FastQCFastQC Report
Wed 25 May 2016
SRR1294833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661589
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70550.2650672211224197No Hit
CCCATGTACTCTGCGTTGATACCAC64140.24098386339889444No Hit
GAGTACATGGGAAGCAGTGGTATCA33900.12736752368603868No Hit
ATTCCATTCCATTCCATTCCATTCC29840.11211347807644231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGG400.00529594614.2436918
GTCCTAA2400.012.2720991
GCCGCTC550.003074423412.0882858
TAGGACT3350.011.3450944
TACGTCT600.005870539711.0851024
ACCGTTC600.00588701911.0809298
GTATTAG3650.010.9326331
CCGTTCA700.001494708910.8547879
GCACCGT1158.8718843E-710.7345766
CGCGATC1002.7719195E-49.49472117
ATAGGAC2451.8189894E-129.3067323
CCTATAC3100.09.194153
TATACTG5750.09.0880645
TGCTCCG1054.4886454E-49.0487225
GTCCTAG2651.8189894E-128.9631881
TGGACCA2451.6370905E-118.9194565
GTACTGG3550.08.8318971
GTAGGAC2304.0381565E-108.674483
ACCGTGT1651.0537551E-68.634498
GTATAGA4200.08.5961241
Produced by FastQC (version 0.11.5)