FastQCFastQC Report
Wed 25 May 2016
SRR1294834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758305
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110450.29388248159742225No Hit
GGTATCAACGCAGAGTACTTTTTTT63850.1698904160253093No Hit
TATCAACGCAGAGTACTTTTTTTTT62340.16587264737694252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.013.9000471
TGCGCTA500.001514338213.28349710
GCGGGTA500.001514338213.2834979
TAACGCT603.98497E-412.7074164
TCGTCCG1103.8864528E-812.0759079
GTCCGCA1201.2989585E-711.06972811
CCGTTTA700.001507789110.8436729
GTATCAA41150.010.4480561
AACCGTG750.00267530610.1212997
GCGCGTC750.002676487210.120769
GTTCTAG4100.09.9980061
CTAGACA3450.09.9449354
CTACCCG1054.1648425E-59.94003210
TACCCGC1252.6133184E-69.86787211
TCTATAC4400.09.5305613
TTTCGTC800.00456396779.48833817
GGTTCGT800.00456396779.48833814
GTATTAT5900.09.3714071
TCGTCTA1451.7367292E-69.16115514
TCCGTTT1054.551347E-49.0363928
Produced by FastQC (version 0.11.5)