FastQCFastQC Report
Wed 25 May 2016
SRR1294831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664747
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86540.324758785730878No Hit
GGTATCAACGCAGAGTACTTTTTTT55940.20992612056604248No Hit
TATCAACGCAGAGTACTTTTTTTTT49420.18545850694268537No Hit
GTACATGGGAAGCAGTGGTATCAAC45590.17108566028970104No Hit
CCCATGTACTCTGCGTTGATACCAC41170.15449871976589147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCG508.7820656E-515.18688719
CGCAACC400.005329939514.229959518
GAACCGT701.10029185E-412.203756
GTCCGGA1052.5881855E-711.8078431
TACATCG1052.5976988E-711.8045035
AACTGCG658.1156474E-411.6769617
AGAACCG752.0063523E-411.4412895
TGCGCTG1303.658206E-710.2678225
GTCGTAT750.002564337810.1729121
ACGGGTC750.00257036510.1700345
GAACCGC951.6592068E-49.9913746
CTGGACG1951.5279511E-109.7788794
CTACACT4150.09.6492794
GTCTAAG2600.09.5371051
AACCGTG900.00112752539.487537
GTCGTAG1151.0648831E-49.1224481
TAGTCCG1151.06817075E-49.1198685
CCTACAC3150.09.0803883
GTCGTCC1151.1282391E-49.0741788
GTCTTAG1909.902578E-99.0351521
Produced by FastQC (version 0.11.5)