FastQCFastQC Report
Wed 25 May 2016
SRR1294831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664747
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC43090.16170390659976352No Hit
CCCATGTACTCTGCGTTGATACCAC40650.15254731499838448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGT250.006026835619.0034624
AGAACCG700.001490208610.8587125
GTCTAAG2200.010.796611
CGAACAT803.7938185E-410.68100715
TATTCCG1002.3963068E-510.4515115
CGTGTAC750.002646694210.1342273
TCCGTAC951.6492956E-49.9973088
GTCCTAA2002.6375346E-109.5010171
ACGTCGC800.00453641369.4951216
GTCCTAC2151.05501385E-109.2800631
CTGCTCG1651.0707481E-79.2096419
GTCTAGC1554.256326E-79.1945311
GCACTGT6250.09.1153166
TACACCG1151.1093292E-49.088275
ACACCGT1054.5172565E-49.0429736
TTCCGTA950.00182882088.9975777
AGCACCG1701.6821832E-78.9424695
CACCGTT850.0074541038.9387697
GTATAAA5250.08.8676151
GTCTTAC3150.08.7469671
Produced by FastQC (version 0.11.5)