##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294835_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284548
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980674613773424	33.0	31.0	34.0	30.0	34.0
2	32.08930654933438	33.0	31.0	34.0	30.0	34.0
3	32.21144060053137	34.0	31.0	34.0	30.0	34.0
4	35.54583760912043	37.0	35.0	37.0	35.0	37.0
5	35.69762570814063	37.0	35.0	37.0	35.0	37.0
6	35.51442638851793	37.0	37.0	37.0	35.0	37.0
7	35.647001560369425	37.0	37.0	37.0	35.0	37.0
8	35.60670607419486	37.0	37.0	37.0	35.0	37.0
9	37.31920800708492	39.0	39.0	39.0	35.0	39.0
10	37.415272642928436	39.0	39.0	39.0	35.0	39.0
11	37.411132743860435	39.0	39.0	39.0	35.0	39.0
12	37.48584070174452	39.0	39.0	39.0	35.0	39.0
13	37.30308067531664	39.0	39.0	39.0	35.0	39.0
14	38.71182717854281	41.0	39.0	41.0	35.0	41.0
15	38.61531622081336	41.0	39.0	41.0	35.0	41.0
16	38.483890239959514	41.0	39.0	41.0	34.0	41.0
17	38.69901036029071	41.0	39.0	41.0	36.0	41.0
18	38.67716518829864	41.0	39.0	41.0	35.0	41.0
19	38.621786130986685	41.0	39.0	41.0	34.0	41.0
20	38.709824001574425	41.0	39.0	41.0	35.0	41.0
21	38.65032613126783	41.0	39.0	41.0	34.0	41.0
22	38.74397992605817	41.0	39.0	41.0	35.0	41.0
23	38.670083781998116	41.0	39.0	41.0	35.0	41.0
24	38.660187384905186	41.0	39.0	41.0	34.0	41.0
25	37.85668850246707	41.0	38.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.045427144878807724
1101	2	-0.03995508195951203
1101	3	0.10275641925377954
1101	4	-0.024842402263317354
1101	5	-0.3217498243679344
1101	6	-0.003894544133565603
1101	7	0.1702850390996602
1101	8	0.020871201016504415
1101	9	0.14748649943006598
1101	10	0.2276098947000662
1101	11	0.16849869520115135
1101	12	0.19168259299205914
1101	13	0.22858328442647036
1101	14	0.020681358256929627
1101	15	0.18403260441310465
1101	16	0.29934683600974665
1101	17	0.41923577402302925
1101	18	0.36497743270242466
1101	19	0.23579056922276465
1101	20	0.3346371744346328
1101	21	0.35835710150616507
1101	22	0.24912590278199787
1101	23	0.21095422069801373
1101	24	0.19187101367845827
1101	25	0.3948540854082623
1102	1	-0.1900107252839156
1102	2	-0.21927197000505316
1102	3	-0.4122082176273629
1102	4	-0.27350550386759664
1102	5	-0.275605266838177
1102	6	0.10302857702104973
1102	7	0.09901254183095887
1102	8	0.20474504380874947
1102	9	0.20373685881135373
1102	10	0.18937172553210502
1102	11	0.3311834171837873
1102	12	0.18261180925334486
1102	13	0.20173031449949264
1102	14	0.2813909206742693
1102	15	0.4416802382466969
1102	16	0.35650283623974843
1102	17	0.17520150342329543
1102	18	0.2765983860691108
1102	19	-0.04684125183410259
1102	20	0.16594359729703
1102	21	0.14175081089607744
1102	22	0.024469471294452205
1102	23	-0.06664626231044224
1102	24	-0.08289754477131339
1102	25	0.0029416019398027515
1103	1	0.028962091115367627
1103	2	0.08924518685769556
1103	3	0.0022859891462516657
1103	4	0.08453259773668265
1103	5	0.18228243369658514
1103	6	0.15218744511374638
1103	7	0.2668072165190125
1103	8	0.2490298031670406
1103	9	0.34599120373113834
1103	10	0.19165559362480167
1103	11	0.13741670595383937
1103	12	0.27669603385227504
1103	13	0.3138487414157183
1103	14	0.34628619696660223
1103	15	0.437056797961489
1103	16	0.30522721235383443
1103	17	0.36295217187249307
1103	18	0.38757487893898457
1103	19	0.4018691982550209
1103	20	0.617063249703449
1103	21	0.29568909075347705
1103	22	0.35556071460995753
1103	23	0.37258056478403034
1103	24	0.4351505835709304
1103	25	0.28430301013944614
1104	1	-0.3793873920887627
1104	2	-0.3667867898864934
1104	3	-0.30415780930335856
1104	4	-0.2819483454157705
1104	5	-0.3257925017039298
1104	6	-0.23536907943405794
1104	7	-0.12537220854558484
1104	8	0.004763007301406219
1104	9	-0.21741722650141782
1104	10	-0.0973463443596998
1104	11	-0.27767534830972807
1104	12	-0.28233174392550353
1104	13	0.07400297268186762
1104	14	-0.26926612861478816
1104	15	0.08248638452479895
1104	16	0.050826372560550226
1104	17	-0.1596172725719498
1104	18	-0.018493435014498516
1104	19	0.03480282228603215
1104	20	-0.1193304841466798
1104	21	-0.18448694283825517
1104	22	-0.09496011872337817
1104	23	-0.09568493909193876
1104	24	0.062088568067053984
1104	25	-0.0504030622119771
1105	1	-0.005964379472864323
1105	2	-0.07221314647564014
1105	3	0.08868304796978066
1105	4	-0.002783578733904335
1105	5	0.18473341408873978
1105	6	0.2137683274666884
1105	7	0.2859555008327419
1105	8	0.2876327443435116
1105	9	0.27169586059387996
1105	10	0.2118761818600916
1105	11	0.1616201373263877
1105	12	-0.02171082889282161
1105	13	0.12864653553336325
1105	14	0.01601658912346693
1105	15	0.302865621490902
1105	16	0.38457770254991175
1105	17	0.15002325030386743
1105	18	0.29397806521349423
1105	19	0.3419733649216923
1105	20	0.05364413205639096
1105	21	0.009843502518180003
1105	22	0.20651046951191887
1105	23	0.2261344863526631
1105	24	0.15328783847289174
1105	25	0.21996477484532306
1106	1	-0.012931573129989715
1106	2	4.2607870595645636E-4
1106	3	0.09492622320598088
1106	4	0.09501231928631881
1106	5	-0.09223330480220682
1106	6	-0.17997306739634666
1106	7	-0.21215136903384746
1106	8	-0.001499857849907471
1106	9	0.05855551971176709
1106	10	0.0976533740929213
1106	11	0.12080804977950521
1106	12	-0.27452975307266314
1106	13	0.13564933665381318
1106	14	0.0070257046340813645
1106	15	-0.02811168468580405
1106	16	0.04684091291880321
1106	17	-0.29386532207423954
1106	18	-0.1437444814322788
1106	19	-0.21200161490074976
1106	20	-0.27764770671301875
1106	21	-0.09253982369366298
1106	22	-0.044919763598272766
1106	23	-0.24513029074461912
1106	24	-0.07725861739758955
1106	25	-0.16638807137790934
1107	1	-0.023708096678056734
1107	2	-0.07221314647564014
1107	3	-0.06652269629975649
1107	4	-0.1187391158783413
1107	5	-0.3053936226414464
1107	6	-0.3801629069989332
1107	7	-0.24452641024154786
1107	8	-0.3787577555184072
1107	9	-0.23745216481346176
1107	10	-0.2327868946381102
1107	11	-0.1230733175203369
1107	12	-0.17591546849514117
1107	13	-0.03705354731668109
1107	14	-0.12803892040428622
1107	15	-0.5771744226957836
1107	16	-0.41154215384334947
1107	17	-0.15179254643736328
1107	18	-0.40368832528636744
1107	19	-0.15157123601727562
1107	20	-0.11012278316039925
1107	21	-0.4216671299038026
1107	22	-0.2710164567515463
1107	23	-0.06888070282160186
1107	24	-0.12412171689855
1107	25	-0.23785074572909792
1108	1	0.40680727546450157
1108	2	0.2934753411090085
1108	3	0.34613004482721976
1108	4	0.36680545326715475
1108	5	0.19441561654083017
1108	6	0.371090285595308
1108	7	0.3129826773918509
1108	8	0.4165664848826154
1108	9	0.48120212359570047
1108	10	0.5070948336548966
1108	11	0.3806094260441313
1108	12	0.4683579977348913
1108	13	0.5242336642900582
1108	14	0.5848819456498262
1108	15	0.45217624867631656
1108	16	0.39271075260966626
1108	17	0.3818926346264462
1108	18	0.4599918748005365
1108	19	0.46716147628361426
1108	20	0.2979675950759102
1108	21	0.2581726310093089
1108	22	0.12324906111108902
1108	23	0.43284674236115706
1108	24	0.3033785745415827
1108	25	0.3939013923817285
1109	1	0.17819065726466476
1109	2	0.10073585574165378
1109	3	-0.03376814980422438
1109	4	-0.2831572581391484
1109	5	-0.15613220044975407
1109	6	-0.4316655667118283
1109	7	-0.26521075280840734
1109	8	-0.5237667600258646
1109	9	-0.5598603760914784
1109	10	-0.3275840589990011
1109	11	-0.4161101787468269
1109	12	-0.2517823475224503
1109	13	-0.15766710300409414
1109	14	-0.3208655029594851
1109	15	-0.4473898021863292
1109	16	-0.2885343360571042
1109	17	-0.20962959087695054
1109	18	-0.12753621677424576
1109	19	-0.03321140965332603
1109	20	-0.005961972026042872
1109	21	-0.1326068257284163
1109	22	-0.1518706878290672
1109	23	-0.2125312238338566
1109	24	-0.23086937208316982
1109	25	-0.4382348686484363
1110	1	8.853802985946402E-4
1110	2	0.06465219568771374
1110	3	-0.0057852029067291255
1110	4	0.20804942996626608
1110	5	-0.09227606519966258
1110	6	0.17726332811594858
1110	7	-0.06606047111895208
1110	8	0.0851915029462873
1110	9	-0.05334309538586268
1110	10	0.09583605720095534
1110	11	-0.03418235585631635
1110	12	0.00537980868671184
1110	13	-0.005946374478327243
1110	14	0.12767416606152437
1110	15	0.13014454630586414
1110	16	-0.05331997307663272
1110	17	0.09718920277757093
1110	18	0.1990228645901979
1110	19	0.3814325260695952
1110	20	0.43904862497938524
1110	21	0.5052986382148461
1110	22	0.23837321460995753
1110	23	0.15617603939772096
1110	24	0.09602254825084344
1110	25	0.33900775627630964
1111	1	0.13820354410682256
1111	2	0.3273595030970071
1111	3	0.26804453615480384
1111	4	0.15037127295035901
1111	5	0.26241063882478954
1111	6	0.13175475280605298
1111	7	-0.19399673647243532
1111	8	0.08690373479097246
1111	9	-0.030277492850061094
1111	10	0.22664490986411323
1111	11	-0.0782617128495815
1111	12	-0.0985710601648222
1111	13	-0.30360585260137896
1111	14	-0.1860321791017725
1111	15	0.11627688343157416
1111	16	-0.1064982149965985
1111	17	-0.0314121443668256
1111	18	-0.03797266379605446
1111	19	0.07167421962254394
1111	20	0.12868183944704015
1111	21	0.023920928360311677
1111	22	-0.13342165718491117
1111	23	0.004792103245570445
1111	24	-0.14711437369402347
1111	25	-0.1791051095186731
1112	1	-0.13064812393839276
1112	2	-0.10850346905628427
1112	3	-0.17748551623009234
1112	4	-0.010729499037509527
1112	5	0.26494372401916877
1112	6	-0.3439483075744292
1112	7	-0.2907364662766838
1112	8	-0.14109116457489534
1112	9	-0.555470489817246
1112	10	-0.40679870745047
1112	11	-0.3933292617880966
1112	12	-0.25455396614772496
1112	13	-0.3352608015950338
1112	14	-0.33146917937747844
1112	15	-0.5229566428987269
1112	16	-0.24433595968918098
1112	17	-0.3260599249016991
1112	18	-0.296984932888968
1112	19	-0.23127932325035516
1112	20	-0.3219488470707432
1112	21	-0.3304869845153391
1112	22	-0.08420743055133073
1112	23	-0.19831997285037772
1112	24	-0.419688126106486
1112	25	-0.2072964522261458
1113	1	0.139403124253775
1113	2	-0.0039870256179384
1113	3	0.17473969284996116
1113	4	0.10283790962751738
1113	5	0.03827658574336823
1113	6	-0.07262371473031237
1113	7	0.15129491145079044
1113	8	0.046605339229415677
1113	9	0.05421793375062833
1113	10	0.13067849810823162
1113	11	0.1558438502033539
1113	12	0.09583589740003617
1113	13	0.05015783010967567
1113	14	0.10962684024146085
1113	15	0.14581654455018622
1113	16	0.043074434576055864
1113	17	0.08599676251576938
1113	18	0.24257914307153783
1113	19	0.08929305303085044
1113	20	0.19926963371904094
1113	21	0.03914060147472043
1113	22	0.2754102516469956
1113	23	0.18465415152866882
1113	24	0.3842246576450066
1113	25	0.15205398479831445
1114	1	0.34864251182734307
1114	2	0.38409753313600703
1114	3	0.3383499050038594
1114	4	0.6576818695813458
1114	5	0.24708825893930708
1114	6	0.09849610207167103
1114	7	-0.23242923170104746
1114	8	0.17227009442917307
1114	9	0.3012551600418192
1114	10	0.2858101243691351
1114	11	0.13034753119655562
1114	12	-0.038534548671997015
1114	13	0.15367984982995608
1114	14	0.3277384623387718
1114	15	0.1388974128482232
1114	16	0.050625675137006
1114	17	-0.053036905797412714
1114	18	0.07013454722377332
1114	19	0.27490216751068886
1114	20	0.14136543416946523
1114	21	0.17628172829392952
1114	22	0.10245088451286932
1114	23	0.11519787869988818
1114	24	-0.07431543584654321
1114	25	0.017899288456597162
1115	1	0.2970754051063409
1115	2	0.30496286899824554
1115	3	0.2589147770250406
1115	4	0.2275753927076707
1115	5	0.13900390371592408
1115	6	0.1561062877958861
1115	7	0.0792719570090199
1115	8	0.2582778689310672
1115	9	0.16146811378916226
1115	10	0.16701423321216424
1115	11	0.2868666575979404
1115	12	0.14728355803022453
1115	13	0.11520169918489387
1115	14	0.2230793949034222
1115	15	0.07013626282287078
1115	16	-0.023119418851841544
1115	17	0.28289293589570264
1115	18	-0.06615960687661726
1115	19	0.16313713023438936
1115	20	0.15354173132820392
1115	21	-0.04547185379842489
1115	22	-0.028551350186944546
1115	23	0.0056600296454121235
1115	24	0.05289719865797338
1115	25	-0.001097537894082734
1116	1	0.21929589349185363
1116	2	0.26362175033486324
1116	3	0.24714605793924704
1116	4	0.07553283225826135
1116	5	0.1628171428898284
1116	6	0.1398848341068657
1116	7	0.05527364084672115
1116	8	0.07882576939593378
1116	9	0.12784216526959824
1116	10	0.2112186017548794
1116	11	0.02768992358986111
1116	12	0.24028655918060338
1116	13	-0.03401027203018714
1116	14	0.06886671604102901
1116	15	0.19153225136987118
1116	16	-0.06219969508831724
1116	17	0.10283842890814299
1116	18	0.18424955842616697
1116	19	-0.0028515256349095353
1116	20	0.11070185195485749
1116	21	0.24416274616310574
1116	22	0.2196114885311573
1116	23	0.39347252851136005
1116	24	0.2501130601187711
1116	25	0.0546868437854755
1201	1	-0.26868861810448763
1201	2	-0.07221314647564014
1201	3	-0.28339663496722167
1201	4	-0.02218282779523406
1201	5	-0.5225048003459705
1201	6	0.004026984120834243
1201	7	0.25456209240554273
1201	8	0.0728329946564017
1201	9	0.23279306543326328
1201	10	0.22334885603614651
1201	11	0.18628638680490184
1201	12	0.24012688491610135
1201	13	0.12729067403982697
1201	14	0.03400782817227821
1201	15	0.18328906746504003
1201	16	0.0407103683822001
1201	17	0.23961440591504868
1201	18	-0.028925564323422748
1201	19	-0.2865727343690878
1201	20	-0.25627451625399544
1201	21	0.025853097845676132
1201	22	0.036245555035492316
1201	23	0.05764262152162303
1201	24	0.04951228569859012
1201	25	0.003496272550790991
1202	1	-0.3216080282367173
1202	2	-0.31517989839379723
1202	3	-0.20841840130557898
1202	4	-0.11819278589503313
1202	5	-0.6236638578635194
1202	6	-0.45300490356486023
1202	7	-0.18452617009650396
1202	8	-0.22387620706313527
1202	9	-0.17001343181873096
1202	10	-0.1339251821637788
1202	11	-0.10766801271623905
1202	12	-0.01862046231141079
1202	13	-0.026937437186163038
1202	14	0.0201725993536499
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2210	1	0.1698612139223883
2210	2	0.18875176580505837
2210	3	0.2561237084444983
2210	4	0.23831549247352513
2210	5	0.2261420828193934
2210	6	0.06748349774532159
2210	7	0.10767892704532755
2210	8	0.01712190843019812
2210	9	0.05665458092411768
2210	10	0.13847576906339754
2210	11	0.04887503928717507
2210	12	0.04149866543122016
2210	13	0.18076211860869762
2210	14	-0.08408661005093876
2210	15	-0.006473903792894475
2210	16	0.08839607200295774
2210	17	0.04359910169705472
2210	18	0.10344658946529961
2210	19	0.08978038246554831
2210	20	0.20560491637011324
2210	21	0.03499830127979209
2210	22	0.25372409180293687
2210	23	0.29308495074894836
2210	24	0.2026944432200537
2210	25	0.5392372206657612
2211	1	0.24676119208356084
2211	2	0.21616859626294627
2211	3	0.03258509011444488
2211	4	0.18470116620141397
2211	5	0.22429488182936552
2211	6	0.22101956821194335
2211	7	0.07884257267392769
2211	8	0.019387686984465802
2211	9	-0.010044843986705132
2211	10	0.05632537232465751
2211	11	0.15070768105757537
2211	12	0.08673234090621662
2211	13	0.06392438111142695
2211	14	-0.03368441160877467
2211	15	0.20646378274710742
2211	16	-0.029078631352945195
2211	17	0.03171513867746967
2211	18	0.21153969567480857
2211	19	0.12015455440993605
2211	20	0.046199660491829775
2211	21	0.20775254165250345
2211	22	0.29646450091701126
2211	23	0.4241452259181955
2211	24	0.17944518938006127
2211	25	0.3022404236940801
2212	1	0.4148493258860739
2212	2	0.32409075496173045
2212	3	0.4573467352105922
2212	4	0.24312474352723967
2212	5	0.28058838852204815
2212	6	0.4188605286181897
2212	7	0.09386809092011106
2212	8	0.3067171748302826
2212	9	0.14268974069212703
2212	10	0.2834377633715448
2212	11	0.26610638767868267
2212	12	0.002632892168499268
2212	13	-0.015714699720490444
2212	14	0.12347219970445877
2212	15	0.09103951527839627
2212	16	0.31914105561187256
2212	17	0.11234257228316835
2212	18	0.08552897715938457
2212	19	0.11875296793332524
2212	20	0.02815796222911615
2212	21	0.06345303668091873
2212	22	0.0371411817557501
2212	23	0.08200476054036443
2212	24	0.07811772936148742
2212	25	-0.09811913222879554
2213	1	0.04518476871099608
2213	2	-0.055819703852691305
2213	3	0.019533256905816643
2213	4	0.05712481085143395
2213	5	0.35646276156543877
2213	6	0.2325323904689327
2213	7	-0.10644244195093222
2213	8	-0.14185134437393998
2213	9	-0.4033684274164031
2213	10	-0.3163703626593559
2213	11	-0.1574091137182947
2213	12	-0.24840642516412004
2213	13	-0.1761188132290954
2213	14	0.00492690734911605
2213	15	-0.19938445886911182
2213	16	-0.1395303013073672
2213	17	-0.19058266419308012
2213	18	-0.014363304121118858
2213	19	0.09060724470310788
2213	20	0.04303661035918793
2213	21	0.15011190496112192
2213	22	0.025258460511594194
2213	23	-0.028204954341653377
2213	24	0.004822714718471843
2213	25	-0.35384589696437985
2214	1	0.3848244942161472
2214	2	0.4394147605011014
2214	3	0.5408034310067222
2214	4	0.4693436442483119
2214	5	0.2801506507508478
2214	6	0.4248376389121944
2214	7	0.07682417388335949
2214	8	0.04675267138409822
2214	9	0.12560602931253584
2214	10	0.11776993471006847
2214	11	0.09247969044996296
2214	12	0.19312045245692389
2214	13	-0.02935620044475229
2214	14	0.2559276698348185
2214	15	-0.08159630281370056
2214	16	0.18906926661739476
2214	17	0.193741900554663
2214	18	0.24313447196224303
2214	19	0.2111594114333144
2214	20	0.2986281721840669
2214	21	0.20569393571471295
2214	22	0.11434220685801932
2214	23	0.10586544850496438
2214	24	0.21518934326085315
2214	25	0.008382467503786017
2215	1	0.21740906081233646
2215	2	0.19293836867587544
2215	3	0.06005114066140749
2215	4	-0.028630281051192696
2215	5	0.09326728218143643
2215	6	0.19527456632586393
2215	7	-0.038353768329471905
2215	8	0.08709798498522048
2215	9	-0.087484175056737
2215	10	-0.1407307700115652
2215	11	0.02235988777201925
2215	12	0.044688458094697125
2215	13	0.04041124141571828
2215	14	-0.13572137879096857
2215	15	-0.05442047476578438
2215	16	-0.38179930279768115
2215	17	0.031039293084617725
2215	18	0.20173207590868714
2215	19	0.1885642740125988
2215	20	0.3628018860670821
2215	21	0.3624504543898368
2215	22	0.2951913964281374
2215	23	0.23526995872342837
2215	24	0.2836354320557817
2215	25	0.24547725256368835
2216	1	0.2938263233251064
2216	2	0.30419134790638225
2216	3	0.21073636367808746
2216	4	0.3103635902460482
2216	5	0.2529753175917193
2216	6	0.4588939057879031
2216	7	0.21685988505834075
2216	8	0.3116468443655407
2216	9	0.34046100710192206
2216	10	-0.025923994390183225
2216	11	0.1279948894744365
2216	12	0.17896663310320804
2216	13	0.19584195302620344
2216	14	0.13666541044975133
2216	15	0.1675109178116756
2216	16	0.023099403365065996
2216	17	0.10317923179758992
2216	18	0.2388447759427308
2216	19	0.1945014548093269
2216	20	0.2571030437109414
2216	21	0.18069611322538748
2216	22	0.047361978654897996
2216	23	0.14236052733085103
2216	24	0.19217305548104235
2216	25	0.10347435845405073
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	26.0
9	72.0
10	128.0
11	153.0
12	197.0
13	243.0
14	413.0
15	571.0
16	816.0
17	933.0
18	1150.0
19	1196.0
20	1285.0
21	1337.0
22	1233.0
23	1153.0
24	1081.0
25	1075.0
26	1067.0
27	999.0
28	1122.0
29	1222.0
30	1480.0
31	1968.0
32	2534.0
33	3503.0
34	5259.0
35	8682.0
36	15617.0
37	38484.0
38	189548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.665120055677995	19.48800146225039	11.928940255120512	21.917938226951104
2	12.65560749305873	25.8981478227252	32.87878255368503	28.56746213053105
3	23.201006508567975	32.081757735074575	27.455121807217058	17.262113949140392
4	14.188115889059139	23.51026891772214	27.240746728144284	35.060868465074435
5	10.39100898254336	40.38437093949494	34.286198519857635	14.938421558104062
6	31.306280172853313	29.732808725646354	17.713948165103744	21.246962936396592
7	18.82299600378182	37.46568770451182	23.093361076061704	20.61795521564465
8	28.876323151102802	31.915529190154206	21.336646189746546	17.871501468996442
9	27.222312033906643	16.95051063799878	20.672580180357482	35.1545971477371
10	20.06178262917068	32.32377190331265	28.177869307598773	19.436576159917905
11	24.99648565444143	27.75243544147209	17.113808566568732	30.137270337517748
12	25.52065395401798	34.56594012918825	26.308927203334438	13.604478713459336
13	31.705722760307577	20.75361626157977	23.2024122467914	24.33824873132125
14	22.67385467478246	30.130241646400606	26.072929699031445	21.122973979785485
15	36.762163149978214	19.144397430310526	23.922150217186555	20.171289202524704
16	15.282834530553721	27.58901837299858	36.32884434260652	20.79930275384118
17	25.47875686636981	30.153687192265384	27.500975261739164	16.86658067962564
18	24.302573221904368	20.577692183337668	34.31887989991074	20.80085469484723
19	21.694131373727362	30.40306170861053	35.4043444492474	12.49846246841471
20	23.048132476770174	22.516060559202668	33.07139744436791	21.36440951965925
21	16.928078159869266	27.62691314203377	36.97727951642095	18.46772918167601
22	21.408012145791673	26.474030287162222	26.465947150342828	25.652010416703273
23	15.728453547380406	25.597087310401058	35.10409491544485	23.57036422677369
24	20.47858694200586	33.369297055660596	28.992500333865173	17.159615668468366
25	20.664252057106687	22.309234123356074	27.577888740417748	29.448625079119488
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3802.0
1	3802.0
2	3187.5
3	2573.0
4	2573.0
5	2573.0
6	3399.0
7	4225.0
8	4225.0
9	4225.0
10	3773.0
11	3321.0
12	3321.0
13	3321.0
14	2370.0
15	1419.0
16	1419.0
17	1419.0
18	2327.5
19	3236.0
20	3236.0
21	3236.0
22	4703.5
23	6171.0
24	6171.0
25	6171.0
26	8329.0
27	10487.0
28	10487.0
29	10487.0
30	11365.5
31	12244.0
32	12244.0
33	12244.0
34	12252.0
35	12260.0
36	12260.0
37	12260.0
38	10684.5
39	9109.0
40	9109.0
41	9109.0
42	12781.5
43	16454.0
44	16454.0
45	16454.0
46	55568.0
47	94682.0
48	94682.0
49	94682.0
50	79213.0
51	63744.0
52	63744.0
53	63744.0
54	43105.5
55	22467.0
56	22467.0
57	22467.0
58	14839.0
59	7211.0
60	7211.0
61	7211.0
62	6340.0
63	5469.0
64	5469.0
65	5469.0
66	4178.5
67	2888.0
68	2888.0
69	2888.0
70	2287.5
71	1687.0
72	1687.0
73	1687.0
74	1222.5
75	758.0
76	758.0
77	758.0
78	502.5
79	247.0
80	247.0
81	247.0
82	156.5
83	66.0
84	66.0
85	66.0
86	43.0
87	20.0
88	20.0
89	20.0
90	13.0
91	6.0
92	6.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019328900572135457
2	0.00632582200542615
3	0.0
4	0.0
5	0.4681108284015351
6	0.050958010599266204
7	0.010191602119853241
8	0.0
9	7.028691117140166E-4
10	7.028691117140166E-4
11	0.0
12	7.028691117140166E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.003162911002713075
18	7.028691117140166E-4
19	3.514345558570083E-4
20	0.0
21	0.0010543036675710249
22	0.0017571727792850414
23	0.0
24	7.028691117140166E-4
25	0.0590410053839774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	284548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.365801200500442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98212503402596	14.908205294010148
2	12.174938753289174	4.715548870489338
3	4.438798657109155	2.578826770878727
4	2.0470011795662826	1.5856727160268216
5	1.0561655022230287	1.0226745575438942
6	0.6478540967244352	0.7527728186457118
7	0.39742310135196446	0.5387491741287938
8	0.23228382179475549	0.3598689851975765
9	0.22321023500589784	0.3890380533337082
>10	1.346520279466473	5.244809311609992
>50	0.19780419199709648	2.650519420273557
>100	0.15606569276835133	6.522625356706074
>500	0.02359132565102985	3.005116887133278
>1k	0.0598856728064604	28.54456893037379
>5k	0.01270302150440069	15.783980207205813
>10k+	0.0036294347155430545	11.39702264644278
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	16430	5.774069752730647	No Hit
CCCATGTACTCTGCGTTGATACCAC	16000	5.6229528937121325	No Hit
GAGTACATGGGAAGCAGTGGTATCA	8532	2.998439630571995	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7160	2.5162714199361793	No Hit
CATGTACTCTGCGTTGATACCACTG	6804	2.3911607180510845	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5966	2.0966585602429118	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5796	2.0369146857472202	No Hit
GCGTTGATACCACTGCTTCCCATGT	5629	1.9782251149190997	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5026	1.7663100777373235	No Hit
GGTATCAACGCAGAGTACATGGGAA	4982	1.7508469572796153	No Hit
TATCAACGCAGAGTACATGGGAAGC	4942	1.736789575045335	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4803	1.687940171781211	No Hit
GTATCAACGCAGAGTACATGGGAAG	4426	1.5554493442231188	No Hit
GTGGTATCAACGCAGAGTACATGGG	3531	1.2409154167310965	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3304	1.1611397725515555	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3165	1.1122903692874313	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3006	1.0564122749061668	No Hit
GTACTCTGCGTTGATACCACTGCTT	2958	1.0395434162250305	No Hit
GATACCACTGCTTCCCATGTACTCT	2821	0.9913968820726204	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2676	0.9404388714733543	No Hit
CAGTGGTATCAACGCAGAGTACATG	2563	0.9007267666615124	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2513	0.8831550388686618	No Hit
ATACCACTGCTTCCCATGTACTCTG	2446	0.8596089236262424	No Hit
CATGGGAAGCAGTGGTATCAACGCA	2431	0.8543374052883872	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	2315	0.8135709968089742	No Hit
ACATGGGAAGCAGTGGTATCAACGC	2211	0.7770218029998454	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	2187	0.7685873736592771	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2088	0.7337953526294333	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2016	0.7084920646077287	No Hit
GTTGATACCACTGCTTCCCATGTAC	2012	0.7070863263843008	No Hit
TACCACTGCTTCCCATGTACTCTGC	1935	0.6800258655833111	No Hit
CTTCCCATGTACTCTGCGTTGATAC	1816	0.6382051534363271	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1811	0.6364479806570421	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1673	0.5879500119487748	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	1619	0.5689725459324965	No Hit
CCATGTACTCTGCGTTGATACCACT	1586	0.5573752055892152	No Hit
TGATACCACTGCTTCCCATGTACTC	1404	0.4934141164232396	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1319	0.463542179175394	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1261	0.44315897493568746	No Hit
TTCCCATGTACTCTGCGTTGATACC	1256	0.44140180215640246	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1088	0.382360796772425	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1059	0.3721691946525718	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	913	0.3208597494974486	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	855	0.30047654525774214	No Hit
ACCATGTACTCTGCGTTGATACCAC	848	0.298016503366743	No Hit
CTGCGTTGATACCACTGCTTCCCAT	677	0.23792119431519462	No Hit
TCAACGCAGAGTACATGGGAAGCAG	651	0.2287838958629124	No Hit
CTCTGCGTTGATACCACTGCTTCCC	649	0.22808102675119837	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	635	0.22316094296920028	No Hit
AAAAAGTACTCTGCGTTGATACCAC	627	0.22034946652234422	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	587	0.20629208428806386	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	532	0.18696318371592843	No Hit
AGTGGTATCAACGCAGAGTACATGG	527	0.18520601093664338	No Hit
TCCATGTACTCTGCGTTGATACCAC	526	0.18485457638078637	No Hit
CCCCATGTACTCTGCGTTGATACCA	524	0.18415170726907235	No Hit
AAAGTACTCTGCGTTGATACCACTG	476	0.16728284858793596	No Hit
AACGCAGAGTACATGGGAAGCAGTG	473	0.16622854492036493	No Hit
TCCCATGTACTCTGCGTTGATACCA	446	0.1567398119122257	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	425	0.14935968623922852	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	419	0.14725107890408648	No Hit
ATGTACTCTGCGTTGATACCACTGC	417	0.14654820979237246	No Hit
GTACATGGGTAAGCAGTGGTATCAA	406	0.1426824296779454	No Hit
GAGTACATGGTAAGCAGTGGTATCA	404	0.14197956056623137	No Hit
GCTTACCATGTACTCTGCGTTGATA	399	0.14022238778694632	No Hit
GTACATGGAAAGCAGTGGTATCAAC	392	0.13776234589594727	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	391	0.13741091134009026	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	390	0.13705947678423325	No Hit
TTGATACCACTGCTTCCCATGTACT	375	0.1317879584463781	No Hit
CAACGCAGAGTACATGGGAAGCAGT	371	0.13038222022295007	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	352	0.12370496366166692	No Hit
ACTCTGCGTTGATACCACTGCTTAC	334	0.11737914165624078	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	333	0.11702770710038378	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	329	0.11562196887695572	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	327	0.11491909976524171	No Hit
CGTTGATACCACTGCTTCCCATGTA	302	0.1061332358688165	No Hit
GAGTACATGGAAAGCAGTGGTATCA	291	0.10226745575438942	No Hit
GGTATCAACGCAGAGTACATGGGGG	285	0.10015884841924738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	7.028691117140166E-4	0.0	0.0	0.0	0.0
9	7.028691117140166E-4	0.0	0.0	0.0	0.0
10	7.028691117140166E-4	0.0	0.0	3.514345558570083E-4	0.0
11	7.028691117140166E-4	0.0	0.0	3.514345558570083E-4	0.0
12	7.028691117140166E-4	0.0	0.0	3.514345558570083E-4	0.0
13	7.028691117140166E-4	0.0	0.0	7.028691117140166E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGATTC	35	0.0021820785	16.265831	9
GAACAAA	105	8.185452E-11	14.52745	1
GGCGCGC	40	0.0053079342	14.232601	8
GGTCAGT	60	2.5833328E-5	14.232601	17
TCAGTCA	65	5.4845197E-5	13.137787	19
GTCAGTC	65	5.4845197E-5	13.137787	18
AAAAGTA	125	1.3151293E-9	12.200904	2
CGGTCAG	70	1.0973155E-4	12.199373	16
AAAAAGT	130	2.4319888E-9	11.73371	1
TCGGTCA	75	2.0860211E-4	11.386081	15
GAAAAAA	300	0.0	10.804792	1
TATCGGT	85	6.6318194E-4	10.046541	13
ATCGGTC	85	6.6318194E-4	10.046541	14
CTATCGG	90	0.0011200841	9.4884	12
GTACTGG	115	1.0601418E-4	9.119134	1
ACTGGTT	95	0.0017592951	9.030274	3
GTTCACT	95	0.001834226	8.989011	7
AACAAAA	310	0.0	8.6095085	2
TACTGGT	100	0.002802829	8.57876	2
TTCACTA	100	0.0029215766	8.539561	8
>>END_MODULE