FastQCFastQC Report
Wed 25 May 2016
SRR1294835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284548
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC164305.774069752730647No Hit
CCCATGTACTCTGCGTTGATACCAC160005.6229528937121325No Hit
GAGTACATGGGAAGCAGTGGTATCA85322.998439630571995No Hit
GTATCAACGCAGAGTACTTTTTTTT71602.5162714199361793No Hit
CATGTACTCTGCGTTGATACCACTG68042.3911607180510845No Hit
GGTATCAACGCAGAGTACTTTTTTT59662.0966585602429118No Hit
ACGCAGAGTACATGGGAAGCAGTGG57962.0369146857472202No Hit
GCGTTGATACCACTGCTTCCCATGT56291.9782251149190997No Hit
TATCAACGCAGAGTACTTTTTTTTT50261.7663100777373235No Hit
GGTATCAACGCAGAGTACATGGGAA49821.7508469572796153No Hit
TATCAACGCAGAGTACATGGGAAGC49421.736789575045335No Hit
ACTCTGCGTTGATACCACTGCTTCC48031.687940171781211No Hit
GTATCAACGCAGAGTACATGGGAAG44261.5554493442231188No Hit
GTGGTATCAACGCAGAGTACATGGG35311.2409154167310965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33041.1611397725515555No Hit
GCTTCCCATGTACTCTGCGTTGATA31651.1122903692874313No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30061.0564122749061668No Hit
GTACTCTGCGTTGATACCACTGCTT29581.0395434162250305No Hit
GATACCACTGCTTCCCATGTACTCT28210.9913968820726204No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26760.9404388714733543No Hit
CAGTGGTATCAACGCAGAGTACATG25630.9007267666615124No Hit
GGGAAGCAGTGGTATCAACGCAGAG25130.8831550388686618No Hit
ATACCACTGCTTCCCATGTACTCTG24460.8596089236262424No Hit
CATGGGAAGCAGTGGTATCAACGCA24310.8543374052883872No Hit
CTGCTTCCCATGTACTCTGCGTTGA23150.8135709968089742No Hit
ACATGGGAAGCAGTGGTATCAACGC22110.7770218029998454No Hit
GCAGAGTACATGGGAAGCAGTGGTA21870.7685873736592771No Hit
GCAGTGGTATCAACGCAGAGTACAT20880.7337953526294333No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20160.7084920646077287No Hit
GTTGATACCACTGCTTCCCATGTAC20120.7070863263843008No Hit
TACCACTGCTTCCCATGTACTCTGC19350.6800258655833111No Hit
CTTCCCATGTACTCTGCGTTGATAC18160.6382051534363271No Hit
CCACTGCTTCCCATGTACTCTGCGT18110.6364479806570421No Hit
AAGCAGTGGTATCAACGCAGAGTAC16730.5879500119487748No Hit
ATGGGAAGCAGTGGTATCAACGCAG16190.5689725459324965No Hit
CCATGTACTCTGCGTTGATACCACT15860.5573752055892152No Hit
TGATACCACTGCTTCCCATGTACTC14040.4934141164232396No Hit
GTGGTATCAACGCAGAGTACTTTTT13190.463542179175394No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12610.44315897493568746No Hit
TTCCCATGTACTCTGCGTTGATACC12560.44140180215640246No Hit
ATCAACGCAGAGTACATGGGAAGCA10880.382360796772425No Hit
GTACATGGTAAGCAGTGGTATCAAC10590.3721691946525718No Hit
GGAAGCAGTGGTATCAACGCAGAGT9130.3208597494974486No Hit
ATCAACGCAGAGTACTTTTTTTTTT8550.30047654525774214No Hit
ACCATGTACTCTGCGTTGATACCAC8480.298016503366743No Hit
CTGCGTTGATACCACTGCTTCCCAT6770.23792119431519462No Hit
TCAACGCAGAGTACATGGGAAGCAG6510.2287838958629124No Hit
CTCTGCGTTGATACCACTGCTTCCC6490.22808102675119837No Hit
TGGGAAGCAGTGGTATCAACGCAGA6350.22316094296920028No Hit
AAAAAGTACTCTGCGTTGATACCAC6270.22034946652234422No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA5870.20629208428806386No Hit
GGTAAGCAGTGGTATCAACGCAGAG5320.18696318371592843No Hit
AGTGGTATCAACGCAGAGTACATGG5270.18520601093664338No Hit
TCCATGTACTCTGCGTTGATACCAC5260.18485457638078637No Hit
CCCCATGTACTCTGCGTTGATACCA5240.18415170726907235No Hit
AAAGTACTCTGCGTTGATACCACTG4760.16728284858793596No Hit
AACGCAGAGTACATGGGAAGCAGTG4730.16622854492036493No Hit
TCCCATGTACTCTGCGTTGATACCA4460.1567398119122257No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4250.14935968623922852No Hit
ACTGCTTCCCATGTACTCTGCGTTG4190.14725107890408648No Hit
ATGTACTCTGCGTTGATACCACTGC4170.14654820979237246No Hit
GTACATGGGTAAGCAGTGGTATCAA4060.1426824296779454No Hit
GAGTACATGGTAAGCAGTGGTATCA4040.14197956056623137No Hit
GCTTACCATGTACTCTGCGTTGATA3990.14022238778694632No Hit
GTACATGGAAAGCAGTGGTATCAAC3920.13776234589594727No Hit
AACGCAGAGTACTTTTTTTTTTTTT3910.13741091134009026No Hit
CAACGCAGAGTACTTTTTTTTTTTT3900.13705947678423325No Hit
TTGATACCACTGCTTCCCATGTACT3750.1317879584463781No Hit
CAACGCAGAGTACATGGGAAGCAGT3710.13038222022295007No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3520.12370496366166692No Hit
ACTCTGCGTTGATACCACTGCTTAC3340.11737914165624078No Hit
ACGCAGAGTACATGGTAAGCAGTGG3330.11702770710038378No Hit
CGCAGAGTACATGGGAAGCAGTGGT3290.11562196887695572No Hit
TCAACGCAGAGTACTTTTTTTTTTT3270.11491909976524171No Hit
CGTTGATACCACTGCTTCCCATGTA3020.1061332358688165No Hit
GAGTACATGGAAAGCAGTGGTATCA2910.10226745575438942No Hit
GGTATCAACGCAGAGTACATGGGGG2850.10015884841924738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATTC350.002182078516.2658319
GAACAAA1058.185452E-1114.527451
GGCGCGC400.005307934214.2326018
GGTCAGT602.5833328E-514.23260117
TCAGTCA655.4845197E-513.13778719
GTCAGTC655.4845197E-513.13778718
AAAAGTA1251.3151293E-912.2009042
CGGTCAG701.0973155E-412.19937316
AAAAAGT1302.4319888E-911.733711
TCGGTCA752.0860211E-411.38608115
GAAAAAA3000.010.8047921
TATCGGT856.6318194E-410.04654113
ATCGGTC856.6318194E-410.04654114
CTATCGG900.00112008419.488412
GTACTGG1151.0601418E-49.1191341
ACTGGTT950.00175929519.0302743
GTTCACT950.0018342268.9890117
AACAAAA3100.08.60950852
TACTGGT1000.0028028298.578762
TTCACTA1000.00292157668.5395618
Produced by FastQC (version 0.11.5)