FastQCFastQC Report
Wed 25 May 2016
SRR1294832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630505
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31310.11902657474515349No Hit
GTATCAACGCAGAGTACTTTTTTTT26550.10093119001864662No Hit
CCCATGTACTCTGCGTTGATACCAC26440.10051301936320212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC907.4551845E-611.6126133
CGGTAGG600.00590355611.07674516
CTAGAAC2750.011.0559763
TAGACCG700.00149038510.8585485
GACGCTT803.6257785E-410.73348319
GTATTAG3350.010.4942951
GTCGTAT1002.3956658E-510.4517521
TCGTATC951.6420247E-410.0016762
TGGACGG2107.2759576E-129.953675
TTAGACT2251.8189894E-129.7125534
CAGGACT4050.09.6187114
AGCACCG900.0011127349.5012295
ACGACGA1002.7528795E-49.5012295
TGTGCGA800.0045244569.49815110
GCGTCAA1304.2534575E-69.49778911
GTTCTAA2054.2200554E-109.2698461
CTTAGAC1752.6659109E-89.2297663
CTAATAC2401.0913936E-119.1053453
GTCTAGA2201.6734703E-109.0697011
ACACCGT1054.5191398E-49.0425856
Produced by FastQC (version 0.11.5)