FastQCFastQC Report
Wed 25 May 2016
SRR1294834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758305
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.012.67001151
CGAGCAC550.003064692312.0936173
ACCGTCT1552.5465852E-1111.6424898
TCGTCCG1001.9371237E-611.3973859
CGTCCGC1104.9903974E-711.224545510
CCGTGCA1606.206392E-910.09143459
GTATTAG5200.010.050731
GTATCAA28000.010.0115711
TACGACA900.00111119189.5028894
GGGTACG800.00450813269.5025091
CTACGAC1002.7509747E-49.5021293
TCGTCGC1106.8598165E-59.49769210
GTCGTAT1151.1082115E-49.0893551
ATCGTCG1151.114184E-49.0848719
GTCCGCA1151.1154792E-49.08390111
TGGACTG7150.09.037235
CTAGTAC2002.6484486E-99.0270213
GCGCGTC950.00182852328.9979359
CGTCTGG1804.240792E-88.97004310
TTGCGTG1804.267531E-88.96657716
Produced by FastQC (version 0.11.5)