08330637526044171 1103 8 -0.04703935222478606 1103 9 0.031733015641776774 1103 10 -0.09624951632071088 1103 11 0.023953054762884562 1103 12 0.01611865887646502 1103 13 -0.013483738165234627 1103 14 -0.03836001503348996 1103 15 0.006089921612137061 1103 16 -0.04812383374333962 1103 17 -0.18487883917148906 1103 18 -0.20808278698017801 1103 19 -0.05041610478036063 1103 20 -0.05583896137373756 1103 21 -0.1378909269091082 1103 22 -0.09170452432866227 1103 23 -0.12443072139794964 1103 24 -0.3165589278410863 1103 25 -0.09876534279042204 1104 1 -0.21029309403046526 1104 2 -0.2760162199928864 1104 3 -0.1632635531951152 1104 4 -0.23888154160816555 1104 5 -0.20321456293871876 1104 6 -0.29679641060018724 1104 7 -0.40657971172979046 1104 8 -0.18638970255035048 1104 9 -0.30338701934952184 1104 10 -0.23591346786106726 1104 11 -0.15306245199192148 1104 12 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22 0.6821786949705952 2210 23 0.7018696348928444 2210 24 0.614628252697095 2210 25 0.6222663239988648 2211 1 0.0629814225264056 2211 2 0.10953287906990283 2211 3 0.1734173047286589 2211 4 -0.03065476483102003 2211 5 0.19287172772069994 2211 6 0.19977057530870468 2211 7 -0.19324188409127885 2211 8 -0.19728961388553046 2211 9 -0.1106069740645097 2211 10 -0.06475005345926377 2211 11 -0.1160760301671857 2211 12 -0.11435592289813457 2211 13 -0.1277508981012616 2211 14 -0.1478050179178041 2211 15 -0.3189186276992544 2211 16 -0.18004229921966441 2211 17 -0.22799154845690595 2211 18 -0.1951016507335126 2211 19 -0.0775804622693883 2211 20 0.12048756359888557 2211 21 -0.1916980475355743 2211 22 0.060051797843932775 2211 23 0.07563352797251355 2211 24 0.003447842522682265 2211 25 0.3239333380249221 2212 1 0.14978738065354236 2212 2 0.2164392783815856 2212 3 0.22517258460320022 2212 4 0.23648766516220832 2212 5 0.04519999831389043 2212 6 0.009277095442705274 2212 7 0.14895073598353292 2212 8 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19 0.0992336959683442 2213 20 -0.10916384162169379 2213 21 0.031814825214226516 2213 22 0.16647685473183316 2213 23 0.0161722653588825 2213 24 0.1599938234369347 2213 25 0.1586814228981055 2214 1 0.20352141085098197 2214 2 0.10429061403779372 2214 3 0.17759418739066035 2214 4 0.2009301738730045 2214 5 -0.026652498782510747 2214 6 0.1870180942812638 2214 7 0.07127942355612049 2214 8 0.00932159452077741 2214 9 -0.013414913352306712 2214 10 0.031659753896249754 2214 11 0.23671439598576427 2214 12 0.10120741627291352 2214 13 0.03703894428052479 2214 14 0.05601868319136116 2214 15 -0.04672072927544235 2214 16 0.01783277375172787 2214 17 -0.02431670899397176 2214 18 -0.04518337869615152 2214 19 0.0038935599139193755 2214 20 0.12940758694973198 2214 21 0.10392366875164072 2214 22 0.18620474588829694 2214 23 0.212600836787459 2214 24 0.25495980983149735 2214 25 -0.024583883224345016 2215 1 0.19873646461442718 2215 2 0.28633362479048685 2215 3 0.1716264454551748 2215 4 0.2546398512923602 2215 5 0.210390511970175 2215 6 0.15900734159309593 2215 7 0.1989675955991359 2215 8 0.14883772355303648 2215 9 0.10766035546489405 2215 10 0.12198233454141416 2215 11 0.1853165465234028 2215 12 0.0274439754126945 2215 13 -0.10473524926786126 2215 14 -0.01925013401294251 2215 15 0.10408572233746582 2215 16 0.06503707482699639 2215 17 0.1918660867049482 2215 18 0.12245102990599577 2215 19 0.12577528034402974 2215 20 0.12800973748736766 2215 21 0.3119881848806756 2215 22 0.14641979965173846 2215 23 0.13491266474444785 2215 24 0.1432393797239655 2215 25 -0.24705700150391863 2216 1 0.3637407090965965 2216 2 0.22986078947639044 2216 3 0.2771336610748705 2216 4 0.23007491071511055 2216 5 0.2585229398139788 2216 6 0.1390794977900356 2216 7 0.13323117794208628 2216 8 0.042545278731303426 2216 9 0.2214973673494427 2216 10 0.2316597538962526 2216 11 0.06903895738927446 2216 12 0.08230390750098593 2216 13 0.10519683901736698 2216 14 -0.009880439615656655 2216 15 0.04637137598771801 2216 16 -0.042518103441253174 2216 17 -0.044031621274676525 2216 18 0.12977276165472773 2216 19 -0.04770731727906252 2216 20 0.22456109572166127 2216 21 0.13122630033058869 2216 22 -0.04614174533977433 2216 23 -0.04014039128271918 2216 24 -0.0511366813965779 2216 25 -0.06598739199627346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 16.0 9 73.0 10 128.0 11 139.0 12 186.0 13 221.0 14 395.0 15 671.0 16 909.0 17 1085.0 18 1246.0 19 1329.0 20 1367.0 21 1520.0 22 1354.0 23 1260.0 24 1183.0 25 1323.0 26 1304.0 27 1264.0 28 1245.0 29 1417.0 30 1698.0 31 2388.0 32 2902.0 33 4320.0 34 6333.0 35 10678.0 36 19196.0 37 47280.0 38 233619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.922336374739565 19.14390401609311 10.832962138084632 23.100797471082693 2 11.520182516148312 26.159977472817967 31.961014182929915 30.358825828103807 3 24.59639535585302 32.243263199933345 26.37285914995216 16.787482294261473 4 14.929708577191272 23.907128552999417 25.497700049705358 35.66546282010395 5 10.228820406278855 40.513042474607566 34.85543628808864 14.402700831024932 6 32.68169993819974 27.422641889075727 17.946506848330674 21.949151324393853 7 18.25514039100302 36.63728205776547 23.658441274839955 21.449136276391556 8 29.53618866200643 31.286506862500037 22.21599708088757 16.961307394605964 9 27.913437973170595 16.908154264748557 20.278637326138984 34.89977043594186 10 20.022123258152565 32.28559115069674 28.956759086338167 18.735526504812526 11 24.38407015063884 27.554869832294692 16.39529839017845 31.665761626888013 12 26.049056029145156 34.758124527727254 26.152776460920958 13.040042982206632 13 32.08753889516191 19.614654174244578 22.35764298910217 25.940163941491335 14 22.585483161950403 30.77939727223884 25.070463811337994 21.564655754472764 15 39.10403935055495 17.06043079893464 22.66391994276701 21.171609907743406 16 14.949245943841563 26.969036147001447 37.42123999069102 20.660477918465972 17 27.264917482646073 30.56819749000138 26.77073277249115 15.396152254861398 18 26.270575289253586 19.935296838681687 33.162668689358746 20.63145918270598 19 22.68144610990918 31.8585494654519 33.492218094474666 11.967786330164259 20 24.042166234258772 22.599274244291784 30.780833843316064 22.577725678133376 21 16.300050567737784 29.073001425090794 35.36466234542362 19.262285661747804 22 22.06742192864757 27.44169609276908 22.610452036650223 27.880429941933127 23 14.956141485012253 26.42400108030145 33.26236672211831 25.357490712567987 24 20.779090180349204 36.213499900875455 25.770370093694243 17.237039825081098 25 21.67589928160961 22.11802990274851 23.81180988958233 32.394260926059545 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3913.0 1 3913.0 2 3460.0 3 3007.0 4 3007.0 5 3007.0 6 3904.5 7 4802.0 8 4802.0 9 4802.0 10 4237.5 11 3673.0 12 3673.0 13 3673.0 14 2630.0 15 1587.0 16 1587.0 17 1587.0 18 2227.0 19 2867.0 20 2867.0 21 2867.0 22 4258.5 23 5650.0 24 5650.0 25 5650.0 26 7399.0 27 9148.0 28 9148.0 29 9148.0 30 9623.0 31 10098.0 32 10098.0 33 10098.0 34 10113.5 35 10129.0 36 10129.0 37 10129.0 38 8800.5 39 7472.0 40 7472.0 41 7472.0 42 12609.5 43 17747.0 44 17747.0 45 17747.0 46 76710.5 47 135674.0 48 135674.0 49 135674.0 50 112179.0 51 88684.0 52 88684.0 53 88684.0 54 57904.5 55 27125.0 56 27125.0 57 27125.0 58 16782.5 59 6440.0 60 6440.0 61 6440.0 62 5546.0 63 4652.0 64 4652.0 65 4652.0 66 3664.5 67 2677.0 68 2677.0 69 2677.0 70 2099.0 71 1521.0 72 1521.0 73 1521.0 74 1145.0 75 769.0 76 769.0 77 769.0 78 528.5 79 288.0 80 288.0 81 288.0 82 183.0 83 78.0 84 78.0 85 78.0 86 57.5 87 37.0 88 37.0 89 37.0 90 22.5 91 8.0 92 8.0 93 8.0 94 6.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02183588037385326 2 0.007757483817026815 3 0.0 4 0.0 5 0.42838549522914743 6 0.04482101760948826 7 0.006608226955245064 8 0.0 9 0.0 10 2.873142154454376E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 8.619426463363128E-4 18 0.0 19 0.0 20 0.0 21 8.619426463363128E-4 22 0.0011492568617817503 23 0.0 24 0.0011492568617817503 25 0.05458970093463314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 348051.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.02193644034926 #Duplication Level Percentage of deduplicated Percentage of total 1 79.12554509983933 11.886189092977753 2 11.596281845306404 3.4839721764913762 3 3.685639966337694 1.6609634794900747 4 1.711804758625966 1.0285848912946667 5 0.8434702777140235 0.63352784505719 6 0.5470124703542193 0.4930311937043709 7 0.3595746308622141 0.37810550752619587 8 0.2696809731466605 0.3240904350224536 9 0.19700099456812795 0.2663402777179206 >10 1.2412975288807284 3.7281892596199984 >50 0.13962206411139164 1.475358496312322 >100 0.1568357432484125 5.308417444569905 >500 0.0401652513197154 4.415157548750039 >1k 0.06311682350240991 27.32846623052369 >5k 0.015301048121796343 16.606187024315403 >10k+ 0.007650524060898172 20.983419096626644 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 24895 7.152687393514169 No Hit CCCATGTACTCTGCGTTGATACCAC 24573 7.060172216140738 No Hit GAGTACATGGGAAGCAGTGGTATCA 12912 3.7098011498314905 No Hit CATGTACTCTGCGTTGATACCACTG 10653 3.0607583371402467 No Hit ACGCAGAGTACATGGGAAGCAGTGG 8975 2.5786450836228023 No Hit GCGTTGATACCACTGCTTCCCATGT 8629 2.479234365078681 No Hit GGTATCAACGCAGAGTACATGGGAA 7487 2.1511215310399914 No Hit ACTCTGCGTTGATACCACTGCTTCC 7406 2.127849079588911 No Hit TATCAACGCAGAGTACATGGGAAGC 7161 2.0574570968047783 No Hit GTATCAACGCAGAGTACATGGGAAG 7087 2.0361958448618163 No Hit GTATCAACGCAGAGTACTTTTTTTT 5798 1.665847821152647 No Hit GTGGTATCAACGCAGAGTACATGGG 5255 1.5098362021657745 No Hit GGTATCAACGCAGAGTACTTTTTTT 4959 1.424791194393925 No Hit GCTTCCCATGTACTCTGCGTTGATA 4806 1.380832119430773 No Hit GTACTCTGCGTTGATACCACTGCTT 4620 1.3273916753579216 No Hit TATCAACGCAGAGTACTTTTTTTTT 4326 1.2429212960169629 No Hit GATACCACTGCTTCCCATGTACTCT 4225 1.2139025602569737 No Hit GGGAAGCAGTGGTATCAACGCAGAG 4052 1.164197200984913 No Hit ATACCACTGCTTCCCATGTACTCTG 3961 1.1380516073793783 No Hit CAGTGGTATCAACGCAGAGTACATG 3961 1.1380516073793783 No Hit CATGGGAAGCAGTGGTATCAACGCA 3711 1.0662230535180188 No Hit CTGCTTCCCATGTACTCTGCGTTGA 3488 1.0021519834736863 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3480 0.9998534697501229 No Hit GCAGAGTACATGGGAAGCAGTGGTA 3425 0.9840511879006237 No Hit GCAGTGGTATCAACGCAGAGTACAT 3141 0.9024539507141195 No Hit ACATGGGAAGCAGTGGTATCAACGC 3098 0.8900994394499657 No Hit GTTGATACCACTGCTTCCCATGTAC 3025 0.8691255017224487 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2963 0.8513120203648316 No Hit TACCACTGCTTCCCATGTACTCTGC 2861 0.8220059703893969 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2820 0.810226087556134 No Hit CTTCCCATGTACTCTGCGTTGATAC 2798 0.8039051748163344 No Hit CCACTGCTTCCCATGTACTCTGCGT 2775 0.7972969478610894 No Hit AAGCAGTGGTATCAACGCAGAGTAC 2613 0.7507520449589284 No Hit ATGGGAAGCAGTGGTATCAACGCAG 2514 0.7223079376298301 No Hit CCATGTACTCTGCGTTGATACCACT 2360 0.6780615484512327 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2188 0.6286435033946175 No Hit TGATACCACTGCTTCCCATGTACTC 2064 0.5930165406793831 No Hit TTCCCATGTACTCTGCGTTGATACC 1854 0.5326805554358413 No Hit ATCAACGCAGAGTACATGGGAAGCA 1702 0.4890087946881348 No Hit GGAAGCAGTGGTATCAACGCAGAGT 1454 0.41775486925766625 No Hit GTACATGGTAAGCAGTGGTATCAAC 1413 0.4059749864244034 No Hit ACCATGTACTCTGCGTTGATACCAC 1168 0.3355830036402711 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1153 0.33127329040858955 No Hit GTGGTATCAACGCAGAGTACTTTTT 1104 0.31719489385176314 No Hit CTGCGTTGATACCACTGCTTCCCAT 1035 0.2973702129860279 No Hit TGGGAAGCAGTGGTATCAACGCAGA 985 0.283004502213756 No Hit CTCTGCGTTGATACCACTGCTTCCC 977 0.2807059884901925 No Hit TCAACGCAGAGTACATGGGAAGCAG 965 0.2772582179048473 No Hit AAAAAGTACTCTGCGTTGATACCAC 863 0.24795216792941263 No Hit ATCAACGCAGAGTACTTTTTTTTTT 809 0.232437200295359 No Hit AGTGGTATCAACGCAGAGTACATGG 805 0.23128794343357725 No Hit TCCATGTACTCTGCGTTGATACCAC 772 0.22180657432387782 No Hit GGTAAGCAGTGGTATCAACGCAGAG 761 0.218646117953978 No Hit AACGCAGAGTACATGGGAAGCAGTG 745 0.21404909050685103 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 742 0.21318714786051468 No Hit GTACATGGGTAAGCAGTGGTATCAA 707 0.20313115031992435 No Hit CCCCATGTACTCTGCGTTGATACCA 693 0.19910875130368824 No Hit AAAGTACTCTGCGTTGATACCACTG 682 0.19594829493378846 No Hit ACTGCTTCCCATGTACTCTGCGTTG 664 0.19077663905577058 No Hit TCCCATGTACTCTGCGTTGATACCA 661 0.18991469640943426 No Hit ATGTACTCTGCGTTGATACCACTGC 643 0.18474304053141638 No Hit GAGTACATGGTAAGCAGTGGTATCA 608 0.17468704299082607 No Hit CAACGCAGAGTACATGGGAAGCAGT 597 0.17152658662092624 No Hit GTACATGGAAAGCAGTGGTATCAAC 583 0.16750418760469013 No Hit TTGATACCACTGCTTCCCATGTACT 579 0.16635493074290839 No Hit GCTTACCATGTACTCTGCGTTGATA 526 0.15112727732430017 No Hit CGTTGATACCACTGCTTCCCATGTA 499 0.14336979350727336 No Hit CGCAGAGTACATGGGAAGCAGTGGT 498 0.14308247929182794 No Hit ACTCTGCGTTGATACCACTGCTTAC 453 0.1301533395967832 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 446 0.12814214008866515 No Hit ACGCAGAGTACATGGTAAGCAGTGG 413 0.11866077097896573 No Hit TGTACTCTGCGTTGATACCACTGCT 390 0.11205254402372065 No Hit GCGTTGATACCACTGCTTACCATGT 389 0.11176522980827523 No Hit GAGTACATGGAAAGCAGTGGTATCA 386 0.11090328716193891 No Hit CTGCTTACCATGTACTCTGCGTTGA 383 0.11004134451560259 No Hit CAACGCAGAGTACTTTTTTTTTTTT 375 0.10774283079203911 No Hit CACTGCTTCCCATGTACTCTGCGTT 372 0.10688088814570279 No Hit GTACATGGAAGCAGTGGTATCAACG 359 0.1031458033449121 No Hit GCTTTCCATGTACTCTGCGTTGATA 356 0.10228386069857577 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 350 0.10055997540590317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 2.873142154454376E-4 0.0 13 0.0 0.0 0.0 5.746284308908752E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 75 5.5282726E-8 15.259618 1 GAAAACA 105 1.868284E-8 12.716349 1 GAAAAAA 395 0.0 9.416537 1 ATGGAAG 85 0.00749714 8.9285345 7 AAAAGTA 200 2.1341657E-7 8.106672 2 ATGGGTA 145 1.4869122E-4 7.8906274 5 AAAAAGT 210 4.6543755E-7 7.72064 1 CATGGGT 205 2.6893704E-6 7.4415674 4 CCATGTA 7215 0.0 6.9794517 2 CATGGTA 360 1.4551915E-10 6.8860335 4 TGCGTTG 7705 0.0 6.8086596 12 TACTCTG 7725 0.0 6.791032 7 CTGCGTT 7730 0.0 6.7743664 11 TCTGCGT 7840 0.0 6.6793184 10 CTCTGCG 7855 0.0 6.666563 9 TGATACC 8050 0.0 6.5286446 17 TTGATAC 8055 0.0 6.5245914 16 ACTCTGC 8105 0.0 6.472636 8 CGTTGAT 8150 0.0 6.436898 14 TGTACTC 7895 0.0 6.412696 5 >>END_MODULE