FastQCFastQC Report
Wed 25 May 2016
SRR1294836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264715
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT101990.3124009293307379No Hit
GTACATGGGAAGCAGTGGTATCAAC73030.22369487076207267No Hit
CCCATGTACTCTGCGTTGATACCAC69450.21272913562133294No Hit
GGTATCAACGCAGAGTACTTTTTTT65690.20121205066904768No Hit
TATCAACGCAGAGTACTTTTTTTTT57500.17612563424372418No Hit
GAGTACATGGGAAGCAGTGGTATCA35450.1085852823293917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA751.5010573E-512.64907515
CGTAGCA1103.630157E-812.13507652
GTCCGCA951.0585445E-611.98333411
CGTCCGC855.4027238E-511.16094910
ACCGCCG600.00593912411.0679411
GTCTACG951.3038612E-511.0429011
TACGCAG700.001448382410.8959655
TCGTCTA1252.2859967E-710.62522314
TCGTCCG1106.151815E-610.3492439
ACACCGT750.002667389810.1248426
GCTCGTT1901.0186341E-109.98611211
GTGTAGA4600.09.9517061
TCGTCCC1054.1711774E-59.9385589
CGCGTCC1054.1711774E-59.93855810
GTCCCGC2159.094947E-129.7588431
TCCCGCA1701.5705155E-89.5347032
CTACGAC1106.578871E-59.5339693
TAGGACG900.00107841249.5339694
TCGCCCA2600.09.48680612
GCGCGTC1106.9456735E-59.4868069
Produced by FastQC (version 0.11.5)