FastQCFastQC Report
Wed 25 May 2016
SRR1294836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264715
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC73950.22651288090997224No Hit
CCCATGTACTCTGCGTTGATACCAC65840.20167150884533566No Hit
GAGTACATGGGAAGCAGTGGTATCA35350.10827897687853304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGG601.477134E-615.82393414
CTTCGCG852.7120404E-713.40771213
TACGACC1001.0057192E-813.302724
TAGCGTC655.4463933E-513.1563345
ACCGGAC655.4511038E-513.1551233
TCGCGGG751.4883528E-512.65953615
CGGCTCG701.0935949E-412.2119078
GTACGAC951.0397052E-612.00116353
CCGAATT658.067653E-411.68536614
CCGGCTT907.483679E-611.60885059
CGGGCGA1001.941784E-611.39498118
CGACCAT855.3515647E-511.1713786
TCGTCCG951.3642939E-510.9978589
CGGACGT700.001494971810.85469510
CGAATTA803.79275E-410.68148315
CGTCCGC1451.45046215E-810.48039510
GCGGGAC1201.5232599E-610.2926653
TCGGACG750.002653185710.131369
GTGTTAG4600.09.91430951
ATCGTCG1252.5786394E-69.8780769
Produced by FastQC (version 0.11.5)