##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294835_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 284548 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.017318694912632 33.0 31.0 34.0 16.0 34.0 2 30.270467548533112 33.0 31.0 34.0 20.0 34.0 3 30.658532128147097 34.0 31.0 34.0 23.0 34.0 4 33.32034314070034 37.0 35.0 37.0 19.0 37.0 5 34.24808116732502 37.0 35.0 37.0 29.0 37.0 6 33.895160043296734 37.0 35.0 37.0 26.0 37.0 7 34.18315363313044 37.0 35.0 37.0 28.0 37.0 8 33.97763470486526 37.0 35.0 37.0 26.0 37.0 9 35.328854885643196 39.0 37.0 39.0 23.0 39.0 10 35.640633566217296 39.0 37.0 39.0 27.0 39.0 11 35.52865597368458 39.0 37.0 39.0 27.0 39.0 12 35.75000351434556 39.0 37.0 39.0 27.0 39.0 13 35.344086059294035 39.0 37.0 39.0 25.0 39.0 14 36.80821161983216 41.0 38.0 41.0 26.0 41.0 15 36.69764327986842 41.0 38.0 41.0 25.0 41.0 16 36.67446617090965 40.0 38.0 41.0 26.0 41.0 17 36.7472517817732 40.0 38.0 41.0 27.0 41.0 18 36.66617231539143 41.0 37.0 41.0 27.0 41.0 19 36.61458875128274 40.0 37.0 41.0 27.0 41.0 20 36.60929614687153 40.0 37.0 41.0 26.0 41.0 21 36.57423703557924 40.0 37.0 41.0 26.0 41.0 22 36.63377356368697 41.0 37.0 41.0 26.0 41.0 23 36.65488775180286 41.0 37.0 41.0 26.0 41.0 24 36.80027271321534 41.0 37.0 41.0 27.0 41.0 25 35.928124604636125 40.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.659035831635908 1101 2 -0.7967033435529736 1101 3 -0.29717870715323613 1101 4 -0.8055938560252685 1101 5 -0.5836483018615013 1101 6 -0.9780033700024902 1101 7 -0.6744705273999188 1101 8 -0.76189442915706 1101 9 -0.8416953521809347 1101 10 -0.6675897459335616 1101 11 -0.7659581656793364 1101 12 -0.7721687709133604 1101 13 -0.9122667998647387 1101 14 -0.8834759191728025 1101 15 -0.5744355398652203 1101 16 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0.49914629202000427 1103 2 0.5810688338663788 1103 3 0.1717393035994519 1103 4 0.6308980794586034 1103 5 0.3381426927621547 1103 6 0.30257458698675777 1103 7 0.42028753711620936 1103 8 0.4602157858967004 1103 9 0.9745008843782088 1103 10 0.7397523239589106 1103 11 0.7243812160410883 1103 12 0.5435234333877119 1103 13 0.4373635764793491 1103 14 0.49505230663365296 1103 15 0.43682118056488406 1103 16 0.3983377969631974 1103 17 0.5123303794591436 1103 18 0.8286741167400891 1103 19 0.7376367470058725 1103 20 0.6505383524213784 1103 21 0.5830066502642808 1103 22 0.46368348979675034 1103 23 0.5384732455280457 1103 24 0.63700226261426 1103 25 0.34128324519419095 1104 1 -0.3213680813650086 1104 2 -0.5678127875806496 1104 3 -0.566350166684785 1104 4 -0.21123693346129357 1104 5 -0.9765173524574848 1104 6 -0.805064301902128 1104 7 -0.8043571657261737 1104 8 -0.9851169014417991 1104 9 -1.3719945936579663 1104 10 -1.2967261967129247 1104 11 -1.0117904570855245 1104 12 -1.2701474322453663 1104 13 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15 -0.0778568497381471 2215 16 0.10690787272076818 2215 17 0.2540443946106592 2215 18 0.3272536621946358 2215 19 0.5082333379149588 2215 20 0.30086979181531603 2215 21 0.5382623320824607 2215 22 0.40165697464523475 2215 23 0.6286721091644054 2215 24 0.6481291565536509 2215 25 0.7650521845881357 2216 1 0.14155617029715728 2216 2 0.004553150345781631 2216 3 -0.13543532186871232 2216 4 -0.30039873702079944 2216 5 0.09156614781833383 2216 6 -0.47048252911810806 2216 7 -0.034534560262066805 2216 8 -0.019536086762471427 2216 9 -0.28146821674538813 2216 10 0.11267484268550021 2216 11 -0.3784687371424269 2216 12 -0.026759806312661283 2216 13 -0.3495629216479941 2216 14 -0.0992243787596081 2216 15 -0.04755381943511594 2216 16 -0.02619989030272052 2216 17 -0.28381311305022194 2216 18 -0.14142185704267973 2216 19 -0.16039110879937368 2216 20 -0.1949380052565246 2216 21 -0.3340989208967713 2216 22 -0.17050424428564526 2216 23 -0.24573204473412602 2216 24 -0.15685892652432187 2216 25 0.14260581073726541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 292.0 3 49.0 4 19.0 5 30.0 6 26.0 7 81.0 8 72.0 9 137.0 10 239.0 11 334.0 12 525.0 13 840.0 14 1585.0 15 2737.0 16 3686.0 17 4069.0 18 4147.0 19 3833.0 20 3533.0 21 3217.0 22 2815.0 23 2560.0 24 2406.0 25 2235.0 26 2013.0 27 1864.0 28 1666.0 29 1602.0 30 1796.0 31 2268.0 32 2722.0 33 3394.0 34 4553.0 35 6874.0 36 12536.0 37 30347.0 38 173446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16688867878771 25.042264312752398 10.247470291966442 21.54337671649345 2 11.68388509778238 31.60832118536046 29.29738941823534 27.41040429862182 3 21.267170680348045 37.87462102138478 23.68418272667411 17.174025571593067 4 13.418657465274721 29.060789042832248 23.60473918631058 33.91581430558245 5 9.941083873603251 46.06586069311847 30.362758528399823 13.630296904878453 6 28.64350269158865 35.915545024697835 15.826682110881482 19.61427017283203 7 17.933622990415966 42.77782081165013 19.912750779891134 19.37580541804277 8 25.75355967404122 39.09674921238362 18.996075118362462 16.1536159952127 9 24.712273401000573 25.903667479940996 17.141780824329224 32.24227829472921 10 17.8206107810998 39.176118057585114 25.074560646767395 17.92871051454769 11 22.887706934847422 35.069186552006954 14.480775255467515 27.562331257678107 12 22.703174614350186 40.950694556898746 22.587356459414362 13.758774369336704 13 28.228938870518466 28.58108750541429 19.0758915523064 24.114082071760848 14 20.452697064757018 37.44626466829325 20.92271618238854 21.178322084561195 15 34.129356386667936 27.156673250315983 18.652851565112996 20.06111879790309 16 15.262209204078184 34.54451360333465 30.597997521545654 19.595279671041514 17 24.993222809383152 36.986209737326654 22.126031101143848 15.894536352146346 18 24.126167105578165 28.906194989367545 27.066991508118686 19.9006463969356 19 21.93333544542187 38.11686185884912 26.920680514927188 13.029122180801819 20 23.548714193166774 30.316100047883275 24.509900571782666 21.625285187167282 21 18.09850079924512 34.89285890330894 28.179199909864867 18.82944038758107 22 22.256835909999715 33.701177100053506 17.96274393849793 26.07924305144885 23 16.79863118352632 33.35750824704884 26.20325938860939 23.640601180815445 24 21.360617989000698 40.52876930335401 20.424409720510674 17.68620298713462 25 21.466096388355286 29.79774931103163 18.994300754592828 29.74185354602026 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12410.0 1 12410.0 2 9192.5 3 5975.0 4 5975.0 5 5975.0 6 5489.0 7 5003.0 8 5003.0 9 5003.0 10 4515.5 11 4028.0 12 4028.0 13 4028.0 14 3632.5 15 3237.0 16 3237.0 17 3237.0 18 3627.0 19 4017.0 20 4017.0 21 4017.0 22 4693.5 23 5370.0 24 5370.0 25 5370.0 26 6319.5 27 7269.0 28 7269.0 29 7269.0 30 7903.5 31 8538.0 32 8538.0 33 8538.0 34 8733.0 35 8928.0 36 8928.0 37 8928.0 38 8884.0 39 8840.0 40 8840.0 41 8840.0 42 12803.0 43 16766.0 44 16766.0 45 16766.0 46 54567.5 47 92369.0 48 92369.0 49 92369.0 50 76749.0 51 61129.0 52 61129.0 53 61129.0 54 41390.0 55 21651.0 56 21651.0 57 21651.0 58 14523.0 59 7395.0 60 7395.0 61 7395.0 62 6571.0 63 5747.0 64 5747.0 65 5747.0 66 4345.0 67 2943.0 68 2943.0 69 2943.0 70 2350.5 71 1758.0 72 1758.0 73 1758.0 74 1265.5 75 773.0 76 773.0 77 773.0 78 537.0 79 301.0 80 301.0 81 301.0 82 191.5 83 82.0 84 82.0 85 82.0 86 48.5 87 15.0 88 15.0 89 15.0 90 9.5 91 4.0 92 4.0 93 4.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010191602119853241 2 0.19328900572135457 3 0.19715478583578164 4 0.18731461827178544 5 0.20558921517634987 6 0.18098879626635928 7 0.18766605282764243 8 0.16271419936179485 9 0.17958305804293126 10 0.19364044027721158 11 0.16201133025008083 12 0.169391455923078 13 0.2027777387294938 14 0.1816916653780733 15 0.17958305804293126 16 0.17571727792850414 17 0.17817731981950322 18 0.17993449259878824 19 0.16482280669693689 20 0.18415170726907235 21 0.1862603146042144 22 0.16165989569422382 23 0.17817731981950322 24 0.1862603146042144 25 0.6603455304553186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 284548.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.41186724208218 #Duplication Level Percentage of deduplicated Percentage of total 1 78.98916208832448 18.492837763751634 2 11.007535500915669 5.1541391961988845 3 4.054459755621604 2.8476742061093385 4 1.829835780119487 1.7135948943587724 5 1.0012308985559457 1.1720342437831228 6 0.5659131165750998 0.7949449653485529 7 0.4143024407817707 0.67897156191574 8 0.25068299858897 0.4695165666249631 9 0.19814464559128162 0.4175042523581258 >10 1.2894412921432648 6.121989963029085 >50 0.18913807079167794 3.1042214318849544 >100 0.13810081359392357 7.501019160211985 >500 0.01501095799933952 2.3753461630375194 >1k 0.046533969797952504 25.200317696838493 >5k 0.006004383199735807 8.965798389023997 >10k+ 0.004503287399801856 14.990089545524832 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 15700 5.51752252695503 No Hit CCCATGTACTCTGCGTTGATACCAC 15208 5.344616725473382 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 11746 4.127950293096419 No Hit GAGTACATGGGAAGCAGTGGTATCA 7998 2.8107735777443525 No Hit CATGTACTCTGCGTTGATACCACTG 6670 2.3440684875662456 No Hit GCGTTGATACCACTGCTTCCCATGT 5429 1.9079382037476982 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5415 1.9030181199657 No Hit GGTATCAACGCAGAGTACATGGGAA 4982 1.7508469572796153 No Hit TATCAACGCAGAGTACATGGGAAGC 4705 1.653499585307224 No Hit ACTCTGCGTTGATACCACTGCTTCC 4660 1.6376850302936588 No Hit GTATCAACGCAGAGTACATGGGAAG 4498 1.5807526322448233 No Hit GTGGTATCAACGCAGAGTACATGGG 3456 1.2145578250418207 No Hit GTACTCTGCGTTGATACCACTGCTT 3201 1.1249420132982837 No Hit GCTTCCCATGTACTCTGCGTTGATA 3080 1.0824184320395855 No Hit GATACCACTGCTTCCCATGTACTCT 2676 0.9404388714733543 No Hit ATACCACTGCTTCCCATGTACTCTG 2508 0.8813978660893769 No Hit CAGTGGTATCAACGCAGAGTACATG 2346 0.8244654680405414 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2334 0.8202482533702574 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2232 0.7844019286728424 No Hit CATGGGAAGCAGTGGTATCAACGCA 2194 0.7710474155502762 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2077 0.7299295725150062 No Hit GTTGATACCACTGCTTCCCATGTAC 2048 0.719737970395153 No Hit TACCACTGCTTCCCATGTACTCTGC 1970 0.6923260750383063 No Hit GCAGTGGTATCAACGCAGAGTACAT 1930 0.678268692804026 No Hit GTATCAACGCAGAGTACTTTTTTTT 1926 0.676862954580598 No Hit ACATGGGAAGCAGTGGTATCAACGC 1867 0.6561283157850345 No Hit CTTCCCATGTACTCTGCGTTGATAC 1782 0.6262563785371887 No Hit CCACTGCTTCCCATGTACTCTGCGT 1740 0.6114961271911945 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1634 0.5742440642703516 No Hit GGTATCAACGCAGAGTACTTTTTTT 1559 0.5478864725810759 No Hit CCATGTACTCTGCGTTGATACCACT 1500 0.5271518337855124 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1496 0.5257460955620844 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 1376 0.48357394885924343 No Hit TGATACCACTGCTTCCCATGTACTC 1343 0.4719766085159622 No Hit TTCCCATGTACTCTGCGTTGATACC 1221 0.4291015927014072 No Hit TATCAACGCAGAGTACTTTTTTTTT 1216 0.4273444199221221 No Hit ATCAACGCAGAGTACATGGGAAGCA 1108 0.38938948788956523 No Hit GTACATGGTAAGCAGTGGTATCAAC 1042 0.3661948072030026 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 792 0.27833616823875057 No Hit GGAAGCAGTGGTATCAACGCAGAGT 783 0.2751732572360375 No Hit ACCATGTACTCTGCGTTGATACCAC 774 0.27201034623332443 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 703 0.24705849276747682 No Hit AAAAACAAAAAAAAAAAAAAAAAAA 652 0.2291353304187694 No Hit CTGCGTTGATACCACTGCTTCCCAT 634 0.22280950841334327 No Hit CTCTGCGTTGATACCACTGCTTCCC 619 0.21753799007548816 No Hit TCAACGCAGAGTACATGGGAAGCAG 616 0.21648368640791713 No Hit TGGGAAGCAGTGGTATCAACGCAGA 597 0.20980642984663395 No Hit AAAAAGTACTCTGCGTTGATACCAC 589 0.2069949533997779 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 498 0.17501440881679012 No Hit AGTGGTATCAACGCAGAGTACATGG 497 0.17466297426093313 No Hit CCCCATGTACTCTGCGTTGATACCA 494 0.1736086705933621 No Hit AACGCAGAGTACATGGGAAGCAGTG 494 0.1736086705933621 No Hit AAAGTACTCTGCGTTGATACCACTG 484 0.17009432503479202 No Hit ATGTACTCTGCGTTGATACCACTGC 478 0.16798571769964998 No Hit ACACAAAAAAAAAAAAAAAAAAAAA 469 0.16482280669693689 No Hit GGTAAGCAGTGGTATCAACGCAGAG 468 0.16447137214107987 No Hit TCCATGTACTCTGCGTTGATACCAC 457 0.1606055920266528 No Hit GAGTACATGGTAAGCAGTGGTATCA 442 0.15533407368879767 No Hit TCCCATGTACTCTGCGTTGATACCA 433 0.1521711626860846 No Hit ACTGCTTCCCATGTACTCTGCGTTG 424 0.1490082516833715 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 399 0.14022238778694632 No Hit GCTTACCATGTACTCTGCGTTGATA 389 0.13670804222837624 No Hit TTGATACCACTGCTTCCCATGTACT 374 0.1314365238905211 No Hit GTACATGGAAAGCAGTGGTATCAAC 370 0.13003078566709306 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 363 0.127570743776094 No Hit GTACATGGGTAAGCAGTGGTATCAA 355 0.12475926732923796 No Hit GTGGTATCAACGCAGAGTACTTTTT 344 0.12089348721481086 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 336 0.1180820107679548 No Hit CGTTGATACCACTGCTTCCCATGTA 324 0.11386479609767068 No Hit CAACGCAGAGTACATGGGAAGCAGT 322 0.11316192698595667 No Hit ACTCTGCGTTGATACCACTGCTTAC 320 0.11245905787424265 No Hit CGCAGAGTACATGGGAAGCAGTGGT 319 0.11210762331838565 No Hit GCGTTGATACCACTGCTTACCATGT 302 0.1061332358688165 No Hit ACGCAGAGTACATGGTAAGCAGTGG 297 0.10437606308953146 No Hit GGTATCAACGCAGAGTACATGGGGG 292 0.10261889031024643 No Hit CAAAAAAAAAAAAAAAAAAAAAAAA 288 0.1012131520868184 No Hit GAGTACATGGAAAGCAGTGGTATCA 285 0.10015884841924738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.514345558570083E-4 0.0 9 0.0 0.0 0.0 3.514345558570083E-4 0.0 10 0.0 0.0 0.0 7.028691117140166E-4 0.0 11 0.0 0.0 0.0 7.028691117140166E-4 0.0 12 0.0 0.0 0.0 7.028691117140166E-4 0.0 13 0.0 0.0 0.0 0.0010543036675710249 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 50 2.1579399E-7 18.999823 1 GAACAAA 250 0.0 14.439867 1 GGAGTAT 40 0.005259237 14.252377 5 GGAAACA 55 1.9514457E-4 13.818054 1 AGAACAA 75 2.0626414E-4 11.399895 2 TACAAAA 110 4.913727E-7 11.227169 2 GAGAACA 85 5.2877127E-5 11.176368 1 ACGCAAA 75 0.0026364007 10.13324 1 CGCAAAA 75 0.0026364007 10.13324 2 TCCTGAA 85 6.558238E-4 10.05873 2 AAAGTAC 160 6.6440407E-7 8.906167 1 ATCCTGA 130 4.32824E-5 8.76915 1 GAAACAA 130 4.32824E-5 8.76915 2 CAAAAAC 165 1.0323474E-6 8.637803 4 AGTACTC 170 1.4341067E-5 7.8234572 3 GAAAACA 135 6.2257936E-4 7.740669 1 AAGTACT 175 2.1095755E-5 7.5999303 2 GAAGCAG 4515 0.0 7.1946797 9 CACAAAA 480 0.0 7.1249337 2 CAGTGGT 4910 0.0 7.09825 13 >>END_MODULE