FastQCFastQC Report
Wed 25 May 2016
SRR1294833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661589
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89200.33513814492019617No Hit
GTACATGGGAAGCAGTGGTATCAAC71920.27021452222713577No Hit
CCCATGTACTCTGCGTTGATACCAC65930.24770916922184455No Hit
GGTATCAACGCAGAGTACTTTTTTT57890.21750165032993451No Hit
TATCAACGCAGAGTACTTTTTTTTT52420.19695001745198074No Hit
GAGTACATGGGAAGCAGTGGTATCA35680.1340552579680785No Hit
ATTCCATTCCATTCCATTCCATTCC28190.10591417382623687No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28000.1052003145489405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGCG400.005164507314.2977485
GCGCTGA1053.506717E-610.8472526
GTCTCGC1605.815309E-910.1310131
CATCGCT750.002677896810.1199189
CGGTCGT750.002677896810.11991810
GTCTTAC2800.09.875611
TTTAGAC2550.09.718733
TCTCGCT1606.2969775E-89.5327312
TGCGCTG1201.6352287E-59.5318315
GTTCTAG2750.09.3617061
TAATACT5250.09.2594944
GTCTAGA2700.09.181921
GCCTTAG2304.0017767E-119.1205021
TTACACT5250.09.0779354
GTCTTAG2850.09.0332261
AGCGGTG850.00749000638.93353419
CCGGTCG850.00751891978.9293389
AGCTCGG850.00751891978.92933810
TCTGTCG1606.87447E-78.8944598
CTCCTAT3450.08.8441241
Produced by FastQC (version 0.11.5)