FastQCFastQC Report
Wed 25 May 2016
SRR1294835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284548
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC157005.51752252695503No Hit
CCCATGTACTCTGCGTTGATACCAC152085.344616725473382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA117464.127950293096419No Hit
GAGTACATGGGAAGCAGTGGTATCA79982.8107735777443525No Hit
CATGTACTCTGCGTTGATACCACTG66702.3440684875662456No Hit
GCGTTGATACCACTGCTTCCCATGT54291.9079382037476982No Hit
ACGCAGAGTACATGGGAAGCAGTGG54151.9030181199657No Hit
GGTATCAACGCAGAGTACATGGGAA49821.7508469572796153No Hit
TATCAACGCAGAGTACATGGGAAGC47051.653499585307224No Hit
ACTCTGCGTTGATACCACTGCTTCC46601.6376850302936588No Hit
GTATCAACGCAGAGTACATGGGAAG44981.5807526322448233No Hit
GTGGTATCAACGCAGAGTACATGGG34561.2145578250418207No Hit
GTACTCTGCGTTGATACCACTGCTT32011.1249420132982837No Hit
GCTTCCCATGTACTCTGCGTTGATA30801.0824184320395855No Hit
GATACCACTGCTTCCCATGTACTCT26760.9404388714733543No Hit
ATACCACTGCTTCCCATGTACTCTG25080.8813978660893769No Hit
CAGTGGTATCAACGCAGAGTACATG23460.8244654680405414No Hit
GGGAAGCAGTGGTATCAACGCAGAG23340.8202482533702574No Hit
CTGCTTCCCATGTACTCTGCGTTGA22320.7844019286728424No Hit
CATGGGAAGCAGTGGTATCAACGCA21940.7710474155502762No Hit
GCAGAGTACATGGGAAGCAGTGGTA20770.7299295725150062No Hit
GTTGATACCACTGCTTCCCATGTAC20480.719737970395153No Hit
TACCACTGCTTCCCATGTACTCTGC19700.6923260750383063No Hit
GCAGTGGTATCAACGCAGAGTACAT19300.678268692804026No Hit
GTATCAACGCAGAGTACTTTTTTTT19260.676862954580598No Hit
ACATGGGAAGCAGTGGTATCAACGC18670.6561283157850345No Hit
CTTCCCATGTACTCTGCGTTGATAC17820.6262563785371887No Hit
CCACTGCTTCCCATGTACTCTGCGT17400.6114961271911945No Hit
AAGCAGTGGTATCAACGCAGAGTAC16340.5742440642703516No Hit
GGTATCAACGCAGAGTACTTTTTTT15590.5478864725810759No Hit
CCATGTACTCTGCGTTGATACCACT15000.5271518337855124No Hit
ATGGGAAGCAGTGGTATCAACGCAG14960.5257460955620844No Hit
AAACAAAAAAAAAAAAAAAAAAAAA13760.48357394885924343No Hit
TGATACCACTGCTTCCCATGTACTC13430.4719766085159622No Hit
TTCCCATGTACTCTGCGTTGATACC12210.4291015927014072No Hit
TATCAACGCAGAGTACTTTTTTTTT12160.4273444199221221No Hit
ATCAACGCAGAGTACATGGGAAGCA11080.38938948788956523No Hit
GTACATGGTAAGCAGTGGTATCAAC10420.3661948072030026No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7920.27833616823875057No Hit
GGAAGCAGTGGTATCAACGCAGAGT7830.2751732572360375No Hit
ACCATGTACTCTGCGTTGATACCAC7740.27201034623332443No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7030.24705849276747682No Hit
AAAAACAAAAAAAAAAAAAAAAAAA6520.2291353304187694No Hit
CTGCGTTGATACCACTGCTTCCCAT6340.22280950841334327No Hit
CTCTGCGTTGATACCACTGCTTCCC6190.21753799007548816No Hit
TCAACGCAGAGTACATGGGAAGCAG6160.21648368640791713No Hit
TGGGAAGCAGTGGTATCAACGCAGA5970.20980642984663395No Hit
AAAAAGTACTCTGCGTTGATACCAC5890.2069949533997779No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4980.17501440881679012No Hit
AGTGGTATCAACGCAGAGTACATGG4970.17466297426093313No Hit
CCCCATGTACTCTGCGTTGATACCA4940.1736086705933621No Hit
AACGCAGAGTACATGGGAAGCAGTG4940.1736086705933621No Hit
AAAGTACTCTGCGTTGATACCACTG4840.17009432503479202No Hit
ATGTACTCTGCGTTGATACCACTGC4780.16798571769964998No Hit
ACACAAAAAAAAAAAAAAAAAAAAA4690.16482280669693689No Hit
GGTAAGCAGTGGTATCAACGCAGAG4680.16447137214107987No Hit
TCCATGTACTCTGCGTTGATACCAC4570.1606055920266528No Hit
GAGTACATGGTAAGCAGTGGTATCA4420.15533407368879767No Hit
TCCCATGTACTCTGCGTTGATACCA4330.1521711626860846No Hit
ACTGCTTCCCATGTACTCTGCGTTG4240.1490082516833715No Hit
GAACAAAAAAAAAAAAAAAAAAAAA3990.14022238778694632No Hit
GCTTACCATGTACTCTGCGTTGATA3890.13670804222837624No Hit
TTGATACCACTGCTTCCCATGTACT3740.1314365238905211No Hit
GTACATGGAAAGCAGTGGTATCAAC3700.13003078566709306No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA3630.127570743776094No Hit
GTACATGGGTAAGCAGTGGTATCAA3550.12475926732923796No Hit
GTGGTATCAACGCAGAGTACTTTTT3440.12089348721481086No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3360.1180820107679548No Hit
CGTTGATACCACTGCTTCCCATGTA3240.11386479609767068No Hit
CAACGCAGAGTACATGGGAAGCAGT3220.11316192698595667No Hit
ACTCTGCGTTGATACCACTGCTTAC3200.11245905787424265No Hit
CGCAGAGTACATGGGAAGCAGTGGT3190.11210762331838565No Hit
GCGTTGATACCACTGCTTACCATGT3020.1061332358688165No Hit
ACGCAGAGTACATGGTAAGCAGTGG2970.10437606308953146No Hit
GGTATCAACGCAGAGTACATGGGGG2920.10261889031024643No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA2880.1012131520868184No Hit
GAGTACATGGAAAGCAGTGGTATCA2850.10015884841924738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA502.1579399E-718.9998231
GAACAAA2500.014.4398671
GGAGTAT400.00525923714.2523775
GGAAACA551.9514457E-413.8180541
AGAACAA752.0626414E-411.3998952
TACAAAA1104.913727E-711.2271692
GAGAACA855.2877127E-511.1763681
ACGCAAA750.002636400710.133241
CGCAAAA750.002636400710.133242
TCCTGAA856.558238E-410.058732
AAAGTAC1606.6440407E-78.9061671
ATCCTGA1304.32824E-58.769151
GAAACAA1304.32824E-58.769152
CAAAAAC1651.0323474E-68.6378034
AGTACTC1701.4341067E-57.82345723
GAAAACA1356.2257936E-47.7406691
AAGTACT1752.1095755E-57.59993032
GAAGCAG45150.07.19467979
CACAAAA4800.07.12493372
CAGTGGT49100.07.0982513
Produced by FastQC (version 0.11.5)