FastQCFastQC Report
Wed 25 May 2016
SRR1294832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630505
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92360.3511112885168437No Hit
GGTATCAACGCAGAGTACTTTTTTT62040.2358482496706906No Hit
TATCAACGCAGAGTACTTTTTTTTT53890.20486560565366727No Hit
GTACATGGGAAGCAGTGGTATCAAC30630.11644151978422393No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27230.10351624497957616No Hit
CCCATGTACTCTGCGTTGATACCAC27100.10302204329586905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG456.833625E-414.7579819
TCTCGTA855.3688527E-511.16765419
TAACACG600.005729618511.1213354
CGCCTAA600.005937261511.06827614
GTCCTAA1951.8189894E-1210.7598361
TGGACGG3000.010.1680785
ATCGACC750.002678227110.119768
CTGGACG3500.010.07729154
GTGCTAG2400.09.9345071
ACGGGGA2700.09.83865458
ATTAGAC1951.5279511E-109.77699853
GGACGGG3500.09.7637216
CTAGCAC2550.09.7194873
CTAAGAC3050.09.6888453
GTCTCGG800.00437414979.53712751
GTCTTAG3000.09.5371271
TGAAGCG800.00439142169.5325745
TAGGACA3300.09.5325734
AGACCGT900.00112212129.4925076
GACGCTT800.00454669869.49250619
Produced by FastQC (version 0.11.5)